Flaxseed meal protein (FMP) films were prepared, and their mechanical properties (tensile strength (TS) and elongation at break (E) values), water vapour permeability, optical properties and thermogravimetric analysis were evaluated. Briefly, 5 g FMP, 2 g fructose and 0.03 g ferulic acid were required for the optimal preparation of an FMP film. The TS and E values of the FMP film were 13.12 MPa and 61.90%, respectively. Furthermore, different amounts of lemongrass oil (LE) were incorporated into the FMP film to prepare an antimicrobial film. Wrapping pen shell adductor muscle with the FMP film containing 1.0% LE reduced the counts of inoculated Listeria monocytogenes and Escherichia coli O157:H7 compared with those in the control, after storage at 4°C for 12 day. Consequently, packaging with FMP film containing 1.0% LE can be useful in improving the quality of pen shell adductor muscle during storage.