Physicochemical and Immunochemical Proofs of Short-Term Insulin Regulation of Hepatic M(2) Pyruvate Kinase

Abstract

In isolated rat hepatocytes, the short-term regulation of the M(2) pyruvate kinase isozyme by insulin is demonstrated. This hormone dependence, which is a function of dose and incubation time, is checked by the activity of the pyruvate kinase, using enzyme assay, cellulose acetate electrophoresis and electroimmunodiffusion methods.