Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Murashige and Skoog Plant Cell Culture Media

Abstract

Abstract:The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. The study was performed in two groups; one was kept as control while another was subjected to Mr. Trivedi's biofield energy treatment and coded as treated group. Afterward, both the control and treated samples were analyzed using various analytical techniques. The X-ray diffraction (XRD) analysis showed 19.92% decrease in the crystallite size of treated sample with respect to the control. The thermogravimetric analysis (TGA) showed the increase in onset temperature of thermal degradation (T onset ) by 9.41% and 10.69% in first and second steps of thermal degradation, respectively after the biofield energy treatment as compared to the control. Likewise, T max (maximum thermal degradation temperature) was increased by 17.43% and 28.61% correspondingly in the first and second step of thermal degradation in the treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis indicated the 143.51% increase in the latent heat of fusion of the treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated MS media showed the alteration in the frequency such as 3165→3130 cm -1 (aromatic C-H stretching); 2813→2775 cm -1 (aliphatic C-H stretching); 1145→1137 cm -1 (C-N stretching), 995→1001 cm -1 (S=O stretching), etc. in the treated sample with respect to the control. The UV spectra of control and treated MS media showed the similar absorbance maxima (λ max ) i.e. at 201 and 198 nm, respectively. The XRD, TGA-DTG, DSC, and FT-IR results suggested that Mr. Trivedi's biofield energy treatment has the impact on physical, thermal, and spectral properties of the MS media. As a result, the treated MS media could be more stable than the control, and might be used as better media in the plant tissue culture technique.

Evaluation of Isotopic Abundance Ratio in Biofield Energy Treated Nitrophenol Derivatives Using Gas Chromatography-Mass Spectrometry

“…the isotopic abundance ratio of P M+1 /P M ( 13 C/ 12 C or 2 H/ 1 H or 15 N/ 14 N) in 4-bromoaniline was increased after biofield energy treatment up to 368.3% [18]. The isotopic abundance ratio of P M+2 /P M ( 18 O/ 16 O or 37 Cl/ 35 Cl) in biofield treated 2,4-dichlorophenol was increased by 40.57%, respectively [20]. Biofield energy is an electromagnetic field existed in an around the human body [21][22][23].…”

Evaluation of the Impact of Biofield Energy Healing Treatment (The Trivedi Effect<sup>®</sup>) on the Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate

“…Biofield Energy Healing Treatment (The Trivedi Effect ® ) has been published in numerous peerreviewed science journals due to its significant impacts in the science fields of biotechnology, genetics, cancer, microbiology, materials science, agriculture, and pharmaceuticals. These publications reported that Biofield Energy Treatment (The Trivedi Effect ® ) has the astounding capability to transform the physical, structural, chemical, thermal and behavioral properties of several pharmaceuticals [23][24][25], nutraceuticals [26,27], organic compounds [28][29][30], metals and ceramics in materials science [31][32][33], improve the overall productivity of crops [34][35][36], as well as modulate the efficacy of various living cells [37][38][39][40]. Although magnesium gluconate exhibits the highest bioavailability and moderate solubility in water in comparison to other magnesium salts, humans still face problems in achieving their daily requirements of magnesium [41].…”

Physicochemical, Thermal, Structural, and Behavioral Properties Analysis in Magnesium Gluconate: An Effect of the Energy of Consciousness (The Trivedi Effect<sup>®</sup>)

“…The Biofield Energy Healing Treatment (The Trivedi Effect ® ) has been published in numerous peerreviewed science journals due to its significant impacts in the science fields of biotechnology, genetics, cancer, microbiology, materials science, agriculture, and pharmaceuticals. These publications reported that the Biofield Energy Treatment (The Trivedi Effect ® ) has the astounding capability to transform the physical, structural, chemical, thermal and behavioral properties of several pharmaceuticals [23][24][25], nutraceuticals [26,27], organic compounds [28][29][30], metals and ceramic in materials science [31][32][33], and improve the overall productivity of crops [34][35][36] as well as to modulate the efficacy of the various living cells [37][38][39][40]. Although magnesium gluconate exhibits the highest bioavailability and moderate solubility in water in comparison to other magnesium salts, humans still face problems in achieving their daily requirements of magnesium [41].…”