Plant lipid biomarkers, such as plant waxes and terpenoids, and the stable isotopic composition of bulk leaves are widely used in both modern and paleoclimate studies for tracking vegetation and climate. However, the effects of different drying methods on the preservation of plant lipid biomarkers and the stable isotopic compositions of leaves are less explored. Here, we investigated various drying methods for the measurement of plant lipid concentrations and bulk leaf isotopic compositions. Methods: Leaves from four tree species (Acer rubrum, Pinus sylvestris, Platanus occidentalis, and Taxodium distichum) were collected and dried using air, an oven, a freeze-dryer, and a microwave. We compared concentrations of leaf waxes and terpenoids and carbon (δ 13 C) and nitrogen (δ 15 N) isotopic compositions of leaves by different drying methods. Results: The air, oven, freeze-dryer, and microwave drying methods did not affect lipid concentrations significantly, and only a few homologues differed (38.1% or 41.8 μg/g on average) possibly due to biological variations or enhanced extraction efficiencies. The δ 13 C values were not affected by drying methods, whereas the δ 15 N values in oven-dried leaves in some species were higher by 0.2-0.7‰ than those obtained by other methods. Though small, we attribute these patterns to loss of leaf compounds with lower isotope ratios during oven-drying. Conclusions: Based on our results, each drying technique yielded equivalent results for all plant wax and terpenoid concentrations and bulk leaf δ 13 C values; however, oven-drying modified the δ 15 N values. 1 | INTRODUCTION Molecular biomarkers of terrestrial plants such as leaf waxes and terpenoids are commonly used to infer vegetation and climate. 1,2 When these biomarkers are well preserved without significant degradation on geologic timescales, they can provide continuous, high-resolution records of the past environmental change. 3-6 Plant waxes consist of long-chain n-alkanes (C 25-C 35), n-alkanoic acids (C 26-C 36), n-alkanols (C 26-C 36), and wax esters, and provide protection against dehydration, pathogens, insects, and ultraviolet light exposure. 7-9 These n-alkyl compounds have characteristic carbon chain length distribution patterns and concentrations that can be unique among growth forms and major taxonomic groups. 10-13 For example, n-alkanes from terrestrial plants exhibit odd-over-even carbon chain predominance and longer chains (i.e. n-C 33 and n-C 35) are produced typically in higher amounts in grasses than in trees. 10,11 Terpenoids are another important class of plant biomarkers, which are often used as major plant taxon indicators for angiosperms and gymnosperms. 14,15 For example, the non-steroidal pentacyclic triterpenoids and tricyclic diterpenoids are produced by angiosperms and gymnosperms, respectively. The ratio of diterpenoid to n-alkanes has been suggested as an indicator of paleovegetation composition. 16 Utilizing the amount and ratios of molecular biomarkers in modern calibration studies and paleo-en...