Physicochemical characterization of a thermostable glycoprotein bioemulsifier from Solibacillus silvestris AM1

Markande, Anoop R.; Acharya, Srijan; Nerurkar, Anuradha S.

doi:10.1016/j.procbio.2013.08.017

Cited by 52 publications

(41 citation statements)

References 46 publications

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“…A summary of such ndings is shown in Table 1, along with their identied bioemulsier compositions. Bacterial trains belonging to genera including Acinetobacter, 12 Aeribacillus, 13 Alcaligenes, 14 Amycolatopsis, 15 Azotobacter, 16 Bacillus, 17 Beijerinckia, 18 Corynebacterium, 19 Enterobacter, 20 Geobacillus, 13 Halomonas, 21 Klebsiella, 22 Myroides, 23 Pedobacter, 24 Propionibacterium, 25 Pseudomonas, 26 Solibacillus, 27 Streptomyces, 28 Variovorax, 29 have been reported to be bioemulsier producers (Table 1). To date, however, no strains belong to the genus of Exiguobacterium have been reported as bioemulsier producers.…”

Section: Introductionmentioning

confidence: 99%

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

et al. 2017

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In this study, Exiguobacterium N4-1P is reported as a bioemulsifier producer for the first time. The strain was found to be closely related to E. oxidotolerans strain T-2-2 T , E. antarcticum B7 T , and E. antarcticum B7 T with similarities of 99.47, 98.70 and 98.63%, respectively. Its phenotypic properties such as metabolic fingerprints, membrane composition, and cell morphology were determined. Different carbon sources were used for bioemulsifier production and diesel was confirmed to stimulate the yield effectively. The produced bioemulsifier is a complex mainly composed of lipopeptides with C16:0 (32.18%) and C18:0 (40.99%) as the primary fatty acids. The produced bioemulsifier could form emulsions effectively with diverse hydrocarbons. No foams were formed during the production and applications, which would facilitate its commercialization. The bioemulsifier was stable over a wide range of salinity (0-25%), pH (2-12), and temperature (below 50 C). Exiguobacterium N4-1P and the produced bioemulsifier fills knowledge gaps and has promising application potential in diverse fields, especially in environmental engineering.

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Section: Introductionmentioning

confidence: 99%

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

et al. 2017

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“…Isolates were inoculated into 50 ml Zobell Marine Broth in 250-ml Erlenmeyer flasks and incubated at 30°C for 72 h. After incubation, 1:1 of the cell-free supernatant of culture broth and paraffin oil were mixed using the homogenizer (REMI RQT 127A) for 3-5 min at 12,000 rpm and the emulsion formed was observed for 72 h. After every 24 h, the emulsification index (%EI) of the 24 h (E 24 , E 48 , and E 72 ) was calculated using the modified formula (Cooper and Goldenberg 1987) reported by Markande et al (2013).…”

Section: Emulsification Index (%Ei)mentioning

confidence: 99%

“…The bioemulsifier has been previously studied by us (Markande et al 2013). Fifty milliliters of cell-free supernatant of 48 h grown selected isolate was mixed with paraffin oil (1:1) and kept overnight.…”

Section: Partial Purification Of the Bioemulsifiermentioning

confidence: 99%

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Microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the Indian intertidal zone

Markande

Nerurkar

2016

Environ Sci Pollut Res

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Isolates were obtained from intertidal zone site samples from all five western and one eastern coastal states of India and were screened. These ecophysiological groups of aerobic, mesophilic, heterotrophic, sporulating, and bioemulsifier-producing bacteria were from Planococcaceae and Bacillaceae. This is the first report of bioemulsifier production by Sporosarcina spp., Lysinibacillus spp., B. thuringiensis, and B. flexus. In this group, Solibacillus silvestris AM1 was found to produce the highest emulsification activity (62.5 %EI) and the sample that yielded it was used to isolate the ecophysiological group of non-bioemulsifier-producing, hydrocarbon-degrading bacteria (belonging to Chromatiales and Bacillales). These yielded hitherto unreported degrader, Rheinheimera sp. CO6 which was selected for the interaction studies (in a microcosm) with bioemulsifier-producing S. silvestris AM1. The gas chromatographic study of these microcosm experiments revealed increased degradation of benzene, toluene, and xylene (BTX) and the growth of Rheinheimera sp. CO6 in the presence of bioemulsifier produced by S. silvestris AM1. Enhancement of the growth of S. silvestris AM1 in the presence of Rheinheimera sp. CO6 was observed possibly due to reduced toxicity of BTX suggesting mutualistic association between the two. This study elucidates the presence and interaction between enhancers and degraders in a hydrocarbon-contaminated intertidal zone and contributes to the knowledge during application of the two in remediation processes.

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“…The cultures were centrifuged (8000 × g) for 10 min and cell-free supernatants were checked for the reduction in the surface tension at room temperature. Emulsification index (% EI) of the supernatants was calculated by the method described by Markande et al [27] using the formula…”

Section: Assay For Surfactant Tolerance and Productionmentioning

confidence: 99%

Analysis of Midgut Bacterial Community Structure of <i>Neanthes chilkaensis</i> from Polluted Mudflats of Gorai, Mumbai, India

Markande¹,

Mikaelyan²,

Nayak³

et al. 2014

AiM

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Adaptation of microbial communities to anthropogenic stress has often been shown to involve reduction in diversity and in selective enrichment of species capable of survival. The gut bacterial community of pollution-stressed polychaete Neanthes chilkaensis was studied using cultivation dependent and independent approaches. The possible role of the gut bacteria in remediation of pollutants was also studied. The analysis of clones covering about 90% of the 16S rRNA clone library indicated that the majority of the taxa in the library resemble GenBank entries of aromatichydrocarbon pollutant degraders. Among them Firmicutes and Actinobacteria were dominant. About 84% of the gut isolates were capable of utilizing PAHs as sole carbon sources while 20% of isolates were found to produce biosurfactants. Production of surfactant along with capability to utilize PAH and other pollutants indicate the role of the gut community in alleviating the pollution stress. The results also show the potential source of microorganisms with industrial and environmental application.

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Physicochemical characterization of a thermostable glycoprotein bioemulsifier from Solibacillus silvestris AM1

Cited by 52 publications

References 46 publications

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

Microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the Indian intertidal zone

Analysis of Midgut Bacterial Community Structure of <i>Neanthes chilkaensis</i> from Polluted Mudflats of Gorai, Mumbai, India

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Physicochemical characterization of a thermostable glycoprotein bioemulsifier from Solibacillus silvestris AM1

Cited by 52 publications

References 46 publications

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

Microcosm-based interaction studies between members of two ecophysiological groups of bioemulsifier producer and a hydrocarbon degrader from the Indian intertidal zone

Analysis of Midgut Bacterial Community Structure of &lt;i&gt;Neanthes chilkaensis&lt;/i&gt; from Polluted Mudflats of Gorai, Mumbai, India

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Analysis of Midgut Bacterial Community Structure of <i>Neanthes chilkaensis</i> from Polluted Mudflats of Gorai, Mumbai, India