Physico-mechanical and biological evaluation of heparin/VEGF-loaded electrospun polycaprolactone/decellularized rat aorta extracellular matrix for small-diameter vascular grafts

Cuenca, John Patrick; Kang, Hoe-Jin; Fahad, Md. Abdullah Al; Park, Myeongki; Choi, Minsu; Lee, Hyun-Yong; Lee, Byong‐Taek

doi:10.1080/09205063.2022.2069398

Cited by 17 publications

(7 citation statements)

References 54 publications

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“…Isoflurane (Piramal, India) was used for anesthetized the rats throughout the surgery. We followed standard surgical procedure which is vastly accepted in this research field 35,36 . Concisely, a midline incision on the abdominal part was made and using a warm wet gauze, rat intestines were wrapped and carefully pushed aside.…”

Section: Methodsmentioning

confidence: 99%

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Physicochemical, in vitro and in vivo evaluation of VEGF loaded PCL‐mPEG and PDGF loaded PCL‐Chitosan dual layered vascular grafts

Sultana,

Fahad,

Park

et al. 2023

J Biomed Mater Res

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The present study has attempted to evaluate the endothelialization and smooth muscle regeneration efficiency of a novel dual‐layer small‐diameter vascular graft. Two types of layers (PCL‐mPEG‐VEGF and PCL‐Chitosan‐PDGF) were fabricated to find out the best layer giving endothelialization support for the lumen and unique contractile function for outer layer of blood vessels. Platelet‐derived growth factor (PDGF) and chitosan were immobilized onto PCL surface by aminolysis‐based surface modification technique. Besides, Poly (ethylene glycol) methyl ether (mPEG) and vascular endothelial growth factor (VEGF) were directly blended with PCL. Morphological analysis of membranes ensured consistency of average fibers diameter with native extracellular matrix. A favorable interaction of PCL‐mPEG‐VEGF with cow pulmonary endothelial cells (CPAEs) and PCL‐Chitosan‐PDGF with rat bone marrow mesenchymal stem cells (RBMSCs) was obtained during in vitro study. Controlled growth factor release patterns were found from both layers. Further, PCL‐mPEG‐VEGF exhibited endothelial markers expression properties from RBMSCs. Up‐regulation of SMCs markers expression was significantly ensured by the PCL‐Chitosan‐PDGF membrane. Thus, PCL‐mPEG‐VEGF and PCL‐Chitosan‐PDGF were preferred as inner and outer layers respectively of a finally prepared tubular hybrid tissue engineered small diameter vascular graft. Finally, the dual‐layer vascular graft was implanted onto a rat abdominal aorta model for 2 months. The extracted samples exhibited the presence of endothelial marker (ICAM 1) in the inner layer and smooth muscle cell marker (αSMA) in the outer layer as well as substantial amount of collagen deposition was observed in the both layers.

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Section: Methodsmentioning

confidence: 99%

“…We followed standard surgical procedure which is vastly accepted in this research field. 35,36 Concisely, a midline incision on the abdominal part was made and using a warm wet gauze, rat intestines were wrapped and carefully pushed aside. Then, aorta was exposed carefully under a microscope.…”

Section: In Vivo Implantationmentioning

confidence: 99%

Physicochemical, in vitro and in vivo evaluation of VEGF loaded PCL‐mPEG and PDGF loaded PCL‐Chitosan dual layered vascular grafts

Sultana,

Fahad,

Park

et al. 2023

J Biomed Mater Res

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“…VEGF, an essential growth factor regulating neovascularization and angiogenesis, has also been processed into a dECM-based scaffold for tissue regeneration [ 168 ]. Liu et al .…”

Section: Decm–bioactive Factor Composite Materialsmentioning

confidence: 99%

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Xu,

Kankala,

Wang

et al. 2023

Regenerative Biomaterials

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Despite the considerable advancements in fabricating polymeric-based scaffolds for tissue engineering, the clinical transformation of these scaffolds remained a big challenge because of the difficulty of simulating native organs/tissues' microenvironment. As a kind of natural tissue-derived biomaterials, decellularized extracellular matrix (dECM)-based scaffolds have gained attention due to their unique biomimetic properties, providing a specific microenvironment suitable for promoting cell proliferation, migration, attachment, and regulating differentiation. The medical applications of dECM-based scaffolds have addressed critical challenges, including poor mechanical strength and insufficient stability. For promoting the reconstruction of damaged tissues or organs, different types of dECM-based composite platforms have been designed to mimic tissue microenvironment, including by integrating with natural polymer or/and syntenic polymer or adding bioactive factors. In this review, we summarized the research progress of dECM-based composite scaffolds in regenerative medicine, highlighting the critical challenges and future perspectives related to the medical application of these composite materials.

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“…A Polycaprolactone (PCL) scaffold was developed with sequential layers of heparin and VEGF was developed. Long-term anti-thrombogenic effect of tissue engineered graft was achieved by initial burst release of VEGF, facilitated by ECM degrading enzyme metallopeptidase-2 (MMP-2) and controlled release of heparin [ 36 ].…”

Section: Growth Factors Regulation In Angiogenesismentioning

confidence: 99%

Engineering Spatiotemporal Control in Vascularized Tissues

2022

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A major challenge in engineering scalable three-dimensional tissues is the generation of a functional and developed microvascular network for adequate perfusion of oxygen and growth factors. Current biological approaches to creating vascularized tissues include the use of vascular cells, soluble factors, and instructive biomaterials. Angiogenesis and the subsequent generation of a functional vascular bed within engineered tissues has gained attention and is actively being studied through combinations of physical and chemical signals, specifically through the presentation of topographical growth factor signals. The spatiotemporal control of angiogenic signals can generate vascular networks in large and dense engineered tissues. This review highlights the developments and studies in the spatiotemporal control of these biological approaches through the coordinated orchestration of angiogenic factors, differentiation of vascular cells, and microfabrication of complex vascular networks. Fabrication strategies to achieve spatiotemporal control of vascularization involves the incorporation or encapsulation of growth factors, topographical engineering approaches, and 3D bioprinting techniques. In this article, we highlight the vascularization of engineered tissues, with a focus on vascularized cardiac patches that are clinically scalable for myocardial repair. Finally, we discuss the present challenges for successful clinical translation of engineered tissues and biomaterials.

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Physico-mechanical and biological evaluation of heparin/VEGF-loaded electrospun polycaprolactone/decellularized rat aorta extracellular matrix for small-diameter vascular grafts

Cited by 17 publications

References 54 publications

Physicochemical, in vitro and in vivo evaluation of VEGF loaded PCL‐mPEG and PDGF loaded PCL‐Chitosan dual layered vascular grafts

Physicochemical, in vitro and in vivo evaluation of VEGF loaded PCL‐mPEG and PDGF loaded PCL‐Chitosan dual layered vascular grafts

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Engineering Spatiotemporal Control in Vascularized Tissues

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