Physicochemical and biochemical characterization of transgenic papaya modified for protection against <i>Papaya ringspot virus</i>

Roberts, Madeen; Minott, Donna A.; Pinnock, Simone; Tennant, Paula; Jackson, José

doi:10.1002/jsfa.6374

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…All fatty acids were expressed as percentage with respect to the total fatty acids (Table ). Among them, consistent with previous results, − palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:3) were the most abundant fatty acids of transgenic virus-resistant soybeans and their nontransgenic counterpart. The percentage values of the majority of the measured fatty acids fell within the ranges of the ILSI database, except palmitoleic and heptadecenoic; the contents of these two fatty acids were lower than the minimum values.…”

Section: Results and Discussionsupporting

confidence: 92%

“…The present study was designed to evaluate the composition of the RNAi-mediated virus-resistant transgenic soybeans engineered with hairpin construct and their nontransgenic counterpart through a series of chemical analyses to determine whether significant undesirable biologically changes occurred by the IR insertion in transgenic soybeans, using the concept of “substantial equivalence”, which was developed by the Organisation for Economic Co-operation and Development (OECD) in 1993 and was elaborated further by FAO/WHO. , The concept suggests that if no meaningful difference from the conventional counterpart is found, then the transgenic crop is as safe and nutritious as its traditional counterpart, which is generally accepted as safe on the basis of human food use history. Substantial equivalent analysis has been studied for different events of transgenic crops, such as corn, , soybean, − rice, and papaya …”

Section: Introductionmentioning

confidence: 99%

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Compositional Equivalency of RNAi-Mediated Virus-Resistant Transgenic Soybean and Its Nontransgenic Counterpart

Zhang

Zhao

et al. 2014

J. Agric. Food Chem.

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RNA silencing or RNA interference (RNAi), which is triggered by double-stranded RNA (dsRNA), is an evolutionarily conserved process that is active in a wide variety of eukaryotic organisms. Engineering plants with hairpin construct in which the viral gene is arranged in inverted repeats (IR) renders plants resistant to plant virus infection. However, there is no report on whether biologically important changes occurred by the insertion of IR, which confer transgenic plants virus resistance. In the present study, the compositions of virus-resistant transgenic soybean seeds developed by insertion of three short IRs, each containing the specific, highly conserved sequences derived from one virus, were compared with those of nontransgenic counterparts by applying the principle of substantial equivalence to determine whether significant undesirable biological changes occurred by IR insertion. The results revealed that the nutrient components as well as antinutrient contents of these virus-resistant soybean lines are substantially equivalent to those of the nontransgenic counterparts, and the majority of the measured amounts of nutritional components and antinutrient contents are well within the range of values reported for other commercial soybean lines. The results imply that no biologically important changes occurred by the insertion of IRs in the RNAi-mediated virus-resistant transgenic soybeans. The results can serve as baseline information for developing RNAi-mediated transgenic soybean cultivars or other crops with broader spectrum virus resistance.

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Section: Results and Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Compositional Equivalency of RNAi-Mediated Virus-Resistant Transgenic Soybean and Its Nontransgenic Counterpart

Zhang

Zhao

et al. 2014

J. Agric. Food Chem.

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“…In the analyzed studies, associations between ethnicity and socioeconomic variables showed no agreement. 15 - 17 , 19 , 22 , 25 One of the explanations, according to Roberts et al, 17 would be that sleep problems are associated with the social status of a minority, not being associated with ethnicity. Another important point, according to some authors, 16 , 17 is the fact that young individuals who belong to minority groups can express mental suffering in a negative way, especially among immigrants.…”

Section: Discussionmentioning

confidence: 99%

“…Nos estudos analisados, as relações entre etnias e as variáveis socioeconômicas não entraram em consonância. 15 - 17 , 19 , 22 , 25 Uma das explicações, segundo Roberts et al., 17 seria que os problemas do sono estariam relacionados à condição social de minoria, e não à origem étnica. Outro ponto a ser destacado, segundo alguns autores, 16 , 17 é o fato de jovens pertencentes ao grupo das minorias poderem expressar de forma negativa o sofrimento psíquico, especialmente entre os imigrantes.…”

Section: Discussionunclassified

Sono em adolescentes de diferentes níveis socioeconômicos: revisão sistemática

Felden

Leite

Rebelatto

et al. 2015

Revista Paulista de Pediatria

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Objective:To analyze the sleep characteristics in adolescents from different socioeconomic levels.Data source:Original studies found in the MEDLINE/PubMed and SciELO databases without language and period restrictions that analyzed associations between sleep variables and socioeconomic indicators. The initial search resulted in 99 articles. After reading the titles and abstracts and following inclusion and exclusion criteria, 12 articles with outcomes that included associations between sleep variables (disorders, duration, quality) and socioeconomic status (ethnicity, family income, and social status) were analyzed.Data synthesis:The studies associating sleep with socioeconomic variables are recent, published mainly after the year 2000. Half of the selected studies were performed with young Americans, and only one with Brazilian adolescents. Regarding ethnic differences, the studies do not have uniform conclusions. The main associations found were between sleep variables and family income or parental educational level, showing a trend among poor, low social status adolescents to manifest low duration, poor quality of sleeping patterns.Conclusions:The study found an association between socioeconomic indicators and quality of sleep in adolescents. Low socioeconomic status reflects a worse subjective perception of sleep quality, shorter duration, and greater daytime sleepiness. Considering the influence of sleep on physical and cognitive development and on the learning capacity of young individuals, the literature on the subject is scarce. There is a need for further research on sleep in different realities of the Brazilian population.

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Complete genome of Hainan papaya ringspot virus using small RNA deep sequencing

Zhang

Huang

et al. 2014

Virus Genes

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Small RNA deep sequencing allows for virus identification, virus genome assembly, and strain differentiation. In this study, papaya plants with virus-like symptoms collected in Hainan province were used for deep sequencing and small RNA library construction. After in silicon subtraction of the papaya sRNAs, small RNA reads were used to in the viral genome assembly using a reference-guided, iterative assembly approach. A nearly complete genome was assembled for a Hainan isolate of papaya ringspot virus (PRSV-HN-2). The complete PRSV-HN-2 genome (accession no.: KF734962) was obtained after a 15-nucleotide gap was filled by direct sequencing of the amplified genomic region. Direct sequencing of several random genomic regions of the PRSV isolate did not find any sequence discrepancy with the sRNA-assembled genome. The newly sequenced PRSV-HN-2 genome shared a nucleotide identity of 96 and 94 % to that of the PRSV-HN (EF183499) and PRSV-HN-1 (HQ424465) isolates, and together with these two isolates formed a new PRSV clade. These data demonstrate that the small RNA deep sequencing technology provides a viable and rapid mean to assemble complete viral genomes in plants.

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Physicochemical and biochemical characterization of transgenic papaya modified for protection against Papaya ringspot virus

Abstract: The findings suggest that transformation did not produce any major unintended alterations in the physicochemical attributes of the transgenic papayas. Transgene proteins in the edible fruit pulp were low or undetectable.

Cited by 6 publications

References 17 publications

Compositional Equivalency of RNAi-Mediated Virus-Resistant Transgenic Soybean and Its Nontransgenic Counterpart

Compositional Equivalency of RNAi-Mediated Virus-Resistant Transgenic Soybean and Its Nontransgenic Counterpart

Sono em adolescentes de diferentes níveis socioeconômicos: revisão sistemática

Complete genome of Hainan papaya ringspot virus using small RNA deep sequencing

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