The environmental constrains issued recently in many countries reinforce the textile chemists and colorists to adopt eco-friendlier methods during dyeing and finishing of textile substrates. Herein, a genuine colorant extracted from mulberry leaves was used in dyeing of natural silk fabric using conventional heating as well as ultrasonic-or microwave-assisted dyeing. Dyeing of silk fabric was conducted also in presence of iron II sulphate heptahydrate as a mordant using different methods of mordanting. The colour strength, colorimetric data, fastness properties, antimicrobial properties, and the resistance to ultraviolet radiation of the dyed samples were monitored. The results proved that mulberry leaves' extract is a suitable eco-friendlier colorant for dyeing of natural silk fabrics with a high colour strength and satisfactory fastness towards light, washing, perspiration and crocking, in presence of FeSO4.7H2O as a mordant. The dyed fabrics exhibited excellent resistance to microbial attack, including Gram +ve, Gram -ve bacteria and a pathogenic fungus, as well as ultraviolet radiation.