Physical forces during collective cell migration

Trepat, Xavier; Wasserman, Mark; Angelini, Thomas E.; Millet, Emil; Weitz, David A.; Butler, James P.; Fredberg, Jeffrey J.

doi:10.1038/nphys1269

Cited by 1,089 publications

(1,433 citation statements)

References 39 publications

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“…3.1). However, values are consistent with the experimental observations (Serra-Picamal et al 2012;Tambe et al 2011;Trepat et al 2009) for the second, the third and the fourth modes of migration, which are also the most efficient modes of collective movement (Sect. 3.1).…”

Section: Principal Stressessupporting

confidence: 91%

“…Although the stress regime may vary according to the mode of migration adopted by the cohort, few general remarks may be pointed out. leading (c(18, 4) (Serra-Picamal et al 2012;Tambe et al 2011;Trepat et al 2009), which confirms the inefficiency of these migration modes (Sect. 3.1).…”

Section: Principal Stressessupporting

confidence: 67%

“…In fact, the distinction between leader (located at the free boundary) and follower (located in the cell cohort) cells has been thought to be the main feature of collective cell migration (Friedl and Gilmour 2009) with the former exerting larger traction forces than the latter (Trepat et al 2009). Nevertheless, further studies have shown that both cells actually generate protrusions to coordinate the forward movement (Farooqui and Fenteany 2005;Fenteany et al 2000;Tambe et al 2011) and that the cell velocity is inversely proportional to the distance from the free boundary (Farooqui and Fenteany 2005).…”

Section: Introductionmentioning

confidence: 99%

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On the Mechanical Interplay Between Intra- and Inter-Synchronization During Collective Cell Migration: A Numerical Investigation

2013

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is an open access repository that collects the work of Arts et Métiers ParisTech researchers and makes it freely available over the web where possible. Abstract Collective cell migration is a fundamental process that takes place during several biological phenomena such as embryogenesis, immunity response, and tumorogenesis, but the mechanisms that regulate it are still unclear. Similarly to collective animal behavior, cells receive feedbacks in space and time, which control the direction of the migration and the synergy between the cells of the population, respectively. While in single cell migration intra-synchronization (i.e. the synchronization between the protrusion-contraction movement of the cell and the adhesion forces exerted by the cell to move forward) is a sufficient condition for an efficient migration, in collective cell migration the cells must communicate and coordinate their movement between each other in order to be as efficient as possible (i.e. intersynchronization). Here, we propose a 2D mechanical model of a cell population, which is described as a continuum with embedded discrete cells with or without motility phenotype. The decomposition of the deformation gradient is employed to reproduce the cyclic active strains of each single cell (i.e. protrusion and contraction). We explore different modes of collective migration to investigate the mechanical interplay between intra-and inter-synchronization. The main objective of the paper is to evaluate the efficiency of the cell population in terms of covered distance and how the

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Section: Principal Stressessupporting

confidence: 91%

Section: Principal Stressessupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

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On the Mechanical Interplay Between Intra- and Inter-Synchronization During Collective Cell Migration: A Numerical Investigation

2013

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“…Examples range from the cytoskeleton that controls cell motion [2], to bacterial suspensions [3] and mammalian tissues [4,5], to animal groups [6]. For the physicist, active materials are an exciting new class of nonequilibrium systems in which the interplay of activity, noise and interactions gives rise to a wealth of novel phases with unusual structural, dynamical and mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Freezing and phase separation of self-propelled disks

2014

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We study numerically a model of non-aligning self-propelled particles interacting through steric repulsion, which was recently shown to exhibit active phase separation in two dimensions in the absence of any attractive interaction or breaking of the orientational symmetry. We construct a phase diagram in terms of activity and packing fraction and identify three distinct regimes: a homogeneous liquid with anomalous cluster size distribution, a phase-separated state both at high and at low density, and a frozen phase. We provide a physical interpretation of the various regimes and develop scaling arguments for the boundaries separating them.

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“…Interestingly, a similar behavior of cells expanding in the perpendicular direction was also observed by another report in 2009. 37 …”

Section: Effects Of Geometry and Size Of Polymer Structures On Cellsmentioning

confidence: 99%

Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics

et al. 2014

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The culturing of cancer cells on micropatterned substrates can provide insight into the factors of the extracellular environment that enable the control of cell growth. We report here a novel non-UV-based technique to quickly micropattern a poly-(ethylene) glycol diacrylate (PEGDA)-based hydrogel on top of modified glass substrates, which were then used to control the growth patterns of breast cancer cells. Previously, the fabrication of micropatterned substrates required relatively complicated steps, which made it impractical for researchers to rapidly and systematically investigate the effects of different cell growth patterns. The technique presented herein operates on the principle of optically-induced electrokinetics (OEKs) and uses computer-generated projection light patterns to dynamically pattern the hydrogel on a hydrogenated amorphous silicon (a-Si:H) thin-film, atop an indium tin oxide (ITO) glass substrate. This technique allows us to pattern lines, circles, pentagons, and more complex shapes in the hydrogel with line widths below 3 μm and thicknesses of up to 6 μm within 8 s by simply controlling the projected illumination pattern and applying an appropriate AC voltage between the two ITO glass substrates. After separating the glass substrates to expose the patterned hydrogel, we experimentally demonstrate that MCF-7 breast cancer cells will adhere to the bare a-Si:H surface, but not to the hydrogel patterned in various geometric shapes and sizes. Theoretical analysis and finite-element model simulations reveal that the dominant OEK forces in our technique are the dielectrophoresis (DEP) force and the electro-osmosis force, which enhance the photo-initiated cross-linking reaction in the hydrogel. Our preliminary cultures of breast cancer cells demonstrate that this reported technique could be applied to effectively confine the growth of cancer cells on a-Si:H surfaces and affect individual cell geometry during their growth.

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Physical forces during collective cell migration

Cited by 1,089 publications

References 39 publications

On the Mechanical Interplay Between Intra- and Inter-Synchronization During Collective Cell Migration: A Numerical Investigation

On the Mechanical Interplay Between Intra- and Inter-Synchronization During Collective Cell Migration: A Numerical Investigation

Freezing and phase separation of self-propelled disks

Extracellular-controlled breast cancer cell formation and growth using non-UV patterned hydrogels via optically-induced electrokinetics

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