Physical Localization of 5S rRNA Genes in the Pig by Fluorescence in Situ Hybridization

Mellink, C.; Bosma, Anneke A.; Haan, N.A. de; Zijlstra, C.

doi:10.1111/j.1601-5223.1996.00095.x

Cited by 20 publications

(13 citation statements)

References 13 publications

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“…In man and in American mink 5S rRNA genes are organised in more than one cluster (Kost et al, 1995;Lomholt et al, 1995a;Christensen et al, 1998) while in the mouse, the rat and in the pig only one cluster was detected (Matsuda et al, 1994;Lomholt et al, 1995b;Mellink et al, 1996;Suzuki et al, 1996). The present study revealed the existence of a single 5S rRNA gene cluster for the maned wolf (proximal on 5q3), for the bat-eared fox (proximal on chromosome 19q5) and the fennec fox (distal on chromosome 12q6).…”

Section: Discussionmentioning

confidence: 62%

Cytogenetic studies and karyotype nomenclature of three wild canid species: maned wolf (Chrysocyon brachyurus), bat-eared fox (Otocyon megalotis) and fennec fox (Fennecus zerda)

Pieńkowska-Schelling¹,

Schelling

Zawada

et al. 2008

Cytogenet Genome Res

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We have analysed the chromosomes of three wild and endangered canid species: the maned wolf (Chrysocyon brachyurus), the bat-eared fox (Otocyon megalotis) and the fennec fox (Fennecuszerda) using classical and molecular cytogenetic methods. For the first time detailed and encompassing descriptions of the chromosomes are presented including the chromosomal assignment of nucleolar organizer regions and the 5S rRNA gene cluster. We propose a karyotype nomenclature with ideograms including more than 300 bands per haploid set for each of these three species which will form the basis for further research. In addition, we propose four basic different patterns of karyotype organization in the family Canidae. A comparison of these patterns with the most recent molecular phylogeny of Canidae revealed that the karyotype evolution of a species is not always strongly connected with its phylogenetic position. Our findings underline the need and justification for basic cytogenetic work in rare and exotic species.

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Section: Discussionmentioning

confidence: 62%

Cytogenetic studies and karyotype nomenclature of three wild canid species: maned wolf (Chrysocyon brachyurus), bat-eared fox (Otocyon megalotis) and fennec fox (Fennecus zerda)

Pieńkowska-Schelling¹,

Schelling

Zawada

et al. 2008

Cytogenet Genome Res

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“…In almost all the fish species studied, the location of the 5S rDNA occurs at an interstitial position in the chromosomes. This same 5S rDNA chromosomal location has been observed in mammals (Mellink et al, 1996;Frederiksen et al, 1997;Mäkinem et al, 1997; among others) and amphibians (Vitelli et al, 1982;Schmid et al, 1987;Lucchini et al, 1993), suggesting that such organization may not be casual. An interstitial distribution for the 5S rRNA gene loci could represent some advantage related to the organization of these genes in the vertebrate genome (Martins and Galetti, 2001b).…”

Section: Chromosome Locationmentioning

confidence: 52%

Dynamics of 5S rDNA in the tilapia (Oreochromis niloticus) genome: repeat units, inverted sequences, pseudogenes and chromosome loci

Martins

Wasko

Oliveira

et al. 2002

Cytogenet Genome Res

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In higher eukaryotes, the 5S ribosomal DNA (5S rDNA) is organized in tandem arrays with repeat units composed of a coding region and a non-transcribed spacer sequence (NTS). These tandem arrays can be found on either one or more chromosome pairs. 5S rDNA copies from the tilapia fish, Oreochromis niloticus, were cloned and the nucleotide sequences of the coding region and of the non-transcribed spacer were determined. Moreover, the genomic organization of the 5S rDNA tandem repeats was investigated by fluorescence in situ hybridization (FISH) and Southern blot hybridization. Two 5S rDNA classes, one consisting of 1.4-kb repeats and another one with 0.5-kb repeats were identified and designated 5S rDNA type I and type II, respectively. An inverted 5S rRNA gene and a 5S rRNA putative pseudogene were also identified inside the tandem repeats of 5S rDNA type I. FISH permitted the visualization of the 5S rRNA genes at three chromosome loci, one of them consisting of arrays of the 5S rDNA type I, and the two others corresponding to arrays of the 5S rDNA type II. The two classes of the 5S rDNA, the presence of pseudogenes, and the inverted genes observed in the O. niloticus genome might be a consequence of the intense dynamics of the evolution of these tandem repeat elements.

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“…Although the chromosome structure of the 5S rDNA clusters of these families seems to have been preserved from major changes during evolution, their nucleotide sequence seems to be evolving fast. Martins and Wasko (2004) have pointed out that in piscines the 5S rDNA repeat clusters are most commonly located at interstitial chromosome sites, and this has also been reported for mammals (Mellink et al, 1996;Frederiksen et al, 1997;Mäkinem et al, 1997) and amphibians (Vitelli et al, 1982;Schmid et al, 1987;Lucchini et al, 1993), suggesting that such distribution could represent some advantage related to the organization of these genes in the vertebrate genome (Martins and Galetti, 1999). It is interesting to note that even in other piscine species with high rates of chromosome variations such as the genus Astyanax (Characidae), the 5S rDNA chromosome clusters are conserved among species (Almeida-Toledo et al, 2002;Mantovani et al, 2005).…”

Section: Resultsmentioning

confidence: 86%

Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)

et al. 2006

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The 5S ribosomal DNA (5S rDNA) of higher eukaryotes is organized in repeat units of tandem arrays composed of a 5S rDNA coding region, conserved even among non-related taxa, and a variable non-transcribed spacer sequence (NTS). To contribute to knowledge on the organization and evolution of vertebrate 5S rDNA we used PCR, nucleotide sequencing, Southern blot hybridization and chromosome fluorescence in situ hybridization (FISH) to investigate 5S rDNA tandem repeats in the South American Curimatidae fish Steindachnerina insculpta and Cyphocharax modesta. 5S rDNA repeats of 180 base pairs (bp) from both species were PCR-generated and sequenced evidencing the shortest 5S rDNA monomer so far described in eukaryote species. Southern blotting revealed that both species contained two tandem 5S rDNA classes, the PCR amplified fragment composed of 180 bp monomers and a class of 1600 bp monomers not detected by PCR. Chromosome mapping of the 5S rDNA repeats identified a major locus in both species and a second minor locus only in C. modesta. The Southern blot and chromosome mapping data indicate the presence of different types of 5S rDNA tandem repeats in the Curimatidae genome.

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Physical Localization of 5S rRNA Genes in the Pig by Fluorescence in Situ Hybridization

Cited by 20 publications

References 13 publications

Cytogenetic studies and karyotype nomenclature of three wild canid species: maned wolf <i>(Chrysocyon brachyurus),</i> bat-eared fox <i>(Otocyon megalotis)</i> and fennec fox <i>(Fennecus zerda)</i>

Cytogenetic studies and karyotype nomenclature of three wild canid species: maned wolf <i>(Chrysocyon brachyurus),</i> bat-eared fox <i>(Otocyon megalotis)</i> and fennec fox <i>(Fennecus zerda)</i>

Dynamics of 5S rDNA in the tilapia <i>(Oreochromis niloticus)</i> genome: repeat units, inverted sequences, pseudogenes and chromosome loci

Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes)

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