Physical Therapy and Rehabilitation

Schmitt‐Sody, Marcus

doi:10.1007/978-3-319-20300-3_20

Cited by 1 publication

(3 citation statements)

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“…Liposomes are among the most investigated synthetic carriers of cytotoxic hydrophobic drugs in cancer therapeutics worldwide [22][23][24][25][26][27][28][29][30] . Lipid NPs are suitable drug delivery vehicles for hydrophobic drugs and decrease side effects compared to established solvent systems (such as Cremophor EL in Taxol) 14,31,32 .…”

Section: Introductionmentioning

confidence: 99%

“…Of the different types of lipid NPs, cationic liposome (CL) NPs were chosen for this study because positively charged particles have been shown to passively accumulate in tumors 30,[40][41][42][43][44] . Tumor vasculature has a greater negative charge than other tissue 26,42 , and thus positively charged particles adhere more to this area.…”

Section: Introductionmentioning

confidence: 99%

“…We used confocal microscopy, flow cytometry, and cryogenic TEM to uncover the key differences underlying the interactions of bare and PEG-CL ptx NPs with cells that cause PEGylation to enhance PTX delivery. Cryo-TEM shows that adding PEG-lipid (5 and 10 mol% PEG2K-lipid) or charged lipid (30,50 and 80 mol% DOTAP) to neutral DOPC vesicles induces a structural transition to a mixture of nanometer-scale vesicles and anisotropic micelles (primarily disc-shaped bicelles, Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

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PEGylation of Paclitaxel-Loaded Cationic Liposomes Drives Steric Stabilization of Bicelles and Vesicles thereby Enhancing Delivery and Cytotoxicity to Human Cancer Cells

Steffes¹,

Zhang

MacDonald³

et al. 2019

ACS Appl. Mater. Interfaces

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Polyethylene glycol (PEG) is a polymer used widely in drug delivery to create "stealth" nanoparticles (NPs); PEG coatings suppress NP detection and clearance by the immune system and beneficially increase NP circulation time in vivo. However, NP PEGylation typically obstructs cell attachment and uptake in vitro compared to the uncoated equivalent. Here, we report on a cationic liposome (CL) NP system loaded with the hydrophobic cancer drug paclitaxel (PTX) in which PEGylation (i.e. PEG-CL ptx NPs) unexpectedly enhances, rather than diminishes, delivery efficacy and cytotoxicity to human cancer cells. This highly unexpected enhancement occurs even when the PEG-chains coating the NP are in the transition regime between the mushroom and brush conformations. Cryogenic TEM of PEG-CL ptx NPs shows that PEG causes the proliferation of a mixture of sterically stabilized nanometer-scale vesicles and anisotropic micelles (e.g. bicelles). Remarkably, the onset of bicelles at sub-monolayer concentrations of the PEG-coat has to our knowledge not been previously reported; it was previously thought that PEG-lipid in this

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