Physico-chemical and morphological aspects of temperate phage α particles

Aurisicchio, S.; Chiozzotto, A.; Coppo, Anna; Donini, Pierluigi; Frontali, C.; Graziosi, Francesca

doi:10.1007/bf02783542

Cited by 10 publications

(1 citation statement)

References 7 publications

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“…DNA-dependent RNA polymerase assay. Reactions were carried out in a 1.0-ml mixture containing the following components: Tris buffer (pH 7.9), 40 ,Amoles; ,B-mercaptoethanol, 12 jmoles; MnSO4 H20, 1 ,umole; MgCI2 * H20, 4 umoles; adenosine, guanosine, uridine, and cytidine S'-triphosphates, 0.1 ,Amole each; pyruvate kinase (Calbiochem, A grade), 40 ,ug; phosphoenolpyruvate (Calbiochem, A grade), 4 ,Amoles; calf thymus DNA (Worthington Biochemical Corp.), 100 g; crude extract, 40 to 200 Aliters; and '4C-uridine-5'-triphosphate (14C-UTP, Amersham/ Searle, specific activity 51 mCi/mmoles) 0.5 ,Ci. The reaction mixture was incubated at 30 C, and at various times 1O0-,4liter samples were removed, precipitated with 2 ml of cold 10% trichloroacetic acid, collected on filters, washed, dried, and counted.…”

Section: Methodsmentioning

confidence: 99%

Development of Bacteriophage Alpha II. Dependence on the Host Ribonucleic Acid Polymerase

Strniste

Taylor

1972

J Virol

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Measurements of ribonucleic acid (RNA) synthesis using '4C-uracil uptake in rifampin-sensitive and rifampin-resistant strains of Bacillus megatherium were carried out after infection of the bacteria with bacteriophage a. Phage development was inhibited in the former but not the latter strain, showing that a phage development is dependent on the bacterial host RNA polymerase. This dependence exists at all times during eclipse. RNA polymerase extracts showed the same in vitro rifampin sensitivity as the corresponding bacterial strains.

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