Physico-chemical and pro-wound healing properties of microporous cellulosic sponge from Gleditsia triacanthos pods functionalized with Phytolacca americana fruit extract

Abstract

This study aimed to exploit two invasive plant species to develop a novel, multifunctional, bioactive wound dressing based on a microporous cellulosic sponge (CS) from Gleditsia triacanthos pods and functionalizing them with Phytolacca americana fruit extract. The CS was functionalized, lyophilized, and characterized by Attenuated total reflectance–Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, contact angle, water absorption, and retention capacity. In addition, two parameters were evaluated in temporal dynamics: controlled release of phenolic compounds and antioxidant activities. The hemolytic index, blood clotting kinetics, lactate dehydrogenase release, and wound scratch assays proved their hemo- and biocompatibility, as well as their ability to promote cell proliferation and migration promoting-activity and to inhibit microbial growth. Furthermore, the obtained spongious material exhibited an anti-inflammatory effect by modulating the macrophages’ secretion profile of IL-6 and IL-10. In conclusion, the microporous cellulosic sponge obtained from G. triacanthos could be used as a vehicle to ensure the controlled release of bioactive principles with pro-wound healing activities extracted from invasive plants. Graphical abstract:

Characterization of Cellulose Derived from Invasive Alien Species Plant Waste for Application in the Papermaking Industry: Physic-Mechanical, Optical, and Chemical Property Analysis

“…Previous studies and research [17,18] have demonstrated that a high content of anthocyanins and total phenols can be found in compounds extracted from Arundo donax, Tradescantia fluminensis, and Eucalyptus globulus. Additionally, other studies [40,41] have shown that Phytolacca americana, beyond the toxicity of its fruits, exhibits anticancer, antioxidant, and antimicrobial properties. The papers produced from this raw material were analyzed using the JEOL 6100 electron microscope located at the CACTI (Scientific and Technical Research Assistance Center) of the University of Vigo, on the As Lagoas campus in Vigo.…”