Physical exercise improves learning in zebrafish, Danio rerio

Luchiari, Ana Carolina; Chacon, Diana Marques Martins

doi:10.1016/j.beproc.2013.07.020

Cited by 29 publications

(19 citation statements)

References 30 publications

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“…Different letters indicate statistical significance for the interaction term treatment by area of the tank (Tukey post-hoc tests, p < .05) conditioning. Our results showed the associative-learning ability of zebrafish, corroborating other literature studies (Al-Imari & Gerlai, 2008;Braubach, Wood, Gadbois, Fine, & Croll, 2009;Chacon & Luchiari, 2014;Gómez-Laplaza & Gerlai, 2010;Karnik & Gerlai, 2012;Luchiari & Chacon, 2013). In addition, we showed that fish can discriminate a visual target in the presence of distractors and that their performance in terms of time to reach the correct choice improved at a low dose of caffeine (10 mg/L).…”

Section: Discussionsupporting

confidence: 91%

Coffee time: Low caffeine dose promotes attention and focus in zebrafish

2019

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In this study we investigated the ability of zebrafish to discriminate visual signs and associate them with a reward in an associative-learning protocol including distractors. Moreover, we studied the effects of caffeine on animal performance in the task. After being trained to associate a specific image pattern with a reward (food) in the presence of other, distractor images, the fish were challenged to locate the exact cue associated with the reward. The distractors were same-colored pattern images similar to the target. Both the target and distractors were continually moved around the tank. Fish were exposed to three caffeine concentrations for 14 days: 0 mg/L (control, n = 12), 10 mg/L (n = 14), and 50 mg/L (n = 14). Zebrafish spent most of the time close to the target (where the reward was offered) under the effects of 0 and 10 mg/L caffeine, and the shortest latency to reach the target was observed for the 10-mg/L caffeine group. Both caffeine treatments (10 and 50 mg/L) increased the average speed and distance traveled when compared to the control group. This study confirms previous results showing that zebrafish demonstrate conditioned learning ability; however, low-dose caffeine exposure seems to favor visual cue discrimination and to increase zebrafish performance in a multicue discrimination task, in which primarily focus and attention are required in order to obtain the reward.Caffeine is one of the most consumed stimulants in the world (Ferré, 2008;Lieberman, 1992). It is present in a wide range of products, including coffee, energy drinks, teas, and chocolate. The popularity of this substance lies in its beneficial effects, such as heightened attention and alertness and decreased fatigue

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Section: Discussionsupporting

confidence: 91%

Coffee time: Low caffeine dose promotes attention and focus in zebrafish

2019

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“…which mirrors phenomena in humans [24][25][26][27] . However, the effect of diet on cognitive function in zebrafish is not well known.…”

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confidence: 58%

High-fat diet impairs cognitive function of zebrafish

Meguro

Hosoi

Hasumura

2019

Sci Rep

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An unhealthy diet with excessive fat intake has often been claimed to induce not only obesity but also cognitive dysfunction in mammals; however, it is not known whether this is the case in zebrafish. Here, we investigated the effect of excessive fat in the diet on cognitive function and on gene expression in the telencephalon of zebrafish. Cognitive function, as measured by active avoidance test, was impaired by feeding of a high-fat diet compared with a control diet. In RNA sequencing analysis of the telencephalon, 97 genes were identified with a fold change in expression greater than 2 and a p-value less than 0.05 between the two diets. In quantitative real-time PCR analysis of the telencephalon, genes related to neuronal activity, anti-oxidative stress, blood-brain barrier function and amyloid-β degradation were found to be downregulated, whereas genes related to apoptosis and amyloid-β production were found to be upregulated, in the high-fat diet group, which are changes known to occur in mammals fed a high-fat diet. Collectively, these results are similar to those found in mammals, suggesting that zebrafish can serve as a suitable animal model in research into cognitive impairment induced by excessive fat in the diet.According to the World Health Organization, in December 2017 around 50 million people had dementia, and 10 million new cases emerge every year 1 . Dementia is a syndrome-usually of a chronic or progressive nature-in which there is deterioration in cognitive function 1 . Some research has shown a relationship between the development of cognitive impairment and life-style-related risk factors that are shared with other non-communicable diseases 1 . Obesity and unhealthy diet are two risk factors for dementia 1,2 . Excessive fat intake has often been claimed to induce obesity in humans and rodents 3,4 . Although numerous studies have been conducted to elucidate the effects of obesity and excessive dietary fat intake on cognitive function in rodents [5][6][7] , no such studies have been conducted in zebrafish (Danio rerio).The organs and tissues of zebrafish are similar in terms of structure and function to those of humans. In addition, these fish are amenable to genetic manipulation, breed readily in captivity, and can be inexpensively maintained, making them a useful model organism for investigating many human pathological conditions 8 . Several reports suggest that zebrafish are a suitable model organism for examining the mechanisms underlying lipid metabolism leading to obesity. For example, overexpression of the endogenous melanocortin antagonist agouti-related protein or the serine/threonine protein kinase Akt1 results in the development of adiposity in zebrafish 9,10 . Overfeeding and a high-fat (HF) diet induce body fat accumulation in zebrafish through pathophysiological pathways common with those underlying mammalian obesity; furthermore, green tea extract and fish oil, which have well-known anti-obesity activities, can cancel out these effects. Thus, zebrafish are a useful model of human...

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“…The training tube consisted in transparent tunnel connected to a 180 L/h submerse water pump (Moto Bomba SARLO S300, 220 V, 60 Hz. Sarlobetter Equipaments Ltda., São Caetano do Sul-SP) placed inside a glass tank [32]. Each group was trained for 30 min, during which the first 15 min fish could not exit the tube and was forced to remain swimming against the water current.…”

Section: Groups and Trainingmentioning

confidence: 99%

“…Regarding physical activity, zebrafish swimming ability improves with training [31] and enhances associative learning response [32]; therefore, it offers a good organism model to the study of physical activity on cognition.…”

Section: Introductionmentioning

confidence: 99%

Zebra Fitness: Learning and Anxiety After Physical Exercise in Zebrafish

Silveira¹,

Silveira²,

Agues-Barbosa³

et al. 2018

Recent Advances in Zebrafish Researches

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In the recent years, a new branch of physical training has emerged, the high-intensity interval training (HIIT). In contrast to continued exercise regime used in most of the trainings, HIIT proposes a regime of short periods of maximum intensity exercising and brief less intense recovery periods, which are repeated until complete exhaustion. HIIT is calling the attention of those who search for fast escalation in physical performance; however, the stress caused by this type of training may affect other systems functioning, such as cognition. Thus, we investigated the effects of two physical regime protocols, traditional endurance and HIIT on zebrafish learning, memory, and anxiety-like behavior. To that, fish were trained for 30 days and submitted to a latent learning test, objects discrimination test, and novel tank test. Our results showed that HIIT does not affect long lasting memory, evaluated through the latent learning task, but it impairs discriminative learning. On the other hand, both training protocols decrease anxiety-like behavior. This study confirms that zebrafish show good performance in learning tasks and that cognitive performance is dependent upon the regime of physical exercise and cognitive task used.

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Physical exercise improves learning in zebrafish, Danio rerio

Cited by 29 publications

References 30 publications

Coffee time: Low caffeine dose promotes attention and focus in zebrafish

Coffee time: Low caffeine dose promotes attention and focus in zebrafish

High-fat diet impairs cognitive function of zebrafish

Zebra Fitness: Learning and Anxiety After Physical Exercise in Zebrafish

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