Physical performance of exercising young rats fed hydrolysed whey protein at a sub-optimal level

Pimenta, Fernanda Motta Veiga; Abecia-Soria, María Inés; Auler, Flavia; Amaya-Farfán, Jaime

doi:10.1016/j.idairyj.2005.12.006

Cited by 23 publications

(43 citation statements)

References 16 publications

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Section: T H Meek and Otherssupporting

confidence: 86%

“…6). Exhaustive treadmill exercise further increased blood lactate concentrations of both HR and C lines, to values consistent with the literature for laboratory house mice (Pederson et al, 2005a;Pimenta et al, 2006; Hakimi et al, 2007), with these values being somewhat lower than reported for wild rodents of other species (Djawdan, 1993).Exercise is considered a key component of body weight regulation and a cornerstone for the treatment of metabolic syndrome and other metabolic disorders (Thorburn and Proietto, 2000). As worldwide rates of obesity [and associated mortality (e.g.…”

supporting

confidence: 78%

“…females have evolved higher running distances almost exclusively by increased running speed, whereas male HR mice show a significant increase in the amount of time spent running in addition to average running speed (Garland, 2003) [ Rezende et al (Rezende et al, 2009) and references therein]. The exercise-physiological underpinnings of this sex difference in response to selective breeding are not yet known, but a recently discovered sex difference in the response to an endocannabinoid receptor antagonist points to a 'motivational' difference between HR males and females (Keeney et al, 2008).Resting blood glucose and lactate values of both HR and C mice (Figs 4-6) are generally consistent with other studies of mice (Tsuboyama-Kasaoka et al, 1999;Banerjee et al, 2004;Pederson et al, 2005a;Pederson et al, 2005b; Ferreira et al, 2007;Svenson et al, 2007;Wende et al, 2007), rats (Pimenta et al, 2006), hamsters (Mistlberger et al, 2006) and human beings (Goldfarb et al, 1986;Rizzo et al, 2005;Scheen et al, 1998), although the lactate values are slightly higher than in some of the previous mouse studies, possibly because our study is apparently the first to measure lactate in samples obtained from the submandibular vein using lancets. The observed higher resting glucose concentrations in males are also consistent with previous studies of mice (Pederson et al, 2005a;Pederson et al, 2005b;Svenson et al, 2007).…”

supporting

confidence: 76%

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Endurance capacity of mice selectively bred for high voluntary wheel running

Meek

Lonquich

Hannon

et al. 2009

Journal of Experimental Biology

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SUMMARYMice from four lines bred for high voluntary wheel activity run ~3-fold more revolutions per day and have elevated maximal oxygen consumption during forced treadmill exercise, as compared with four unselected control (C) lines. We hypothesized that these high runner (HR) lines would have greater treadmill endurance-running capacity. Ninety-six mice from generation 49 were familiarized with running on a motorized treadmill for 3 days. On days 4 and 5, mice were given an incremental speed test (starting at 20 m min -1 , increased 1.5 m min -1 every 2 min) and endurance was measured as the total time or distance run to exhaustion. Blood samples were taken to measure glucose and lactate concentrations at rest during the photophase, during peak nightly wheel running, and immediately following the second endurance test. Individual differences in endurance time were highly repeatable between days (r=0.79), and mice tended to run longer on the second day (paired t-test, P<0.0001). Blood glucose following the treadmill test was low for all animals (~53 mg dl -1 ) and lactate was high (~6.5 mmol l -1 ), suggesting that exhaustion occurred. The HR lines had significantly higher endurance than the C lines (1-tailed P<0.05), whether or not body mass was used as a covariate in the analysis. The relationship between line means for wheel running and treadmill endurance differed between the sexes, reinforcing previous studies that indicate sex-specific responses to selective breeding. HR mice appear to have a higher endurance capacity than reported in the literature for inbred strains of mice or transgenics intended to enhance endurance.Key words: artificial selection, endurance, exhaustion, experimental evolution, genetics, glucose, lactate, locomotor activity, wheel running. THE JOURNAL OF EXPERIMENTAL BIOLOGY THE JOURNAL OF EXPERIMENTAL BIOLOGY 2909Endurance capacity of high-activity mice Kelly et al., 2006), increased insulin-stimulated glucose uptake by isolated extensor digitorum longus muscle (Dumke et al., 2001), and more GLUT4 in the gastrocnemius after 5 days of wheel access (Gomes et al., 2009). In addition, blood haemoglobin concentration was a positive predictor of V O2max in the HR lines, but a negative predictor in the C lines (Rezende et al., 2006c). However, direct measurements of endurance-running ability have not yet been performed.The purpose of the present study was to test the hypothesis that HR mice have greater endurance than C mice in a graded-speed treadmill test (e.g. Lerman et al., 2002; Haubold et al., 2003). We also measured blood glucose and lactate concentrations at rest, during voluntary wheel running and at the end of the endurance test. We hypothesized that wheel running would increase blood lactate concentrations relative to those at rest, and that concentrations after the endurance test would be even higher. We also hypothesized that blood glucose concentrations would be decreased following the endurance trial compared with values at rest. In human beings, endurance-trained subjects e...

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Section: T H Meek and Otherssupporting

confidence: 86%

supporting

confidence: 78%

supporting

confidence: 76%

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Endurance capacity of mice selectively bred for high voluntary wheel running

Meek

Lonquich

Hannon

et al. 2009

Journal of Experimental Biology

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“…The various advantages pointed out for the whey protein hydrolyzates are consistent also with a possible beneficial effect to the experimental animal, as has been the case of physical performance in rats undergoing endurance exercise (Morifuji et al, 2009;Pimenta et al, 2005) and promoting the synthesis of glycogen (Morifuji et al, 2009). Nery-Diez et al (2010 additionally have suggested an anti-stress effect associated with the consumption of the whey protein hydrolyzate in rats undergoing endurance exercise.…”

Section: Introductionsupporting

confidence: 58%

“…The weights of the gastrocnemius muscles also did not differ between groups. In the study of Pimenta et al (2005), no significant changes in muscle protein content were also observed when comparing the whey protein isolate with the hydrolyzate (medium degree of hydrolysis ~8.5 -9.0 %) in the diets of exercising young rats. Taken together, the results of this experiment suggest that the milk whey proteins, whether intact or prehydrolyzed, whether associated or not with physical endurance activity, did not result in an increase of the contents of muscle protein.…”

Section: Resultsmentioning

confidence: 91%

Prehydrolyzed dietary protein reduces gastrocnemial DNA without impairing physical capacity in the rat

Zaffani¹,

Carneiro-Leão²,

Costa³

et al. 2013

Afr. J. Biotechnol.

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Previous studies have shown that hydrolyzed proteins exhibit antioxidant properties and may confer physical and physiological advantages when consumed by the exercising rat. The purpose of this study was to compare the effects of feeding either intact (I) and partly hydrolyzed (H) milk whey proteins on gastrocnemial DNA contents and protein metabolism in exercising Wistar rats. Protein synthesis and degradation, protein and DNA contents, and concentration of the serum insulin-like growth factor-1 (IGF1) were determined in six experimental groups according to the type of protein consumed [casein (C), whey protein isolate (I), hydrolyzed whey protein (H) and level of physical activity (sedentary (S) and trained (T)]. H produced significantly lower rates of protein synthesis and degradation and DNA contents in the gastrocnemius, while no differences were observed in the total muscle protein content and serum levels of IGF1. These results indicate that consumption of prehydrolyzed whey protein alters muscle metabolism resulting in less DNA, but maintains the muscle protein levels constant and sustain or improves physical performance, compared to the unhydrolyzed protein.

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