Physico-chemical properties and metabolism of lissamine-rhodamine ceramides: effect of acyl chain length

Monti, Eugenio; Demasi, L.; Piccinotti, Alberto; Bresciani, Roberto; Biancardi, L.; Preti, Augusto; Marchesini, Sergio

doi:10.1016/s0009-3084(98)00011-5

Cited by 2 publications

(3 citation statements)

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“…The highest fold increase obtained for fluorescent SM probably reflects the fact that the C12‐LRh‐SM does not accumulate into the cell because the polarity of the fluorochrome determines its release into the medium [22], and PC12 cells are continuously stimulated to synthesize SM. The use of the fluorescent tool has revealed to be more practical and has been preferred to the classical method for SM determination.…”

Section: Discussionmentioning

confidence: 99%

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Nerve growth factor induces sphingomyelin accumulation in pheochromocytoma cells

et al. 2000

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The pheochromocytoma cells are a well-known model for studying the nerve growth factor (NGF)-induced molecular changes during the differentiation process. The involvement of sphingomyelin (SM) was studied using the fluorescent analogue of ceramide, i.e. N-lissamine rhodaminyl-(12-aminododecanoyl) D-erythro-sphingosine (C12-LRh-Cer). This fluorescent analogue is metabolically active and can be used to follow the biosynthesis of SM in intact cells. NGF induces a 4-fold increase of fluorescent SM content in PC12 cells, when loaded with C12-LRh-Cer. Treatment of PC12 cells with actinomycin D or cycloheximide completely abolishes the NGF-induced elevation of SM. Inhibition of p140 trkA receptor by AG-879 prevents extracellular signal-regulated kinase 1/2 phosphorylation and suppresses the increase of SM. Inhibition of protein kinase C (PKC), protein kinase A (PKA) and phosphatidylinositol 3-kinase does not have any effect on NGF-induced C12-LRh-SM accumulation. On the other hand, activation of PKA or PKC with simultaneous treatment with NGF has a synergistic effect on increase of SM content. The NGF-induced SM increase in PC12 cells is an effect promoted by other differentiating agents like dibutyryl cyclic AMP or fibroblast growth factor-2 but not by a mitogenic agent like epidermal growth factor. z 2000 Federation of European Biochemical Societies.

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Section: Discussionmentioning

confidence: 99%

“…C12‐LRh‐Cer and C12‐LRh‐SM (5 μM) complexed with essentially fatty acid‐free human albumin [22] were administered to PC12 cells plated in 60 mm tissue dishes, in a final volume of 2 ml RPMI 1640 medium supplemented with 1% HS and 0.5% FBS, L ‐glutamine and antibiotics.…”

Section: Methodsmentioning

confidence: 99%

Nerve growth factor induces sphingomyelin accumulation in pheochromocytoma cells

et al. 2000

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“…Rhodamines are particularly useful as laser dyes and fluorescent biolables. Monti et al [23] present a comparative study on physico-chemical and metabolism of three different lissamine-rhodamine derivatives of ceramide. MV is an ideal substrate for photometric monitoring.…”

Section: Introductionmentioning

confidence: 99%

Study on the spectral behavior of four fluorescent dyes and their interactions with nucleic acid by the luminescence method

Yuxia¹,

Wei²,

Dong³

2007

Journal of Luminescence

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Physico-chemical properties and metabolism of lissamine-rhodamine ceramides: effect of acyl chain length

Cited by 2 publications

References 20 publications

Nerve growth factor induces sphingomyelin accumulation in pheochromocytoma cells

Nerve growth factor induces sphingomyelin accumulation in pheochromocytoma cells

Study on the spectral behavior of four fluorescent dyes and their interactions with nucleic acid by the luminescence method

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