Physicochemical, Bacteriological, and Radiochemical Characterization of Some Algerian Thermal Spring Waters

Amarouche-Yala, Samia; Benouadah, Ali; Bentabet, Abd el Ouahab; Moulla, Adnane Souffi; Ouarezki, Sid Ali; Algouti, Ahmed

doi:10.1007/s12403-014-0130-x

Cited by 15 publications

(1 citation statement)

References 26 publications

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“…Algerian hot springs formerly were only used as spas for balneology [22], regarding their therapeutic effects [23]. At these spots, researchers were mainly interested in geothermal potential, physicochemical composition, and bacteriological and fungal contamination investigations to ensure the safety of users [24][25][26]. Despite intensive studies on terrestrial thermal springs around the world, very little is known about the microbial biodiversity of hydrothermal springs in Algeria.…”

Section: Introductionmentioning

confidence: 99%

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme activities were also investigated. A total of 293 strains were isolated from the hot springs' water and sediment using different culture media. Overall, five distinct bacterial groups were characterized by whole-cell protein pattern analysis. Based on the 16S rRNA gene sequencing of 100 selected strains, the isolates were assigned to the following three major phyla: Firmicutes (93%), Deinococcus-Thermus (5%), and Actinobacteria (2%), which included 27 distinct species belonging to 12 different phylotypes, Aeribacillus, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, Laceyella, Meiothermus, Saccharomonospora, Thermoactinomyces, Thermobifida, and Thermus. The screening for nine extracellular enzymes showed that 65.87% of the isolates presented at least five types of enzyme activities, and 6.48% of strains combined all tested enzymes (amylase, cellulase, pectinase, esculinase, protease, gelatinase, lipase, lecithinase, and nuclease). It was found that Bacillus, Anoxybacillus, Aeribacillus, and Aneurinibacillus were the genera showing the highest activities. Likewise, the study showed an abundant and diverse thermophilic community with novel taxa presenting a promising source of thermozymes with important biotechnological applications. This study showed that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully differentiate thermophilic bacteria from Algerian hot springs.

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