Physical Studies on Corneal Tissues

Adler, H. J.; Gross, S. T.; Lambert, J. M.

doi:10.1126/science.109.2833.383-a

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…Hertel froze his specimens and cooled them to liquid air temperatures so that artifacts resulting from the crystallization of water are possible, and the structural information is probably not relevant to natural hydration. Adler et al (1949) outlined results which apparently are in conformity with those of Hertel, but they published little detail of their findings. Agarwal et al (1972) examined dry cornea, but were unable to study fresh tissue because of their instrumental setup.…”

Section: Introductionmentioning

confidence: 67%

Synchrotron x-ray diffraction studies of the cornea, with implications for stromal hydration

Meek¹,

Fullwood²,

Cooke³

et al. 1991

Biophysical Journal

108

113

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The intermolecular and interfibrillar spacings of collagen in bovine corneal stroma have been measured as a function of tissue hydration. Data were recorded from low- and high-angle x-ray diffraction patterns obtained using a high intensity synchrotron source. The most frequently occurring interfibrillar spacing varied from 34 nm in dry corneas to 76 nm at H = 5 (the hydration, H, is defined as the ratio of the weight of water to the dry weight). The most frequently occurring intermolecular Bragg spacing increased from 1.15 nm (dry) to approximately 1.60 nm at normal hydration (H approximately 3.2) and continued to increase only slowly above normal hydration. Most of the increase in the intermolecular spacing occurred between H = O and H = 1. Over this hydration range the interfibrillar and intermolecular spacings moved in tandem, which suggests that the initial water goes equally within and between the fibrils. Above H = 1 water goes preferentially between the fibrils. The results suggest that, even at normal hydration, water does not fill the interfibrillar space uniformly, and a proportion is located in another space or compartment. In dried-then-rehydrated corneas, a larger proportion of the water goes into this other compartment. In both cases, it is possible to postulate a second set or population of fibrils that are more widely and irregularly separated and therefore do not contribute significantly to the diffraction pattern.

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Section: Introductionmentioning

confidence: 67%

Synchrotron x-ray diffraction studies of the cornea, with implications for stromal hydration

Meek¹,

Fullwood²,

Cooke³

et al. 1991

Biophysical Journal

108

113

View full text Add to dashboard Cite

show abstract

“…2 The patterns from X-ray diffraction analyses of cat corneas partly provided the basis for this theory, although other older X-ray studies also tried to assess whether these patterns could change according to the water content (hydration) of the corneal tissue. 30,31 Highly focussed X-ray sources have been used to not only generate higher resolution diffraction patterns but ones from which some type of measurement of an overall average spacing between the collagen fibrils could be made. [32][33][34] These later studies also indicate that the inter-fibril spacing in the corneal stroma can be expected to change in relation to the hydration of the tissue sample.…”

Section: Discussionmentioning

confidence: 99%