Physical models of bacteriophage lytic enzymes: Sidwell Friends School S.M.A.R.T. Team Project

Abstract

The Sidwell Friends School S.M.A.R.T. Team (Students Modeling A Research Topic) has been working with the Nelson laboratory at the University of Maryland to design and produce accurate, three‐dimensional physical models of bacteriophage lysins using rapid prototyping (RP) technology. Lysins are bacterial cell wall hydrolases used by bacteriophage during progeny release. These enzymes in their pure form can be applied directly to Gram positive bacteria to quickly induce hypotonic lysis from without. This rapid activity shows promise as a novel antibacterial agent to both prevent and treat infections. Discussions with members of the Nelson laboratory allowed the students to design models of lysins to highlight their structural and functional characteristics. These designs were used to direct RP machines to build physical models of these enzymes. These physical models serve as “communication tools” used to enhance the understanding of lysins and their applications among the scientific and academic community. By contributing this tool to the Nelson laboratory research team, the students have the unique opportunity to experience and participate in the activities of a research laboratory. This work is supported by a grant awarded to Tim Herman by the NIH NCRR SEPA program and the HHMI Precollege Science Education Program.