Physicochemical, antioxidant properties of giant croaker (Nibea japonica) swim bladders collagen and wound healing evaluation

Chen, Yingyue; Jin, Huoxi; Yang, Fei; Jin, Shujie; Liu, Chenjuan; Zhang, Liukai; Huang, Ju; Wang, Shiguang; Yan, Zhongyong; Cai, Xu-Wei; Zhao, Rui; Yu, Fangmiao; Yang, Zuisu; Ding, Guofang

doi:10.1016/j.ijbiomac.2019.07.111

Cited by 74 publications

(76 citation statements)

References 42 publications

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“…The concentrations of ASC and PSC from Nibea japonica swim bladders were higher than that of PSC from Lophius litulon skin when the clearance rate of ABTS• reached 50%. Moreover, the scavenging activities of both HO• and O 2 -• by ASC and PSC from Nibea japonica swim bladders did not reach 50% even at concentrations of 10 mg/mL, which were obviously lower than that of PSC from Lophius litulon skin [7]. These results indicated that the PSC from Lophius litulon skin has a moderate antioxidant capacity.…”

Section: Antioxidant Activitymentioning

confidence: 82%

“…PSC can scavenge the free radicals in a dose-dependent manner and increase the levels of SOD and CAT, suggesting that the PSC from Lophius litulon skin showed antioxidant activity. Furthermore, the antioxidant activity of PSC was higher than that of collagen peptides from tilapia skin and collagens from Nibea japonica swim bladders [7,38]. Excessive reactive oxygen species (ROS) can lead to a variety of chronic health problems, and increased antioxidant defense can reduce ROS production and accelerate wound healing [42].…”

Section: Resultsmentioning

confidence: 99%

“…The scavenging activity of PSC for DPPH•, ABTS•, HO•, and O 2 -radicals was measured according to the procedures described by Chen et al [7], with ascorbic acid used as a positive control.…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…The study of Lukasiewicz et al suggested that the collagen coating extracted by acetic acid greatly reduced the visceral adherence to the polypropylene mesh and did not increase complications or cause changes in the binding of the polypropylene mesh [6]. Chen et al studied the antioxidant activity of ASC and PSC obtained from Nibea japonica swim bladders, and pointed out that PSC has potential application value in wound healing [7].…”

Section: Introductionmentioning

confidence: 99%

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Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

et al. 2019

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The structure of pepsin-solubilized collagen (PSC) obtained from the skin of Lophius litulon was analyzed using the sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). SDS-PAGE results showed that PSC from Lophius litulon skin was collagen type I and had collagen-specific α1, α2, β, and γ chains. FTIR results indicated that the infrared spectrum of PSC ranged from 400 to 4000 cm−1, with five main amide bands. SEM revealed the microstructure of PSC, which consisted of clear fibrous and porous structures. In vitro antioxidant studies demonstrated that PSC revealed the scavenging ability for 2,2-diphenyl-1-picrylhydrazyl (DPPH), HO·, O2−·, and ABTS·. Moreover, animal experiments were conducted to evaluate the biocompatibility of PSC. The collagen sponge group showed a good biocompatibility in the skin wound model and may play a positive role in the progression of the healing process. The cumulative results suggest that collagen from the skin of Lophius litulon has potential applications in wound healing due to its good biocompatibility.

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Section: Antioxidant Activitymentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Antioxidant Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

et al. 2019

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“…where A = mL of NaOH used; B = the concentration of the solution used for titration (0.05 M NaOH); C = volume of sample diluent (mL); D = constant volume of sample (mL). The DPPH, hydroxyl, ABTS, and superoxide anion radical scavenging activities of LPs <1 kDa were performed using the methods described by Chen et al [36].…”

Section: Dh and Antioxidant Activitymentioning

confidence: 99%

Optimization of Extraction of Bioactive Peptides from Monkfish (Lophius litulon) and Characterization of Their Role in H2O2-Induced Lesion

Tian

Zheng

et al. 2020

Marine Drugs

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Background: Marine fish meat has been widely used for the extraction of bioactive peptides. This study was aimed to optimize the preparation of monkfish muscle peptides (LPs) using response surface methodology (RSM) and explore the antioxidant activities of <1 kDa LPs. Methods: Peptides were prepared from the muscles of monkfish (Lophius litulon), and five proteases were tested to hydrolyze muscle proteins. The hydrolysate that was treated using neutrase showed the highest degree of hydrolysis (DH) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activities. Results: The optimized conditions were as follows: water/material ratio of 5.4:1, a time span of 5 h, pH of 7.0, enzyme concentration of 2000 U/g, and temperature of 45 °C; the maximum DPPH scavenging activity and DH were 92.861% and 19.302%, respectively. LPs exhibited appreciable antioxidant activities, including DPPH radical, hydroxyl radical, 2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonate (ABTS) radical, and superoxide anion scavenging activities. LPs attenuated H2O2-related oxidative injury in RAW264.7 cells, reduced the reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and increased the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) levels. Conclusion: We concluded that LPs could be an ideal source of bioactive peptides from monkfish and also have pharmaceutical potential.

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Oral Intake of Collagen and Collagen Hydrolysate From Takifugu bimaculatus Attenuates Ultraviolet‐Induced Skin Photoaging in Mice

Chen,

Lin,

Yang

et al. 2024

Food Science & Nutrition

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With the rapid emergence of pufferfish aquaculture and processing industries, fish skin is underutilized as a byproduct of processing, leading to resource waste. In this study, Takifugu bimaculatus skin collagen (TBSC) was extracted by acetic acid solubilization and its physicochemical properties were analyzed. The effects of TBSC and the TBSC hydrolysate (TBSCH) on ultraviolet (UV) irradiation‐induced photoaging were investigated using a mouse model. The purity of TBSC was 90.02%. Electrophoresis and Fourier infrared spectroscopy characterization of TBSC showed that the type of collagen in TBSC was typical standard type I. The degree of hydrolysis was selected to optimize the hydrolysis conditions for TBSC. The papain enzyme dosage, temperature, pH, and hydrolysis duration of 51,000 U/g, 48.03°C, 5.35, and 4 h have been demonstrated to be the optimum hydrolysis conditions for TBSCH. Oral administration of either TBSC or TBSCH ameliorated UV‐induced skin erythema and hyperkeratosis. TBSC and TBSCH treatment increased collagen content and had an inhibitory effect on matrix metalloproteinase (MMP)‐2 and MMP‐3 expression, whereas MMP‐9 expression was significantly reversed only in the TBSCH‐treated groups. The expression of the c‐Jun protein was much lower in these groups, suggesting that TBSCH had a greater alleviating effect on collagen degradation and extracellular matrix breakdown. Therefore, it is proposed that TBSCH has the potential to be used as a nutricosmetic agent with protective attributes against UV‐induced skin damage and concurrent collagen depletion.

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Physicochemical, antioxidant properties of giant croaker (Nibea japonica) swim bladders collagen and wound healing evaluation

Cited by 74 publications

References 42 publications

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

Pepsin-Soluble Collagen from the Skin of Lophius litulo: A Preliminary Study Evaluating Physicochemical, Antioxidant, and Wound Healing Properties

Optimization of Extraction of Bioactive Peptides from Monkfish (Lophius litulon) and Characterization of Their Role in H2O2-Induced Lesion

Oral Intake of Collagen and Collagen Hydrolysate From Takifugu bimaculatus Attenuates Ultraviolet‐Induced Skin Photoaging in Mice

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