Physicochemical characteristics of Ganoderma lucidum oligosaccharide and its regulatory effect on intestinal flora in vitro fermentation

Xia, Qile; Zhao, Qi; Zhu, Hua; Cao, Yan; Yang, Kai; Sun, Peilong; Cai, Ming

doi:10.1016/j.fochx.2022.100421

Cited by 12 publications

(6 citation statements)

References 40 publications

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“…Based on the reported method [ 22 ] with modifications, our experimental protocol was reviewed and approved by the Ethics Committee of Zhejiang University of Technology (Approval No. MGS20220617147).…”

Section: Methodsmentioning

confidence: 99%

In Vitro Digestion and Fermentation of Different Ethanol-Fractional Polysaccharides from Dendrobium officinale: Molecular Decomposition and Regulation on Gut Microbiota

Xu,

Zhu,

Chen

et al. 2024

Foods

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Polysaccharides from Dendrobium officinale have garnered attention for their diverse and well-documented biological activities. In this study, we isolated three ethanol-fractionated polysaccharides from Dendrobium officinale (EPDO) and investigated their digestive properties and effects on gut microbiota regulation in vitro. The results indicated that after simulating digestion in saliva, gastric, and small intestinal fluids, three EPDOs, EPDO-40, EPDO-60 and EPDO-80, with molecular weights (Mw) of 442.6, 268.3 and 50.8 kDa, respectively, could reach the large intestine with a retention rate exceeding 95%. During in vitro fermentation, the EPDOs were broken down in a “melting” manner, resulting in a decrease in their Mw. EPDO-60 degraded more rapidly than EPDO-40, likely due to its moderate Mw. After 24 h, the total production of short-chain fatty acids (SCFAs) for EPDO-60 reached 51.2 ± 1.9 mmol/L, which was higher than that of EPDO-80. Additionally, there was an increase in the relative abundance of Bacteroides, which are capable of metabolizing polysaccharides. EPDO-60 also promoted the growth of specific microbiota, including Prevotella 9 and Parabacteroides, which could potentially benefit from these polysaccharides. Most notably, by comparing the gut microbiota produced by different fermentation carbon sources, we identified the eight most differential gut microbiota specialized in polysaccharide metabolism at the genus level. Functional prediction of these eight differential genera suggested roles in controlling replication and repair, regulating metabolism, and managing genetic information transmission. This provides a new reference for elucidating the specific mechanisms by which EPDOs influence the human body. These findings offer new evidence to explain how EPDOs differ in their digestive properties and contribute to the establishment of a healthy gut microbiota environment in the human body.

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Section: Methodsmentioning

confidence: 99%

In Vitro Digestion and Fermentation of Different Ethanol-Fractional Polysaccharides from Dendrobium officinale: Molecular Decomposition and Regulation on Gut Microbiota

Xu,

Zhu,

Chen

et al. 2024

Foods

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“…The strongly polar DB–FFAP column (0.32 mm × 30 m × 0.5 μm, Agilent Technologies, USA) was used for the detection of SCFAs on the diluted intestinal contents. The specific instrumentation conditions can be found in our previous study …”

Section: Methodsmentioning

confidence: 99%

“…The specific instrumentation conditions can be found in our previous study. 25 2.13. Statistical Analysis.…”

Section: Preparation and Analysis Of Dopmentioning

confidence: 99%

Phenolics from Dendrobium officinale Leaf Ameliorate Dextran Sulfate Sodium-Induced Chronic Colitis by Regulating Gut Microbiota and Intestinal Barrier

Wang,

Cai,

Yang

et al. 2023

J. Agric. Food Chem.

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The increasing incidence of colitis and the side effects of its therapeutic drugs have led to the search for compounds of natural origin, including phenolics, as new treatments for colitis. In this study, the potential mechanism of Dendrobium officinale leaf phenolics (DOP) on the relief of dextran sulfate sodium (DSS)-induced colitis was explored. The results showed that DOP treatment for 36 days reduced the symptoms of colitis caused by DSS, including reduction of the disease activity index and alleviation of colonic tissue damage. In addition, DOP downregulated the expression of key proteins of the TLR4/NF-κB signaling pathway and reduced the production of inflammatory cytokines. Furthermore, DOP could enhance the expression of tight junction proteins including ZO-1, Occludin, and Claudin-1 to restore intestinal mucosal barrier function. DOP also effectively regulates disordered intestinal flora and enhances the production of short-chain fatty acids, which is also beneficial in modulating gut internal environmental homeostasis, inhibiting inflammation, and restoring the intestinal barrier. These findings indicated that DOP can ameliorate DSS-induced chronic colitis by regulating gut microbiota, intestinal barrier, and inflammation, and it is a promising ingredient from D. officinale.

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“…Kai et al (2020) two kinds of oligosaccharides (UGLS-O and BGLS-O) prepared from (crushed and unbroken) G. lucidum spore powder by water extraction and alcohol precipitation can increase the relative abundance of beneficial bacteria such as Prevotella , Faecalibacterium , Bifidobacterium and Lactobacillus , but decrease the relative abundance of Escherichia-Shigella . In addition, Xia et al (2022) extracted GLO from G. lucidum by ultrasonic-assisted enzymolysis and dextran gel electrophoresis, which can not only increase the beneficial bacteria detected by the first two groups of researchers, but also reduce the relative abundance of harmful bacteria such as Escherichia-Shigella and Dorea. Because GLO structure can not be digested and degraded in oral cavity, but can be used by gut flora in gastrointestinal tract, so GLO can be used as a component of functional food or nutritional products to improve intestinal tract and enhance immunity.…”

Section: Effects Of Different Components Of Ganoderma Lucid...mentioning

confidence: 99%

Regulatory effect of Ganoderma lucidum and its active components on gut flora in diseases

Qin,

Fang,

Zhang

et al. 2024

Front. Microbiol.

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Driven by the good developmental potential and favorable environment at this stage, Ganoderma lucidum is recognized as a precious large fungus with medicinal and nutritional health care values. Among them, polysaccharides, triterpenoids, oligosaccharides, trace elements, etc. are important bioactive components in G. lucidum. These bioactive components will have an impact on gut flora, thus alleviating diseases such as hyperglycemia, hyperlipidemia and obesity caused by gut flora disorder. While numerous studies have demonstrated the ability of G. lucidum and its active components to regulate gut flora, a systematic review of this mechanism is currently lacking. The purpose of this paper is to summarize the regulatory effects of G. lucidum and its active components on gut flora in cardiovascular, gastrointestinal and renal metabolic diseases, and summarize the research progress of G. lucidum active components in improving related diseases by regulating gut flora. Additionally, review delves into the principle by which G. lucidum and its active components can treat or assist treat diseases by regulating gut flora. The research progress of G. lucidum in intestinal tract and its potential in medicine, health food and clinical application were fully explored for researchers.

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Physicochemical characteristics of Ganoderma lucidum oligosaccharide and its regulatory effect on intestinal flora in vitro fermentation

Cited by 12 publications

References 40 publications

In Vitro Digestion and Fermentation of Different Ethanol-Fractional Polysaccharides from Dendrobium officinale: Molecular Decomposition and Regulation on Gut Microbiota

In Vitro Digestion and Fermentation of Different Ethanol-Fractional Polysaccharides from Dendrobium officinale: Molecular Decomposition and Regulation on Gut Microbiota

Phenolics from Dendrobium officinale Leaf Ameliorate Dextran Sulfate Sodium-Induced Chronic Colitis by Regulating Gut Microbiota and Intestinal Barrier

Regulatory effect of Ganoderma lucidum and its active components on gut flora in diseases

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