Recently, researchers have drawn their attention to industrial hemp ( Canabis sativa L.) and stinging nettle ( Urtica dioica L.), as feedstocks, potentially having a wide nonfood application. The aim of the present work was to compare dry matter (DM) and carbon (C) yields as well as C concentration in the above-ground biomass, stems and shives of the mentioned crops. In this chapter, extra attention has been paid to the C accumulation in stems and shives, since stems are a more environmentally friendly resource for solid biofuel compared to the whole aboveground part of the plant, and shives are an agricultural waste.Field experiments with industrial hemp (eight varieties) and stinging nettle (one wild nettle and two treatments of fibre nettle clone) were carried out during 2010-2012. Dew retting and water retting were used to extract the fibre. C concentration in the samples of hemp and nettle was determined by wet oxidation with dichromate.DM yield of the above-ground biomass of hemp amounted to an average of 10607 kg ha − 1 , of stems 9063 kg ha − 1 with high C concentrations of 555 and 568 g kg − 1 DM, respectively. DM yield of the nettle declined along with a harvest year and ranged from 11604 kg ha − 1 (2010) to 5596 kg ha − 1 (2012) averaging