Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Hsu, Miao Hsing; Chen, Szu-Han; Tseng, Wan‐Ling; Hung, Kuo-Shu; Chung, Tzu-Chun; Lin, Shio‐Jean; Koo, Jahyun; Hsueh, Yuan Yu

doi:10.3389/fbioe.2023.1217067

Cited by 7 publications

(2 citation statements)

References 56 publications

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“…This research is crucial for advancing the treatment of severe nerve injuries where traditional methods may not be sufficient ( Garcia-Garcia et al, 2023 ). Decellularized nerve grafts should provide a suitable substrate for nerve regeneration in which the conservation of ECM components plays a crucial role for peripheral nerve regeneration and axonal guidance ( Hsu et al, 2023 ; Mahdian et al, 2023 ). The currently available decellularized methods are time and effort consuming ( El Soury et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Muratori,

Crosio,

Ronchi

et al. 2024

Front. Neuroanat.

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IntroductionPeripheral nerves are frequently affected by lesions caused by traumatic or iatrogenic damages, resulting in loss of motor and sensory function, crucial in orthopedic outcomes and with a significant impact on patients’ quality of life. Many strategies have been proposed over years to repair nerve injuries with substance loss, to achieve musculoskeletal reinnervation and functional recovery. Allograft have been tested as an alternative to the gold standard, the autograft technique, but nerves from donors frequently cause immunogenic response. For this reason, several studies are focusing to find the best way to decellularize nerves preserving either the extracellular matrix, either the basal lamina, as the key elements used by Schwann cells and axons during the regenerative process.MethodsThis study focuses on a novel decellularization protocol for porcine nerves, aimed at reducing immunogenicity while preserving essential elements like the extracellular matrix and basal lamina, vital for nerve regeneration. To investigate the efficacy of the decellularization protocol to remove immunogenic cellular components of the nerve tissue and to preserve the basal lamina and extracellular matrix, morphological analysis was performed through Masson’s Trichrome staining, immunofluorescence, high resolution light microscopy and transmission electron microscopy. Decellularized porcine nerve graft were then employed in vivo to repair a rat median nerve lesion. Morphological analysis was also used to study the ability of the porcine decellularized graft to support the nerve regeneration.Results and DiscussionThe decellularization method was effective in preparing porcine superficial peroneal nerves for grafting as evidenced by the removal of immunogenic components and preservation of the ECM. Morphological analysis demonstrated that four weeks after injury, regenerating fibers colonized the graft suggesting a promising use to repair severe nerve lesions. The idea of using a porcine nerve graft arises from a translational perspective. This approach offers a promising direction in the orthopedic field for nerve repair, especially in severe cases where conventional methods are limited.

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Section: Discussionmentioning

confidence: 99%

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Muratori,

Crosio,

Ronchi

et al. 2024

Front. Neuroanat.

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“…This review article specifically focuses on nerve regeneration using decellularized nerve tissues (DNT). In recent studies, the use of biological scaffolds has been the focus of researchers in nerve repair ( Hsu et al, 2023 ; Yu et al, 2023 ). Therefore, we decided to mention this issue in this paper, and we hope that we have helped those interested in this field.…”

Section: Introductionmentioning

confidence: 99%

Nerve regeneration using decellularized tissues: challenges and opportunities

Mahdian,

Tabatabai,

Abpeikar

et al. 2023

Front. Neurosci.

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In tissue engineering, the decellularization of organs and tissues as a biological scaffold plays a critical role in the repair of neurodegenerative diseases. Various protocols for cell removal can distinguish the effects of treatment ability, tissue structure, and extracellular matrix (ECM) ability. Despite considerable progress in nerve regeneration and functional recovery, the slow regeneration and recovery potential of the central nervous system (CNS) remains a challenge. The success of neural tissue engineering is primarily influenced by composition, microstructure, and mechanical properties. The primary objective of restorative techniques is to guide existing axons properly toward the distal end of the damaged nerve and the target organs. However, due to the limitations of nerve autografts, researchers are seeking alternative methods with high therapeutic efficiency and without the limitations of autograft transplantation. Decellularization scaffolds, due to their lack of immunogenicity and the preservation of essential factors in the ECM and high angiogenic ability, provide a suitable three-dimensional (3D) substrate for the adhesion and growth of axons being repaired toward the target organs. This study focuses on mentioning the types of scaffolds used in nerve regeneration, and the methods of tissue decellularization, and specifically explores the use of decellularized nerve tissues (DNT) for nerve transplantation.

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Exploiting the Potential of Decellularized Extracellular Matrix (ECM) in Tissue Engineering: A Review Study

Brouki Milan,

Masoumi,

Biazar

et al. 2024

Macromolecular Bioscience

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While significant progress has been made in creating polymeric structures for tissue engineering, the therapeutic application of these scaffolds remains challenging owing to the intricate nature of replicating the conditions of native organs and tissues. The use of human‐derived biomaterials for therapeutic purposes closely imitates the properties of natural tissue, thereby assisting in tissue regeneration. Decellularized extracellular matrix (dECM) scaffolds derived from natural tissues have become popular because of their unique biomimetic properties. These dECM scaffolds can enhance the body's ability to heal itself or be used to generate new tissues for restoration, expanding beyond traditional tissue transfers and transplants. Enhanced knowledge of how ECM scaffold materials affect the microenvironment at the injury site is expected to improve clinical outcomes. In this review, recent advancements in dECM scaffolds are explored and relevant perspectives are offered, highlighting the development and application of these scaffolds in tissue engineering for various organs, such as the skin, nerve, bone, heart, liver, lung, and kidney.

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Physical processing for decellularized nerve xenograft in peripheral nerve regeneration

Cited by 7 publications

References 56 publications

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Exploring an innovative decellularization protocol for porcine nerve grafts: a translational approach to peripheral nerve repair

Nerve regeneration using decellularized tissues: challenges and opportunities

Exploiting the Potential of Decellularized Extracellular Matrix (ECM) in Tissue Engineering: A Review Study

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