At present, the instability of floor roadway is increasingly serious, and even the representative high-performance U-steel support cannot provide roadways with completely effective support. Thus, the mechanism of roadway deformation should be analyzed rather than only considering the roadway support. To study the influence of coal mining on the stability of floor roadways under tectonic stress, taking the floor roadway under No.8 coal seam in Luling Coal Mine in China as the research object, the roadway deformation was tested with and without tectonic stress through the similar physical simulation method. Finally, the changing mechanisms of the stress field, fracture development and displacement field of the surrounding rocks in the floor roadway under mining effects were systematically analyzed. The results show that: (1) under the same stress condition, deformation and fracture of the floor roadway increase with the increase of mining step; (2) without tectonic stress, the maximum hoop stress of the floor roadway arch foot is 32.5 MPa located 2.7 m away from the roadway wall. Meanwhile, under tectonic stress, the maximum stress changes to 38.5 MPa at 3.6 m away from the roadway wall; (3) whether under mining effects or not, the deformation time is longer and deformation failure is larger under tectonic stress. The conclusions indicate the tectonic stress field has significant on roadway deformation and failure, which can provide the technical reference for the optimization of mine roadway layout.