Physical mapping of 18S rDNA cistron in species of the Omophoita genus (Coleoptera, Alticinae) using fluorescent in situ hybridization

Almeida, Mara Cristina de; Goll, Leonardo Gusso; Artoni, Roberto Ferreira; Nogaroto, Viviane; Matiello, Rodrigo Rodrigues; Vicari, Marcelo Ricardo

doi:10.1016/j.micron.2010.06.008

Cited by 24 publications

(33 citation statements)

References 35 publications

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“…The FISH was performed following the technique of Pinkel et al (1986) with modifi cations described by Almeida et al (2010). However, before the FISH procedure, the chromosome preparations were incubated overnight in 70% acetic acid solution.…”

Section: Methodsmentioning

confidence: 99%

“…The images of the chromosomes were taken using a Zeiss Imager A2 equipped with an Axio Cam digital camera, using Axion Vision software (Carl Zeiss AG, Jena, Germany). Silva et al, 2009;Almeida et al, 2010;Cabral de Mello et al, 2010, 2011aMendes-Neto et al, 2010;Oliveira et al, 2010Oliveira et al, , 2012aGiannoulis et al, 2011;Proença et al, 2011;Lira-Neto et al, 2012;Karagyan et al, 2012;Goll et al, 2013Goll et al, , 2015.…”

Section: Methodsmentioning

confidence: 99%

“…Among the 17 species for which the number of NORs is determined, 14 have one site located on one autosomal pair, two species have two pairs of autosomes with NORs, and one species has only one NOR located on the neoX chromosome (Petitpierre, 1970(Petitpierre, , 1976(Petitpierre, , 1996Virkki, 1983;Virkki & Denton, 1987;Postiglioni et al, 1990Postiglioni et al, , 1991Yadav et al, 1992;Schneider et al, 2002;Gómez-Zurita et al, 2004;Almeida et al, 2006Almeida et al, , 2010.…”

Section: Methodsmentioning

confidence: 99%

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Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae)

Lopes¹,

Fernandes²,

Schneider³

2017

Eur. J. Entomol.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae)

Lopes¹,

Fernandes²,

Schneider³

2017

Eur. J. Entomol.

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“…Small differences could be observed through the alignments, but this similarity confirms the nature of the isolated sequence. Almeida et al (2010) analyzed the 18S rDNA isolated sequence of Omophoita octoguttata (Fabricius, 1875) (Coleoptera) and also found these differences in these kinds of repetitive DNA. Those authors attributed such differences to the result of transition and transversion base changes.…”

Section: Discussionmentioning

confidence: 99%

“…In Hemiptera, these studies have revealed important findings compared to those obtained in other groups of insects (Caterino et al, 2000). Besides 18S rDNA, other classes of repetitive DNA with heterochromatic sequences have also been widely used as cytogenetic markers for comparisons across different groups of insects such as in Orthoptera (Cabral-de-Mello et al, 2011), Coleoptera (Almeida et al, 2010), Diptera (Rafael et al, 2006), and Heteroptera Bardella et al, 2010;Grozeva et al, 2010;Panzera et al, 2012). Some types of repetitive DNA can be chromosomespecific, such as those mostly found in Drosophila melanogaster (Bonaccorsi and Lohe, 1991) and those found on the sex chromosomes in species such as Megoura (Bizzaro et al, 1996).…”

Section: Introductionmentioning

confidence: 96%

Physical mapping of 18S rDNA and heterochromatin in species of family Lygaeidae (Hemiptera: Heteroptera)

Bardella¹,

Sampaio²,

Venturelli³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Analyses conducted using repetitive DNAs have contributed to better understanding the chromosome structure and evolution of several species of insects. There are few data on the organization, localization, and evolutionary behavior of repetitive DNA in the family Lygaeidae, especially in Brazilian species. To elucidate the physical mapping and evolutionary events that involve these sequences, we cytogenetically analyzed three species of Lygaeidae and found 2n (♂) = 18 (16 + XY) for Oncopeltus femoralis; 2n (♂) = 14 (12 + XY) for Ochrimnus sagax; and 2n (♂) = 12 (10 + XY) for Lygaeus peruvianus. Each species showed different quantities of heterochromatin, which also showed variation in their molecular composition by fluorochrome Physical mapping in Lygaeidae staining. Amplification of the 18S rDNA generated a fragment of approximately 787 bp. The alignment of the consensus sequence with sequences from other species of Heteroptera deposited in the GenBank revealed a similarity of 98% with small differences. Fluorescent in situ hybridization with the 18S rDNA fragment revealed that this ribosomal gene was located in 1 autosomal pair at different positions in the three species. No cytogenetic data are available for these Brazilian species. The basal number and the possible chromosomal changes that occurred among the different species, as well as the evolution of these DNA sequences, are discussed.

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Mechanisms of karyotype evolution in the Brazilian scorpions of the subfamily Centruroidinae (Buthidae)

2018

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The recently-revised subfamily Centruroidinae is part of the New World clade of buthid scorpions. In this study, we analyzed the cytogenetic characteristics of nine of the 10 Brazilian centruroidines, and one undescribed species of the genus Ischnotelson, using a phylogenetic approach to determine the chromosomal rearrangements responsible for the differentiation of karyotypes among the species. The cytogenetic data recorded in the present study supported the new taxonomic arrangement of the Centruroidinae, with all the species of the same genus sharing the same or similar diploid numbers, i.e., 2n = 20 or 22 in Troglorhopalurus lacrau and T. translucidus, 2n = 25 or 26 in Ischnotelson sp., I. guanambiensis and I. peruassu, and 2n = 28 in Jaguajir agamemnon, J. pintoi and J. rochae. The karyotype modelling in the ChromEvol software indicated 2n = 18 as the ancestral diploid number of the Centruroidinae. The differentiation of karyotypes among the centruroidine genera was based on increasing chromosome numbers resulting from progressive fission events. These changes probably occurred prior to the diversification of the genera Ischnotelson, Jaguajir, Physoctonus and Rhopalurus, and appear to have played a more important role in karyotype evolution at the intergeneric level than the interspecific one. However, the observed increase in diploid numbers was not accompanied by changes in the number or location of ribosomal genes or telomeric sequences. The identification of meiotic cells in female specimens also allowed us to discuss the mechanisms of achiasmatic meiosis in scorpions.

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Physical mapping of 18S rDNA cistron in species of the Omophoita genus (Coleoptera, Alticinae) using fluorescent in situ hybridization

Cited by 24 publications

References 35 publications

Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae)

Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae)

Physical mapping of 18S rDNA and heterochromatin in species of family Lygaeidae (Hemiptera: Heteroptera)

Mechanisms of karyotype evolution in the Brazilian scorpions of the subfamily Centruroidinae (Buthidae)

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