Nanotechnology (NT) and nanoparticles (NPs) have left a huge impact on every field of science today, but they have shown tremendous importance in the fields of cosmetics and environmental cleanup. NPs with photocatalytic effects have shown positive responses in wastewater treatment, cosmetics, and the biomedical field. The chemically synthesized TiO2 nanoparticles (TiO2 NPs) utilize hazardous chemicals to obtain the desired-shaped TiO2. So, microbial-based synthesis of TiO2 NPs has gained popularity due to its eco-friendly nature, biocompatibility, etc. Being NPs, TiO2 NPs have a high surface area-to-volume ratio in addition to their photocatalytic degradation nature. In the present review, the authors have emphasized the microbial (algae, bacterial, fungi, and virus-mediated) synthesis of TiO2 NPs. Furthermore, authors have exhibited the importance of TiO2 NPs in the food sector, automobile, aerospace, medical, and environmental cleanup.