Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex

Burga-Sánchez, Jonny; Ferreira, Luiz Eduardo Nunes; Volpato, Maria Cristina; Cabeça, Luis Fernando; Braga, Mário Antônio; Fraceto, Leonardo Fernandes; Paula, Eneida de; Groppo, Francisco Carlos

doi:10.1007/s00210-020-01879-1

Cited by 8 publications

(5 citation statements)

References 45 publications

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“…In polymeric nanocapsules and liposomes, the authors showed in vivo results of pain control using a chronic inflammation model in comparison with solutions of free ATC associated with a vasoconstrictor agent. In that case, the vasoconstrictor effect guaranteed similar anesthesia for free ATC (plus vasoconstrictor) and the nanoformulations [37]. These results show that systemic absorption of ATC can be one of the main causes of failure or decrease in anesthetic potency.…”

Section: Pharmacokinetic Study (Local Microdialysis In the Inflamed T...mentioning

confidence: 62%

“…The literature shows a couple of articles on the encapsulation of ATC in cyclodextrin [37], in polymeric nanocapsules [38], and in liposomes [39]. In polymeric nanocapsules and liposomes, the authors showed in vivo results of pain control using a chronic inflammation model in comparison with solutions of free ATC associated with a vasoconstrictor agent.…”

Section: Pharmacokinetic Study (Local Microdialysis In the Inflamed T...mentioning

confidence: 99%

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Improved Local Anesthesia at Inflamed Tissue Using the Association of Articaine and Copaiba Oil in Avocado Butter Nanostructured Lipid Carriers

et al. 2023

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Unsuccessful anesthesia often occurs under an inflammatory tissue environment, making dentistry treatment extremely painful and challenging. Articaine (ATC) is a local anesthetic used at high (4%) concentrations. Since nanopharmaceutical formulations may improve the pharmacokinetics and pharmacodynamics of drugs, we encapsulated ATC in nanostructured lipid carriers (NLCs) aiming to increase the anesthetic effect on the inflamed tissue. Moreover, the lipid nanoparticles were prepared with natural lipids (copaiba (Copaifera langsdorffii) oil and avocado (Persia gratissima) butter) that added functional activity to the nanosystem. NLC-CO-A particles (~217 nm) showed an amorphous lipid core structure according to DSC and XDR. In an inflammatory pain model induced by λ-carrageenan in rats, NLC-CO-A improved (30%) the anesthetic efficacy and prolonged anesthesia (3 h) in relation to free ATC. In a PGE2-induced pain model, the natural lipid formulation significantly reduced (~20%) the mechanical pain when compared to synthetic lipid NLC. Opioid receptors were involved in the detected analgesia effect since their blockage resulted in pain restoration. The pharmacokinetic evaluation of the inflamed tissue showed that NLC-CO-A decreased tissue ATC elimination rate (ke) by half and doubled ATC’s half-life. These results present NLC-CO-A as an innovative system to break the impasse of anesthesia failure in inflamed tissue by preventing ATC accelerated systemic removal by the inflammatory process and improving anesthesia by its association with copaiba oil.

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Section: Pharmacokinetic Study (Local Microdialysis In the Inflamed T...mentioning

confidence: 62%

Section: Pharmacokinetic Study (Local Microdialysis In the Inflamed T...mentioning

confidence: 99%

Improved Local Anesthesia at Inflamed Tissue Using the Association of Articaine and Copaiba Oil in Avocado Butter Nanostructured Lipid Carriers

et al. 2023

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“…Articaine, a widely used local anesthetics in dentistry, was shown to benefit from inclusion into cyclodextrins regarding safety: a 1:1 inclusion compound of HPβCD and articaine, prepared by co-dissolution/freeze-drying, had lower in vitro cytotoxicity of the drug against human primary gingival fibroblasts (HGF cells) than the free drug, with IC 50 values increasing from 16 μM (pure articain) to >20 μM, the highest concentration tested, with HPβCD·articaine [ 48 ]. Moreover, fluorescence microscopy of the gingival cells revealed loss of cytoplasm volume and the presence of apoptotic bodies on cells treated with 10 and 20 μM of pure articaine, which were not observed with HPβCD·articaine at the same concentrations.…”

Section: Cyclodextrins In Dental Repairmentioning

confidence: 99%

Cyclodextrins as Multi-Functional Ingredients in Dentistry

Braga

2023

Pharmaceutics

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Cyclodextrins are present in a variety of oral hygiene compositions. The present work describes the role of cyclodextrins in several toothpastes and mouthwashes that are already available in the market, as well as their prospective use in other applications as investigated in studies in the literature. Moreover, cyclodextrins are under study for the development of materials used in various techniques of dental repair, such as fillings, cements and binders therein. Their role in each of the innovative materials is presented. Finally, the prospect of the use of cyclodextrin-based delivery systems for the oral cavity is introduced, with a focus on new cyclodextrin molecules with dual action as bone-targeting agents and osteogenic drugs, and on new cross-linked cyclodextrin particles with a high drug loading and sustained drug delivery profile for the treatment of diseases that require prolonged action, such as periodontitis. In conclusion, cyclodextrins are herein demonstrated to act as versatile and multi-action ingredients with a broad range of applications in dentistry.

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“…The test sample of 5 mg was placed in an aluminum pan that had been crimped, and an empty aluminum pan was considered as a standard. During the testing process, a steady rate of 10 • C/min was applied to the DSC system during its operation at a temperature range of 50 • C to 300 • C [41].…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

Ternary Inclusion Complex of Sinapic Acid with Hydroxypropyl-β-cyclodextrin and Hydrophilic Polymer Prepared by Microwave Technology

et al. 2022

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Sinapic acid (SA) is a poorly water-soluble substance which could result in poor bioavailability. The aim of this study was to determine the “hydroxypropyl β-cyclodextrin (HPβCD)” solubilization of SA in the presence of the auxiliary substance hydroxypropyl methylcellulose (HPMC) and to evaluate the ternary inclusion complex prepared by microwave technology. Phase-solubility profiles showed that HPβCD exhibited the greatest solubilizing effect on SA in the presence of HPMC. The enhanced rate of SA dissolution was exhibited by a ternary complex. Outcomes of analyses such as “DSC, FTIR, NMR, and SEM” confirmed the embedding of SA into the cavity of the HPβCD and the formation of a ternary inclusion complex. The outcomes of antioxidant activity (ABTS and nitric oxide scavenging activity) demonstrated that SA ternary inclusion complex (TIC) presented strong antioxidant activity, which might be a result of the enhanced solubility of SA in the TIC prepared by microwave technology. Hence, SA-TIC formulation could be a better dosage form which may protect the body from free radical damage and oxidative stress. Microwave technology greatly boosted the interaction of SA with HPβCD and HPMC, and such findings are expected to contribute to raising the solubility of SA, thereby improving the bioavailability of SA.

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Physicochemical characterization and cytotoxicity of articaine-2-hydroxypropyl-β-cyclodextrin inclusion complex

Cited by 8 publications

References 45 publications

Improved Local Anesthesia at Inflamed Tissue Using the Association of Articaine and Copaiba Oil in Avocado Butter Nanostructured Lipid Carriers

Improved Local Anesthesia at Inflamed Tissue Using the Association of Articaine and Copaiba Oil in Avocado Butter Nanostructured Lipid Carriers

Cyclodextrins as Multi-Functional Ingredients in Dentistry

Ternary Inclusion Complex of Sinapic Acid with Hydroxypropyl-β-cyclodextrin and Hydrophilic Polymer Prepared by Microwave Technology

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