Physico-chemical characteristics of gamma-irradiated gelatin

Abstract

This article reports the effects of gamma irradiation (dose ranges 0.1–10 kGy from 60Co source) on the characteristics of solid gelatin and the physico-mechanical, microstructural and bioactive properties of the scaffold prepared from irradiated gelatin solution. FTIR, intrinsic viscosity, bloom strength, thermal properties, SEM, tensile properties, water uptake ability and antimicrobial activities of non-irradiated and irradiated solid gelatin and its scaffolds were investigated. The detailed experimental results for the solid gelatin demonstrated that 1 kGy γ-irradiated samples showed higher intrinsic viscosity, enhanced thermal stability and bloom strength than other irradiated samples. Furthermore, the scaffold thus prepared from irradiated and non-irradiated gelatin also revealed that 1 kGy samples showed the highest tensile strength and modulus with good water resistivity than other irradiated and non-irradiated samples. In addition to the physico-mechanical properties, 1 kGy scaffolds have also exhibited the highest resistivity towards microbial growth that can have potentiality as scaffold in biomedical sector. The enhanced functional and bioactive properties at low irradiation doses (1 kGy) may occurred due to an initial breaking of hydrogen bonds of polypeptide chains in gelatin molecules that indicated by the shift of amide A, I and II peaks to higher wave numbers in FTIR. This enhancement resulted probably due to the domination of crosslinking over degradation at 1 kGy. It was also observed that 1 kGy γ-radiation-induced crosslinking has lowered the hydrophilicity by decreasing water uptake and mean pore diameter of the interconnected porous structures of gelatin.

Adaptation of Hard Gelatin Capsules for Aqueous Solution Delivery Using Gamma Radiation

“…The water uptake capacity of both nonirradiated and γ-irradiated HGCs (appeared as HGC shells deformation and swelling) could explain the results obtained for the onset of MB solution leakage, which agree with previous findings 18 , showing lower water uptake for γ-irradiated HGCs than for nonirradiated HGCs up to 2.5 kGy. The authors suggested that radiation doses up to 2.5 kGy induced crosslinking along with interconnection among the gelatin pores due to the formation of a three-dimensional network as a function polypeptide chain crosslinking.…”

“…The authors showed that all hydrogels collapsed as the NaCl concentration increased. The slower in-vitro rupture time of F2 and F3 γ-irradiated HGCs thanF1 non-irradiated HGCs in deionized water (Figure 3) is attributable to the polypeptide chain crosslinking induced by the γ-radiation, which inhibited the water from entering the HGCs 18 . However, at radiation doses >3 kGy, faster invitro rupture times (disintegration/dissolution) were observed as a result of the degradation effect of γ-radiation doses >3 kGy.…”

“…The shift of the amide A peak absorption band to higher wave numbers at radiation doses of 1 and 3 kGy (Figure 1, Table 1) may be attributed to crosslinking and the formation of hydrogen bonds between the amino acid residues of the chains, wherein crosslinking dominates over denaturation 5 . On the other hand, the shift of the amide A peak absorption band to lower wave numbers at higher radiation doses (5 and 10 kGy) could be due to protein molecules degradation dominating over crosslinking at elevated radiation doses 18 . The characteristic amide B, amide I and amide II peaks remained unaffected by the γ-radiation at all used doses.…”

“…On the other hand, the obtained results suggest a decrease in HGC capacity to be filled with aqueous solution without leakage with the use of radiation doses Table 2). The amide bonding of polypeptide chains within the gelatin molecules determines HGC shell pore size 18 . Thus, these results could be confirmed by the FTIR data.…”

Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes

“…The fish gelatine composite membranes with different amounts of bLF were evaluated with respect to antibacterial efficiency against E. coli and S. aureus, for 120 minutes. As shown in figure 5, the electrospun fish gelatine mats by itself displayed a considerable bacterial reduction when compared with the inoculum of 2-log, which is probably attributed to the nature of gelatin and was previously observed against E. coli [56]. This may be due to the swelling nature of the electrospun mats that do not provide the adequate conditions for bacteria cellular growth.…”