PHYSICOCHEMICAL AND IMMUNOCHEMICAL CHARACTERIZATION OF THE ALLERGENS FROM THE MITE <i>DERMATOPHAGOIDES PTERONYSSINUS</i>

Stewart, G.A.; Turner, Kati

doi:10.1038/icb.1980.26

Cited by 38 publications

(26 citation statements)

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“…The Group I allergens (Derp I, Derf I, Der m I) are heat-labile glycoproteins (relative molecular mass, Mr, 25 000), which are heterogeneous on isoelectricfocusing (IEF) (pI, 4.7-7.4) and are excreted in faeces (5,9,10,27,(31)(32)(33). These allergens appear to be structural homologues and have very similar Nterminal amino acid sequences.…”

Section: Identification and Standardizationmentioning

confidence: 99%

Dust mite allergens and asthma—A worldwide problem

Platts‐Mills¹,

deWeck²,

Aalberse³

et al. 1989

Journal of Allergy and Clinical Immunology

599

304

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After the discovery ofhouse dust mites in 1964 their association with asthma has been reported from many different parts of the world including the developing countries. Two sets of major allergens from mites of the genus Dermatophagoides are now well recognized. The Group I allergens are glycoproteins of relative molecular mass (Mr) 25 000, which show both structural homology and cross-reactivity. The allergen Der p I has been cloned and sequenced confirming the M, and establishing its nature as a protease. The Group II allergens (Mr 15 000) show even closer homology and cross-reactivity.Specific immunoassays for Group I and Group II allergens, using monospecific antisera and monoclonal antibodies, have been standardized and are suitable for measuring allergen levels in different parts of the world.Measures for reducing the levels of mite allergens in houses include the covering of mattresses, hot washing of bedding, and removal of carpets from bedrooms as well as humidity control, vacuum cleaning, and the use ofacaricides in the rest ofthe house. There is already evidence that these procedures can cause a major improvement in the symptoms of asthma. While provisional standards for both sensitization to mites and also mite allergen exposure can now be recommended, there is an urgent needfor controlled studies using protocols demonstrated to reduce mite allergen levels by at least tenfold and for further international collaboration.

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Section: Identification and Standardizationmentioning

confidence: 99%

Dust mite allergens and asthma—A worldwide problem

Platts‐Mills¹,

deWeck²,

Aalberse³

et al. 1989

Journal of Allergy and Clinical Immunology

599

304

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“…Ste ward as a lyophilised extract from house dust mite [16]. L. perenne (rye-grass pollen), purchased from Greer Laboratories, USA, was defatted in dry ether, extracted with 0.1 M NH.,HCO,.…”

Section: Methodsmentioning

confidence: 99%

The Characteristics of Antigen Define the Cellular Source and Kinetics of Histamine-Releasing Factor Induced by Human Peripheral-Blood Mononuclear Cells

Turner

Strickland

Siemensma

et al. 1991

Int Arch Allergy Immunol

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Peripheral-blood leucocytes from Dermatophagoides pteronyssinus- and/or Lolium perenne-allergic patients produce in vitro histamine-releasing factor (HRF) in an allergen-specific and concentration-dependent relationship. Maximum production of HRF occurred in cultures containing as little as 10–100 pg/ml crude allergen extract, although a second peak occurred at higher concentrations (10–100 μg/ml). While HRF was detectable in 1-hour cultures, maximal production required 24 h in culture. In contrast, HRF induced by streptokinase/streptodornase (SK/SD) was generally maximal after 1 h. Allergen-induced HRF production was almost exclusively associated with monocytes and B cells. In contrast, peripheral-blood T cells were the major source of induced HRF production in cultures containing SK/SD. Histamine-releasing cytokines are apparently produced by different cell populations, the activation of which may be dependent upon the characteristics of the stimulating antigen.

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“…Studies from this laboratory have shown that ex tracts of the house dust mite, Dermatophagoides pter onyssinus, contain an allergen designated Dpt 4 [19,20] which exhibits certain physicochemical character istics differing markedly from those associated with previously described allergens from other sources. For example, by gradient polyacrylamide gel electro phoresis the bulk of the allergen was found to have an apparent molecular weight of 274,000 daltons, a value outside the commonly accepted molecular weight range of 10,000-60,000 daltons circumscribing the al lergens from grass pollens and danders [13].…”

Section: Introductionmentioning

confidence: 99%

“…For example, by gradient polyacrylamide gel electro phoresis the bulk of the allergen was found to have an apparent molecular weight of 274,000 daltons, a value outside the commonly accepted molecular weight range of 10,000-60,000 daltons circumscribing the al lergens from grass pollens and danders [13]. In addi tion, Dpt 4 demonstrated heterogeneity with regard to both molecular weight and binding to the lectin Con-A [19,20], Further, this allergen was shown to contain the hapten phosphorylcholine (PC) as judged by its reactivity with the PC-specific murine IgA mye loma S107 [20,21].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Very High Density Lipoprotein Allergen, Dpt 4, from the House Dust Mite <i>Dermatophagoides pteronyssinus</i>

Stewart¹,

Butcher²,

Turner³

1983

Int Arch Allergy Immunol

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The allergen Dpt 4 from the house dust mite Dermatophagoides pteronyssinus was shown to be a lipoprotein as judged by its Sudan black B staining properties. Ultracentrifugal studies confirmed this finding and showed that Dpt 4 was polydisperse, although the majority of the allergen belonged to a very high density lipoprotein class. Physicochemical studies using gradient polyacrylamide gel electrophoresis showed that Dpt 4 had an apparent molecular weight of 244,000 daltons and a particle diameter of 10.73 nm. Agarose gel electrophoresis showed that the allergen had an electrophoretic mobility 0.86 times that of human α-lipoprotein. The observation that Dpt 4 is a lipoprotein is consistent with earlier studies which showed that the allergen was heterogeneous with regard to its molecular weight and that it contained the hapten phosphorylcholine.

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PHYSICOCHEMICAL AND IMMUNOCHEMICAL CHARACTERIZATION OF THE ALLERGENS FROM THE MITE DERMATOPHAGOIDES PTERONYSSINUS

Cited by 38 publications

References 0 publications

Dust mite allergens and asthma—A worldwide problem

Dust mite allergens and asthma—A worldwide problem

The Characteristics of Antigen Define the Cellular Source and Kinetics of Histamine-Releasing Factor Induced by Human Peripheral-Blood Mononuclear Cells

Characterization of a Very High Density Lipoprotein Allergen, Dpt 4, from the House Dust Mite <i>Dermatophagoides pteronyssinus</i>

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