Physical Localization of a Locus from Agropyron cristatum Conferring Resistance to Stripe Rust in Common Wheat

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), one of the wild relatives of wheat, exhibits resistance to stripe rust. In this study, wheat-A. cristatum 6P disomic addition line 4844-12 also exhibited resistance to stripe rust. To identify the stripe rust resistance locus from A. cristatum 6P, ten translocation lines, five deletion lines and the BC2F2 and BC3F2 populations of two wheat-A. cristatum 6P whole-arm translocation lines were tested with a mixture of two races of Pst in two sites during 2015–2016 and 2016–2017, being genotyped with genomic in situ hybridization (GISH) and molecular markers. The result indicated that the locus conferring stripe rust resistance was located on the terminal 20% of 6P short arm’s length. Twenty-nine 6P-specific sequence-tagged-site (STS) markers mapped on the resistance locus have been acquired, which will be helpful for the fine mapping of the stripe rust resistance locus. The stripe rust-resistant translocation lines were found to carry some favorable agronomic traits, which could facilitate their use in wheat improvement. Collectively, the stripe rust resistance locus from A. cristatum 6P could be a novel resistance source and the screened stripe rust-resistant materials will be valuable for wheat disease breeding.

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

“…A number of agronomically important traits were identified in Agropyron spp., including resistance to pests and diseases such as barley yellow dwarf virus (Sharma et al 1984; Shukle et al 1987), wheat streak mosaic virus (Sharma et al 1984; Brettell et al 1988; Triebe et al 1991), yellow rust, leaf rust and stem rust (Knott 1964, 1968; Cauderon and Rhind 1976; Whelan 1988; Friebe et al 1992; Zhang et al 2017), powdery mildew (Copete and Cabrera 2017) and tolerance to different abiotic stresses like cold (Limin and Fowler 1987), salinity (Dewey 1960, 1962; McGuire and Dvořák 1981; Forster et al 1987; Littlejohn 1988) and drought (Dewey 1984) and genes affecting yield (Song et al 2013). These traits would be desirable to transfer into wheat by interspecific hybridization.…”

“…Moreover, production of wheat– A. cristatum translocation lines was reported by Luan et al (2010) and Song et al (2013). In these studies, the chromosome 6P has been identified as the carrier of genes controlling yield components including the number of florets and kernels per spike (Luan et al 2010) and of a locus conferring resistance to stripe rust (Zhang et al 2017). Ochoa et al (2015) developed another line, TH4, which carries a Robertsonian translocation involving the long arm of wheat chromosome 1B and the short arm of an unidentified tetraploid A. cristatum chromosome, with substantial resistance to leaf rust.…”

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

“…As the wild‐crop relatives have not been subjected to human selection, they exhibit large genetic variation and offer a rich source of alleles and genes for crop improvement (Tanksley and McCouch, 1997). Alien gene transfer through chromosome engineering is an effective strategy to exploit this wild genetic diversity for wheat improvement (Feuillet et al, 2008; Luan et al, 2010; Molnár‐Láng et al, 2015; Ochoa et al, 2015; Zhang et al, 2017).…”

Full-length transcriptome sequences of Agropyron cristatum facilitate the prediction of putative genes for thousand-grain weight in a wheat-A. cristatum translocation line

“…Fukuhokumugi (Fukuho) and A. cristatum accession Z559 and embryo rescue [11]. Several of these lines, including additional lines, disomic substitution lines, translocation lines and introgression lines, exhibit potentially valuable traits for wheat improvement, such as disease resistance, abiotic and biotic stress tolerance and high yield and have been used in wheatbreeding programmes [12][13][14][15]. Among these lines, Pubing 3035, a Ti1AS-6PL-1AS·1AL intercalary translocation, was derived from the offspring of a wheat-A.…”