Physico-chemical properties of physiologically active polysaccharides from wheat tissue culture

Abstract

Polysaccharides (PS) from wheat cell culture were isolated by liquid-liquid extraction. The molecular mass distribution was determined by gel-permeation chromatography (GPC) using dual detectors for the simultaneous detection. It was supposed that PS sample from wheat cell culture has molecular weight of 1632 Da. The physic-chemical properties of PS such as solubility in different solvents, surface activity, ξ-potential, the pH value, polydispersity (PDI) were determined. The PS sample was soluble in water and insoluble in ethanol, acetone and chloroform. ξ-potential of PS was evaluated in order to determine its charge at different pH value from 3 to 9. As a result, the ξ values for the PS solution were negative throughout the pH range studied, varying from -2.85 mV (pH 3.0) to -21.1 (pH 9). Using tensiometry method, surface tension of the PS at the liquid/air interface was investigated. At 0.05% concentration interfacial tension decreases slowly and reaches an equilibrium value after ~ 8-8.5 hours. The pH was equal to 5.6±0.05. For a PS solution of 0.001% at pH 5.5 PDI was equal to 0.595.

Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture

“…Our previous research enabled us to identify bioactive polysaccharides in cereal cell suspension cultures [ 30 ]. We explored the chemical composition and physical properties of these wheat PSs, which were found to be represented by arabinogalactans and supposed to be represented by xyloglucans, arabinoxylans, and glucans, according to their monosaccharide composition [ 31 , 32 ]. In addition, we showed that monosaccharide composition changed significantly depending on the cultivation media [ 33 ].…”