Physicochemical Characteristics of Polypyrrole/(Glucose oxidase)/(Prussian Blue)‐based Biosensor Modified with Ni‐ and Co‐Hexacyanoferrates

Abstract

In this work, three types of electrodes suitable for amperometric glucose biosensors were designed. One type of electrode was based on bio‐selective layer of polypyrrole/(glucose oxidase)/(Prussian Blue) (Ppy/GOx/PB) and it was used as a control electrode regarding to which electrochemical properties of two other types of electrodes were compared. During the formation of Prussian blue layers graphite electrodes were additionally modified by Ni‐hexacyanoferrate (NiHCF) and by Co‐hexacyanoferrate (CoHCF) in order to design Ppy/GOx/PB‐NiHCF and Ppy/GOx/PB‐CoHCF electrodes, respectively. Some physicochemical characteristics of all three types of electrodes were evaluated and compared. The Ppy/GOx/PB‐NiHCF electrode showed wider linear range of the calibration curve than Ppy/GOx/PB and Ppy/GOx/PB‐CoHCF electrodes. The effect of temperature on analytical performance of the Ppy/GOx/PB‐NiHCF based biosensor has been evaluated and activation energy of enzyme catalysed reaction has been calculated within the temperature range of 15 °C to 30 °C.

Synthesis and Applications of Prussian Blue and Its Analogues as Electrochemical Sensors

“…Therefore, electrochemical sensors are widely used in industrial, clinical diagnostics, food analysis and environment monitoring. For electrochemical sensor, an electrode acts the roles of transducer to measure the current generated by electrochemical oxidation or reduction on the working electrode, [51] then transferring into an electrical signal as the concentration of the analyte under study. [52] The sensor's performance is usually evaluated by the following criteria: [53] (1) Sensitivity: The definition of sensitivity is the slope of the calibration curve.…”

Increasing Electrical Conductivity of Bacterial Cellulose/Polypyrrole Bio Composite Films Prepared Using the Pulling Technique

“…However, the processability of PANI was relatively poor because it can be infused and insoluble in common solvents [13]. In contrast to PANI, polypyrrole was used as a conductive polymer because easy to synthesize, environmentally friendly, and has relatively high conductivity [14] [15]. The polymerized Ppy can be produced by chemical polymerization/electrochemical using the oxidation of the pyrrole monomer [16].…”

Prussian Blue Modified Amperometric Hg2+ Ion Biosensor Based on Glucose Oxidase Inhibition

“…Prussian blue (PB) is a well-known, inexpensive, and easily-prepared redox material often used in developing sensors and biosensors [25][26][27][28][29][30][31][32][33][34]. One of the most important features of PB, determining its wide applicability in biosensorics, is the ability of PB to catalyze electrochemical reduction of hydrogen peroxide (Eq.…”