Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell

Yang, Ke; Lu, Tingting; Zhan, Lianghui; Zhou, Cong; Zhang, Ning-Yu; Lei, Shanshan; Wang, Yuzhi; Yang, Junsheng; Yan, Ming; Lv, Gui‐Yuan; Chen, Suhong

doi:10.1016/j.ijbiomac.2020.01.026

Cited by 49 publications

(29 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 1, LDOP was a heteropolysaccharide comprising mannose, galacturonic acid, glucose, galactose, and arabinose at a molar ratio of 2.0:1.1:0.7:0.5:0.4. Next, we got LDOP-1 for subsequent experiments by our previous plan (Yang et al, 2020).…”

Section: Hplc Analysis Of Ldop Monosaccharide Compositionmentioning

confidence: 99%

Polysaccharides of Dendrobium officinale Kimura & Migo Leaves Protect Against Ethanol-Induced Gastric Mucosal Injury via the AMPK/mTOR Signaling Pathway in Vitro and vivo

Zhan

Lü

et al. 2020

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

Ethanol-induced gastric mucosal injury is a common gastrointestinal disorder. Polysaccharides separated from herbs have been shown to be effective for ethanol-induced gastric mucosal injury, but whether the polysaccharides from Dendrobium officinale Kimura & Migo leaves (LDOP-1) protected mucosa from ethanol-induced injury remains unknown. Thus, the present study carried out gastric mucosal protection and the mechanism of LDOP-1 in vivo and vitro . The chemical composition of LDOP-1 was a heteropolysaccharide comprising mannose, galacturonic acid, glucose, galactose, and arabinose at a molar ratio of 2.0:1.1:0.7:0.5:0.4. Pharmacological results showed that LDOP-1 significantly reduced gastric mucosal injury score and pathological injury, improved antioxidant capacity, reduced the level of reactive oxygen species, and reversed the apoptosis of GES-1 in vivo and vitro . Research showed that LDOP-1 pretreatment upregulated the expression level of p-AMPK, LC3β, HO-1, and Beclin-1; downregulated the expression level of p-mTOR and p62; and reversed the expression level of caspase3, Bax, and Bcl-2. This study was the first to demonstrate that LDOP-1 could protect against ethanol-induced gastric mucosal injury via the AMPK/mTOR signaling pathway in vitro and vivo .

show abstract

Section: Hplc Analysis Of Ldop Monosaccharide Compositionmentioning

confidence: 99%

Polysaccharides of Dendrobium officinale Kimura & Migo Leaves Protect Against Ethanol-Induced Gastric Mucosal Injury via the AMPK/mTOR Signaling Pathway in Vitro and vivo

Zhan

Lü

et al. 2020

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Polysaccharides are the main medicinal components of D. officinale, with a wide range of medicinal properties. A large number of polysaccharides have been isolated from D. officinale, including DWDOP1, DWDOP2, DWDOP3, FWDOP1, FWDOP2, FWDOP3 (Yu et al, 2018), DOP-50, DOP-60, DOP-70 (Xing et al, 2018), DO (Ma et al, 2018), DOPA-1 (Wei et al, 2018), DOP1-DES, DOP2-DES , LDOP-1 (Yang et al, 2020), UDP-1, FLP-1, and FDP-1 (Liang K. L et al, 2018), which are identified by various analytical technologies, such as high performance liquid chromatography (HPLC), high performance gel permeation chromatography (HPAEC), gas chromatography-mass spectrometry (GC-MS), and gel permeation chromatography (GPC). The molecular weights of D. officinale polysaccharides range from 30 to 1,415 kDa Ma et al, 2018;Wei et al, 2018;Yu et al, 2018).…”

Section: Polysaccharidesmentioning

confidence: 99%

Traditional Uses, Phytochemistry, Pharmacology, and Quality Control of Dendrobium officinale Kimura et. Migo

Wen-hua

Zhang

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Dendrobium officinale, a well-known plant used as a medicinal and food homologous product, has been reported to contain various bioactive components, such as polysaccharides, bibenzyls, phenanthrenes, and flavonoids. It is also widely used as a traditional medicine to strengthen “Yin”, nourish heart, tonify five viscera, remove arthralgia, relieve fatigue, thicken stomach, lighten body, and prolong life span. These traditional applications are in consistent with modern pharmacological studies, which have demonstrated that D. officinale exhibits various biological functions, such as cardioprotective, anti-tumor, gastrointestinal protective, anti-diabetes, immunomodulatory, anti-aging, and anti-osteoporosis effects. In this review, we summarize the research progress of D. officinale from November 2016 to May 2021 and aim to better understand the botany, traditional use, phytochemistry, and pharmacology of D. officinale, as well as its quality control and safety. This work presents the development status of D. officinale, analyzes gaps in the current research on D. officinale, and raises the corresponding solutions to provide references and potential directions for further studies of D. officinale.

show abstract

“…Moreover, the leaves exhibit potent antioxidant activity, and, in addition, antihyperlipidemia, antihypertensive, antihyperuricemia, anti-inflammatory, antimicrobial, cytotoxic and antitumor, hepatoprotective, immunomodulatory, lipase-inhibitory, and tyrosinase-inhibitory Metabolites 2021, 11, 351 2 of 17 activities have been reported [8,9]. Therefore, D. catenatum leaves offer broad prospects for the development of new functional foods and were listed as a new raw food ingredient by the State Health Planning Commission of China in January 2017 [10]. Increasing interest in the nutritional and pharmacological properties of D. catenatum leaves motivated our investigation into their phytochemical composition, as well as the various manufacturing processes used to develop functional foods.…”

Section: Introductionmentioning

confidence: 99%

Effects of Various Processing Methods on the Metabolic Profile and Antioxidant Activity of Dendrobium catenatum Lindley Leaves

Liu

Zhang

et al. 2021

Metabolites

View full text Add to dashboard Cite

The metabolite profiles and antioxidant activity of Dendrobium catenatum Lindley leaf, a new functional ingredient for food product development, were evaluated in samples that had been prepared using various methods, including freeze-drying, hot-air drying, rolling before drying, steaming before drying, steaming and rolling before drying, and drying at 100, 80, and 60 °C. The concentrations of polysaccharides and flavonoids, as well as the antioxidant capacity of each sample, were determined. Furthermore, two nucleosides, four amino acids, one monoaromatic compound, and eight flavonoids were identified in dried leaves using high-performance liquid chromatography–diode array detector–electrospray ionization–multistage mass spectrometry (HPLC-DAD-ESI-MSn) and ultraviolet (UV) spectral analyses. The content of polar compounds such as cytidylic acid, arginine, tyrosine, and hydroxybenzoic acid hexose increased dramatically during hot-air-drying and rolling-before-drying treatments, while flavonol C-glycosides remained stable throughout the various treatments and drying temperatures. Rolling before drying at 100 °C was identified as the most suitable process when manufacturing tea products from D. catenatum leaves. This process resulted in a high-antioxidant-activity and visually appealing tea. This report details a potential strategy that should be applied in the manufacturing processes of high-quality products from D. catenatum leaves.

show abstract

Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell

Cited by 49 publications

References 19 publications

Polysaccharides of Dendrobium officinale Kimura & Migo Leaves Protect Against Ethanol-Induced Gastric Mucosal Injury via the AMPK/mTOR Signaling Pathway in Vitro and vivo

Polysaccharides of Dendrobium officinale Kimura & Migo Leaves Protect Against Ethanol-Induced Gastric Mucosal Injury via the AMPK/mTOR Signaling Pathway in Vitro and vivo

Traditional Uses, Phytochemistry, Pharmacology, and Quality Control of Dendrobium officinale Kimura et. Migo

Effects of Various Processing Methods on the Metabolic Profile and Antioxidant Activity of Dendrobium catenatum Lindley Leaves

Contact Info

Product

Resources

About