Physical map of a Plutella xylostella granulovirus genome.

Hashimoto, Yoshifumi; Hayashi, Kana; Hayakawa, Tohru; Ueno, Yasumasa; Shimojo, Ei Ichi; Kondo, Atsushi; Miyasono, Minoru; Sano, Yoshitaka; Matsumoto, Tsuguo; Granados, Robert R.

doi:10.1303/aez.2000.45

Cited by 7 publications

(2 citation statements)

References 40 publications

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“…Purification of OBs and extraction of viral DNA were carried out as described by Hashimoto et al [ 40 ], with modifications. Approximately100 infected larvae were homogenized with a blender.…”

Section: Methodsmentioning

confidence: 99%

Comparative genomic analysis of three geographical isolates from China reveals high genetic stability of Plutella xylostella granulovirus

Zhang

Wang

et al. 2021

PLoS ONE

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In this study, the genomes of three Plutella xylostella granulovirus (PlxyGV) isolates, PlxyGV-W and PlxyGV-Wn from near Wuhan and PlxyGV-B from near Beijing, China were completely sequenced and comparatively analyzed to investigate genetic stability and diversity of PlxyGV. PlxyGV-W, PlxyGV-B and PlxyGV-Wn consist of 100,941bp, 100,972bp and 100,999bp in length with G + C compositions of 40.71–40.73%, respectively, and share nucleotide sequence identities of 99.5–99.8%. The three individual isolates contain 118 putative protein-encoding ORFs in common. PlxyGV-W, PlxyGV-B and PlxyGV-Wn have ten, nineteen and six nonsynonymous intra isolate nucleotide polymorphisms (NPs) in six, fourteen and five ORFs, respectively, including homologs of five DNA replication/late expression factors and two per os infectivity factors. There are seventeen nonsynonymous inter isolate NPs in seven ORFs between PlxyGV-W and PlxyGV-B, seventy three nonsynonymous NPs in forty seven ORFs between PlxyGV-W and PlxyGV-Wn, seventy seven nonsynonymous NPs in forty six ORFs between PlxyGV-B and PlxyGV-Wn. Alignment of the genome sequences of nine PlxyGV isolates sequenced up to date shows that the sequence homogeneity between the genomes are over 99.4%, with the exception of the genome of PlxyGV-SA from South Africa, which shares a sequence identity of 98.6–98.7% with the other ones. No events of gene gain/loss or translocations were observed. These results suggest that PlxyGV genome is fairly stable in nature. In addition, the transcription start sites and polyadenylation sites of thirteen PlxyGV-specific ORFs, conserved in all PlxyGV isolates, were identified by RACE analysis using mRNAs purified from larvae infected by PlxyGV-Wn, proving the PlxyGV-specific ORFs are all genuine genes.

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Section: Methodsmentioning

confidence: 99%

Comparative genomic analysis of three geographical isolates from China reveals high genetic stability of Plutella xylostella granulovirus

Zhang

Wang

et al. 2021

PLoS ONE

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“…The purification followed the methodology proposed by Hashimoto et al (2000) with modifications, the infected larvae were macerated in autoclaved distilled water containing 1% SDS (Sodium Dodecyl Sulfate) and filtered in voile tissue to facilitate the removal of the fatty tissue of the larvae and to separate the resulting liquid from the coarser parts of the larvae. The filtrate was left under orbital stirring at 250 rpm for 30 minutes.…”

Section: Obtaining and Producing Sfmnpvmentioning

confidence: 99%

Simplified and cool texturing diets for in vivo inoculation of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)

Machado,

Dos Santos Junior,

Paiva

et al. 2023

CLCS

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The inoculation of viral entomopathogens in vivo requires study of each pathosystem. Several factors interfere in the cost of production and consequently in the availability of virus-based formulations. For the Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), employed in the management of the fall armyworm Spodoptera frugiperda, factors such as host cannibal habit, temperature, and artificial diet of viral inoculation are important to research. In this work we propose to evaluate a diet based on textured soy protein, without heating during preparation, for viral inoculation and an artificial diet of simplified composition, based on wheat germ, to complete the feeding of larvaes during the stage of viral inoculation in groups of insects. S. frugiperda larvae were inoculated with SfMNPV virus in two diets: an artificial diet similar to that of mass rearing of the host and texturized soy protein at the following temperatures and respective ages: at 25 °C at 6 and 8 days after hatching and 31 °C at 4 and 6 days after larvae hatching. And, subsequently, viral inoculation was evaluated in a group of 300 larvae under different incubation times and temperature conditions, complementing feeding at this stage with a diet of simplified composition. The results show that it is possible to inoculate viruses without loss of viral polyhedra production in a textured soy protein-based diet and supplement the larvae diet with an artificial diet of simplified composition to complete the viral infection stage in groups of infected insects.

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Current Awareness on Comparative and Functional Genomics

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Physical map of a Plutella xylostella granulovirus genome.

Cited by 7 publications

References 40 publications

Comparative genomic analysis of three geographical isolates from China reveals high genetic stability of Plutella xylostella granulovirus

Comparative genomic analysis of three geographical isolates from China reveals high genetic stability of Plutella xylostella granulovirus

Simplified and cool texturing diets for in vivo inoculation of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV)

Current Awareness on Comparative and Functional Genomics

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