Physicochemical and Structural Characterization of Alkali-Treated Biopolymer Sphingan WL Gum from Marine Sphingomonas sp. WG

Abstract

Sphingan WL gum (WL), a kind of exopolysaccharide, is produced by Sphingomonas sp. WG, which was screened from sea mud samples of Jiaozhou Bay by our group. The solubility of WL was investigated in this work. First, 1 mg/mL of WL solution was stirred at room temperature for at least 2 h to obtain a uniform opaque liquid, and further the solution became clear with the increased NaOH and stirring time. Subsequently, the structural features, solubility, and rheological properties of WL before and after alkali treatment were compared systematically. FTIR, NMR, and zeta potential results indicate that the alkali causes acetyl group hydrolysis and carboxyl group deprotonation. XRD, DLS, GPC, and AFM results suggest that the alkali destroys the ordered arrangement and inter- and intrachain entanglement of polysaccharide chains. In the same case, 0.9 M NaOH-treated WL presents better solubility (stirring for 15 min to obtain a clarified solution) but, unsurprisingly, worsens rheological properties. All results demonstrated that the good solubility and transparency of alkali-treated WL will help promote its postmodification and application.