Physico-chemical properties of PDMS surfaces suitable as substrates for cell cultures

Raczkowska, Joanna; Prauzner-Bechcicki, Szymon; Lukeš, Jaroslav; Šepitka, Josef; Bernasik, Andrzej; Awsiuk, Kamil; Paluszkiewicz, C.; Pabijan, Joanna; Lekka, Małgorzata; Budkowski, Andrzej

doi:10.1016/j.apsusc.2016.07.009

Cited by 38 publications

(33 citation statements)

References 66 publications

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“…3), the absorption peaks at 2971 and 2904 cm −1 are attributed to asymmetric and symmetric stretching of C─H bonds of methyl group (CH 3 ), respectively. 39 The peak at 2139 cm −1 corresponds to Si─H bond stretching. 40 The peak at 1409 cm −1 is related to the asymmetric deformation of CH 3 .…”

Section: Resultsmentioning

confidence: 99%

Highly selective carbon nanotube‐coupled graphene oxide‐incorporated polydimethylsiloxane membrane for pervaporative membrane bioreactor ethanol production

Amrei

Asghari

Esfahanian

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND In this work, a pervaporation membrane bioreactor (PVMBR) with the novel ethanol permselective membrane was used to achieve ethanol production with high yield. For this purpose, ethanol produced by Saccharomyces cerevisiae from glucose and pervaporation was performed via dense hydrophobic polydimethylsiloxane (PDMS) having carbon nanotube‐graphene oxide (CNT‐GO) mixed matrix membrane. Different percentages of CNT‐GO (0, 0.25, 0.5, 0.75, and 1) were loaded in polymer matrix. Synthesized membranes were analyzed using FESEM, AFM, FTIR and XRD. RESULTS The bonding of groups in CNT‐GO, that was synthesized in this work, and membranes was characterized and confirmed using FTIR test. Considering the existence of 0.75 wt% of CNT‐GO in PDMS showed the highest ethanol selectivity of 22.5 in synthetic ethanol‐water solution, this incorporation was selected to use in PVMBR. The high ethanol selectivity was proved using XRD patterns. Also, FESEM indicated the optimum dispersion of nanoparticles at 0.75% of loading and AFM proofed the optimum surface roughness at this percentage. CONCLUSIONS The results showed that maximum ethanol production in PVMBR was achieved after 44 h with productivity of 0.5g.l−1.h−1 in feed side and 9.89 g.l−1.h−1 in permeate side. In fact, PVMBR enhanced the ethanol concentration up to 26 times. © 2020 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Highly selective carbon nanotube‐coupled graphene oxide‐incorporated polydimethylsiloxane membrane for pervaporative membrane bioreactor ethanol production

Amrei

Asghari

Esfahanian

et al. 2020

J of Chemical Tech & Biotech

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“…Therefore we have performed systematic studies on film thickness dependent elasticity of soft PDMS substrate (E = 1.7 MPa [63]) decorated with stiffer polymer layers composed of PS (E = 3.4 GPa [44,49]) or PEO (E = 0.2–0.6 GPa [74,75]). The polymer substrates were characterized by the presence of strong attractive forces observed during the load.…”

Section: Resultsmentioning

confidence: 99%

“…For this purpose, we performed systematical studies on the impact of the thickness of decorating layer on effective elasticity of soft substrate (Young modulus E = 1.67 MPa [63]) covered with thin polymer film.…”

Section: Introductionmentioning

confidence: 99%

Elasticity patterns induced by phase-separation in polymer blend films

et al. 2017

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Systematical studies on the impact of the thickness of thin films composed of polystyrene (PS) or poly(ethylene oxide) (PEO) on the effective elasticity of polymer-decorated soft polydimethylsiloxane substrate were performed. For both investigated polymer films, elasticity parameter was determined from force-displacement curves recorded using atomic force microscopy. Effective stiffness of supported film grows monotonically with film thickness, starting from the value comparable to the elasticity of soft support and reaching plateau for polymer layers thicker than 200 nm. In contrary, for films cast on hard support no significant thickness dependence of elasticity was observed and the value of elasticity parameter was similar to the one of the substrate. Based on these results, non-conventional method to produce elasticity patterns of various shapes and dimensions induced by phase-separation process in symmetric and asymmetric PS:PEO blend films on soft support was demonstrated. Elevated PS domains were characterized by elasticity parameter 2 times higher than lower PEO matrix. In contrary, adhesion force was increased more than 3 times for PEO regions, as compared to PS areas.

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“…We have targeted the potential utility of this soft platform to be T‐cell‐based ACT partly because the expanded T cells can be easily detached from the surfaces and infused to patients, leaving the PDMS material behind. Knowing the effect of elastic moduli on improved adhesion and proliferation of various cell types such as fibroblasts, smooth muscle cells and epithelial cells; perhaps, the effect of rigidity demonstrated here might have direct applications in tissue engineering of both immune and non‐immune systems. Future studies will focus on optimizing this approach to maximize Treg production using even softer materials that can reach physiological values, as well as application of this concept to cells of human origin, a key step in emerging immunotherapy approaches to autoimmune‐based diseases.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo induction of regulatory T cells from conventional CD4⁺ T cells is sensitive to substrate rigidity

Nataraj

Dang

Kam

et al. 2018

J Biomedical Materials Res

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The immune system maintains a balance between protection and tolerance. Regulatory T cells (Tregs) function as a vital tolerance mechanism in the immune system to suppress effector immune cells. Additionally, Tregs can be utilized as a form of immunotherapy for autoimmune disorders. As T cells have previously been shown to exhibit sensitivity to the rigidity of an activating substrate upon activation via IL-2 secretion, we herein explore the previously unknown effect of substrate rigidity on the induction of Tregs from conventional naïve mouse CD4+ T cells. Substrates with modulatable rigidities ranging from a hundred kilopascals to a few megapascals were fabricated via poly(dimethylsiloxane). We found that there was a significant increase in Treg induction at lower substrate rigidities (i.e., E ~ 100 kPa) compared to higher rigidity levels (i.e., E ~ 3 MPa). To confirm that this significant difference in induction rate was truly related to T cell mechanosensing, we administered compound Y-27632 to inhibit myosin contractility. In the presence of Y-27632, the myosin-based contractility was disrupted and, as a result, the difference in Treg induction caused by the substrate rigidity was abrogated. This study demonstrates that mechanosensing is involved in Treg induction and raises questions about the underlying molecular mechanisms involved in this process.

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Physico-chemical properties of PDMS surfaces suitable as substrates for cell cultures

Cited by 38 publications

References 66 publications

Highly selective carbon nanotube‐coupled graphene oxide‐incorporated polydimethylsiloxane membrane for pervaporative membrane bioreactor ethanol production

Highly selective carbon nanotube‐coupled graphene oxide‐incorporated polydimethylsiloxane membrane for pervaporative membrane bioreactor ethanol production

Elasticity patterns induced by phase-separation in polymer blend films

Ex vivo induction of regulatory T cells from conventional CD4⁺ T cells is sensitive to substrate rigidity

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Physico-chemical properties of PDMS surfaces suitable as substrates for cell cultures

Cited by 38 publications

References 66 publications

Highly selective carbon nanotube‐coupled graphene oxide‐incorporated polydimethylsiloxane membrane for pervaporative membrane bioreactor ethanol production

Highly selective carbon nanotube‐coupled graphene oxide‐incorporated polydimethylsiloxane membrane for pervaporative membrane bioreactor ethanol production

Elasticity patterns induced by phase-separation in polymer blend films

Ex vivo induction of regulatory T cells from conventional CD4+ T cells is sensitive to substrate rigidity

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Ex vivo induction of regulatory T cells from conventional CD4⁺ T cells is sensitive to substrate rigidity