Physico-chemical properties of murine hepatitis virus, strain A59

Chartier, Daniel; Talbot, Pierre J.

doi:10.1007/bf01320963

Cited by 31 publications

(44 citation statements)

References 30 publications

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“…They are more stable at slightly acidic pH (6 – 6.5) than at alkaline pH (8). This has been shown for the HCoV 229E [112], the MHV [113,114], the TGEV [115] and the canine coronavirus [116]. …”

Section: Hcovs: Enveloped But Not That Fragilementioning

confidence: 80%

Human Coronaviruses: Insights into Environmental Resistance and Its Influence on the Development of New Antiseptic Strategies

Geller

Varbanov

Duval

2012

Viruses

390

374

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The Coronaviridae family, an enveloped RNA virus family, and, more particularly, human coronaviruses (HCoV), were historically known to be responsible for a large portion of common colds and other upper respiratory tract infections. HCoV are now known to be involved in more serious respiratory diseases, i.e. bronchitis, bronchiolitis or pneumonia, especially in young children and neonates, elderly people and immunosuppressed patients. They have also been involved in nosocomial viral infections. In 2002–2003, the outbreak of severe acute respiratory syndrome (SARS), due to a newly discovered coronavirus, the SARS-associated coronavirus (SARS-CoV); led to a new awareness of the medical importance of the Coronaviridae family. This pathogen, responsible for an emerging disease in humans, with high risk of fatal outcome; underline the pressing need for new approaches to the management of the infection, and primarily to its prevention. Another interesting feature of coronaviruses is their potential environmental resistance, despite the accepted fragility of enveloped viruses. Indeed, several studies have described the ability of HCoVs (i.e. HCoV 229E, HCoV OC43 (also known as betacoronavirus 1), NL63, HKU1 or SARS-CoV) to survive in different environmental conditions (e.g. temperature and humidity), on different supports found in hospital settings such as aluminum, sterile sponges or latex surgical gloves or in biological fluids. Finally, taking into account the persisting lack of specific antiviral treatments (there is, in fact, no specific treatment available to fight coronaviruses infections), the Coronaviridae specificities (i.e. pathogenicity, potential environmental resistance) make them a challenging model for the development of efficient means of prevention, as an adapted antisepsis-disinfection, to prevent the environmental spread of such infective agents. This review will summarize current knowledge on the capacity of human coronaviruses to survive in the environment and the efficacy of well-known antiseptic-disinfectants against them, with particular focus on the development of new methodologies to evaluate the activity of new antiseptic-disinfectants on viruses.

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Section: Hcovs: Enveloped But Not That Fragilementioning

confidence: 80%

Human Coronaviruses: Insights into Environmental Resistance and Its Influence on the Development of New Antiseptic Strategies

Geller

Varbanov

Duval

2012

Viruses

390

374

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“…The origin and culture of MHV strains 3, A59, and S and of DBT cells were described previously [14]. For use as antigen for hemag glutination assays, MHV-3 was grown in the absence of fetal calf serum (FCS) and har vested from clarified medium by precipita tion with 10% (w/v) polyethylene glycol in 0.5 M NaCl.…”

mentioning

confidence: 99%

Hemagglutination by Murine Hepatitis Viruses

Talbot¹

1989

Intervirology

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Erythrocytes from twelve mammalian and avian sources in ten different buffers at three incubation temperatures could not be hemagglutinated with murine hepatitis virus (MHV) strains 3, A59, or S grown on DBT cells. Viral antigen preparation in the absence of fetal calf serum, partial virus purification, or various concentrations of red blood cells still failed to yield detectable hemagglutinating activity. Thus, the newly described MHV-DVIM remains the only hemagglutinating strain of murine coronavirus.

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“…The A59 strain of MHV was initially obtained from the American Type Culture Collection (ATCC, Rock-ville, MD, USA), plaque-purified twice, and passaged four times at a multiplicity of infection (MOI) of 0.01 on DBT cells as described previously (Daniel and Talbot, 1987). DBT astrocytoma cells induced as described previously (Kumanishi, 1967) were a kind gift of Dr. Michael J. Buchmeier (The Scripps Research Institute, La Jolla, CA, USA).…”

Section: Virus Cells and Animalsmentioning

confidence: 99%

“…Mixtures were centrifuged at 4 8C for 20 min at 514Âg. Viral load from supernatants was subsequently evaluated by plaque assays on DBT cells as previously described (Daniel and Talbot, 1987).…”

Section: Viral Loadmentioning

confidence: 99%

Transcriptome profile within the mouse central nervous system and activation of myelin-reactive T cells following murine coronavirus infection

Gruslin

Moisan

St‐Pierre

et al. 2005

Journal of Neuroimmunology

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Multiple sclerosis (MS) is an autoimmune disease associated with environmental factors, possibly including several viruses such as the coronaviruses. Indeed, murine coronavirus (MHV) infection provides a well-known experimental model for MS studies. Intracerebral infection of C57BL/6 mice with MHV-A59 revealed that viral replication was efficient and that clearance of infectious virus occurred as soon as 7 days post-infection. Using cDNA arrays, analysis of gene expression profile in the brain revealed a modulation of 80 different genes following infection, with at least 27 of these genes having previously been directly related to innate or acquired immune responses. Concordingly, an important activation of auto-reactive T cells specific to myelin basic protein was demonstrated. Altogether, these results indicate that an MHV infection of the central nervous system (CNS) leads to an important host genomic response implicating immunity-related genes and to the activation of myelin-reactive autoimmune T cells.

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Physico-chemical properties of murine hepatitis virus, strain A59

Cited by 31 publications

References 30 publications

Human Coronaviruses: Insights into Environmental Resistance and Its Influence on the Development of New Antiseptic Strategies

Human Coronaviruses: Insights into Environmental Resistance and Its Influence on the Development of New Antiseptic Strategies

Hemagglutination by Murine Hepatitis Viruses

Transcriptome profile within the mouse central nervous system and activation of myelin-reactive T cells following murine coronavirus infection

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