Physical mapping by FISH and GISH of rDNA loci and discrimination of genomes A and B inScilla scilloides complex distributed in Korea

Choi, Hae Woon; Kim, Jeong Soon; Lee, Sang Hoon; Bang, Jae Wook

doi:10.1007/bf03036061

Cited by 17 publications

(14 citation statements)

References 30 publications

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“…Chromosome preparation FISH slides were prepared according to a previously described method (Choi et al, 2008). Briefly, the fixed root tips were washed thoroughly in distilled water prior to the enzymatic treatment.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The rRNA genes (45S and 5S) that consist of tandem repeats in the genome have been widely used as chromosomal markers in many plant species (Brown and Carlson, 1997;Taketa et al, 1999;Hasterok et al, 2001;Koo et al, 2004;Idziak and Hasterok, 2008). GISH is also a very effective method of analyzing the genetic and cytogenetic relationships between genomes in hybrids and allopolyploids (Ge and Li, 2007;Lim et al, 2007;Choi et al, 2008Choi et al, , 2009). …”

Section: Introductionmentioning

confidence: 99%

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Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Lee

Choi

Sung³

et al. 2010

Genes Genom

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Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha have long been used as Chinese medicinal herbs to treat the same conditions. To elucidate the inter-specific relationships among these species, molecular and molecular cytogenetic analyses were conducted. Cytogenetically, C. officinale and A. polymorpha were found to be diploid with 2n=2x=22, but L. chuanxiong had a triploid chromosome number (2n= 3x=33). FISH analysis revealed that each two pairs of the 45S-and 5S rRNA genes were detected on the different chromosomes of C. officinale, and that triplet of the rRNA genes were localized on the same loci of the homologue in L. chuanxiong. GISH conducted using a genomic DNA probe detected strong cross-hybridization of genomes between C. officinale and L. chuanxiong, while no distinct GISH signal between C. officinale and A. polymorpha was observed. ITS and NTS data also revealed a very high sequence homology (95−96%) between C. officinale and L. chuanxiong. The RFLP profile of the rDNA-ITS enabled us to confirm the genetic distance among species. An effective species-specific SCAR marker for the identification of the wild plant, A. polymorpha, was also developed. Taken together, the results of this study suggest two possibilities; (1) L. chuanxiong originated from C. officinale, or (2) L. Chuanxiong and C. officinale diverged from the same parental plant.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Lee

Choi

Sung³

et al. 2010

Genes Genom

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“…Simultaneous FISH of 45S and 5S rDNAs has been useful in various plants including Hordeum (Taketa et al 1999), Brassica (Snowdon et al 2000;Hasterok et al 2001), Cucumis (Koo et al 2002), Nicotiana (Lim et al 2005), and Tulipa (Mizuochi et al 2007). Genomic in situ hybridization (GISH), which uses genomic DNA as a probe, is an additional chromosome mapping technique for detecting parental genomes in allopolyploids and hybrids (Lim et al 2003a;Zhou et al 2008;Choi et al 2008Choi et al , 2009. It was proposed that triploid L. lancifolium may have originated from diploid L. lancifolium or Lilium maximowiczii, as these three species are very similar morphologically (Noda 1986).…”

Section: Introductionmentioning

confidence: 98%

Physical Mapping of rRNA Gene Loci and Inter-specific Relationships in Wild Lilium Distributed in Korea

et al. 2010

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Molecular cytogenetic analyses using fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were carried out to elucidate inter-specific relationships among wild Lilium species distributed in Korea. FISH revealed four to eight 45S rRNA gene loci, which are located on chromosomes 1-7, 10, and 11 among the different species. In contrast, the 5S rRNA gene locus was conserved on the long arm of chromosome 3, occasionally with two adjacent sites on the same chromosome arm in a few species. The 5S rDNA site was located adjacent to the 45S rDNA site in only three species, Lilium distichum, Lilium hansonii, and Lilium tsingtauense. GISH analysis using genomic DNA probes detected strong hybridization of genomes between diploid and triploid Lilium lancifolium species, demonstrating that triploid plants were derived from diploid L. lancifolium and not from Lilium maximowiczii. Phylogenetic analysis of the ITS and NTS sequences supported the cytogenetic data as well as Comber's classification of the genus Lilium.

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“…The karyotype of each genome is revealed, and their polyploids and/or hybrids between two genomes are recognized by Araki (1971) and Haga and Noda (1976). Then, four different research approaches were performed in S. scilloides such as by respective scientists for genomic constitution of population (Araki 1972a, Ding et al 1999, by cytogenetic analysis (Araki 1971), by cytogeographic studies in Japan (Ihara 1977), in Korea (Araki 1972b, 1985Choi and Bang 1990;Choi et al 2004 ), and in China (Yu and Araki 1991;Yu et al 1993;Ding et al 1998) and by molecular cytogenetic analyses (Hizume et al 1993; Araki 1994, 1996;Hizume and Shibata 2001;Choi et al 2008). Two genomes are different not only in chromosome number and karyotype, but also in genomic DNA level, revealed clearly by genomic in situ hybridization (Hizume and Araki 1996).…”

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confidence: 99%

Peculiar phenomena of rDNA locus on chromosomes of some Chinese plants in Scilla scilloides

et al. 2014

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ABSTRACT. Two molecular cytological phenomena such as NOR and/rDNA loci in the chromosome complement of Scilla scilloides were observed by in situ hybridization using rDNA probe. Most of the plants of the species collected in Shaanxi Province, the People's Republic of China were diploid of genome A that was characterized by a pair of satellited a 2 chromosomes carrying heavy rDNA signal at the secondary constriction comprising of constricted region and its adjacent chromosome arms at metaphase. At interphase the rDNA signals dispersed in the nucleolus with condensed signal body at terminal end. A plant of AA diploid showed that rDNA signal of one a 2 chromosome condensed through the cycle of respective division, whereas that of another a 2 chromosome showed the normal behavior. Another phenomenon occurred frequently in small B-chromosomes in S. scilloides. The diploid and tetraploid plants of the genome B collected in Jiangsu had several small B-chromosomes carrying condensed rDNA signal in addition to b 1 chromosomes with heavy rDNA signal at metaphase and interphase, suggesting rDNA of small B-chromosomes did not involve to nucleolus organization. Scilla scilloides is a complex species involving such two genomes as the genome A (x=8) and the genome B (x=9). The karyotype of each genome is revealed, and their polyploids and/or hybrids between two genomes are recognized by Araki (1971) and Haga and Noda (1976). Then, four different research approaches were performed in S. scilloides such as by respective scientists for genomic constitution of population (Araki 1972a, Ding et al. 1999, by cytogenetic analysis (Araki 1971), by cytogeographic studies in Japan (Ihara 1977), in Korea (Araki 1972b, 1985Choi and Bang 1990;Choi et al. 2004 ), and in China (Yu and Araki 1991;Yu et al. 1993;Ding et al. 1998) and by molecular cytogenetic analyses (Hizume et al. 1993; Araki 1994, 1996;Hizume and Shibata 2001;Choi et al. 2008). Two genomes are different not only in chromosome number and karyotype, but also in genomic DNA level, revealed clearly by genomic in situ hybridization (Hizume and Araki 1996). Then, S. scilloides is useful to study genome differentiation, because they have differences between A and B genomes in genome structure/karyotype and their genomic DNA differentiation. In the present study two phenomena concerning rDNA loci/NORs by in situ hybridization (ISH) using rDNA probe are described for the first time. Ding et al. (1998). Plants were raveled and cultivated in pots and maintained in the experimental garden of Ehime University. Root tips were collected and treated in 2mM 8-hydroxiquinoline for 3h. Then, they were fixed in acetic-ethanol (1:3, volume ratio) and used immediately for observation or stored in a deep freezer until use. KEYWORDS: MATERIALS AND METHODS Thirty plants of Scilla scilloidesThe fixed root tips were soaked in 45% acetic acid for 5 min, treated in 45% acetic acid at 60°C for 8 min and transferred in cold 45% acetic acid. Then, they were squashed in 45% acetic acid under cover slips on...

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Physical mapping by FISH and GISH of rDNA loci and discrimination of genomes A and B inScilla scilloides complex distributed in Korea

Cited by 17 publications

References 30 publications

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Physical Mapping of rRNA Gene Loci and Inter-specific Relationships in Wild Lilium Distributed in Korea

Peculiar phenomena of rDNA locus on chromosomes of some Chinese plants in Scilla scilloides

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