Physicochemical, Phytochemical and High-Performance Thin Layer Chromatography Analysis of the Root Barks of Onosma Echioides

Shoaib, Ambreen; Siddiqui, Hefazat Hussain; Badruddeen, Badruddeen; Rizvi, Aleza; Dixit, Rakesh

doi:10.22159/ajpcr.2017.v10i10.20064

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…The chromatograms were scanned by a CAMAG TLC Scanner after spraying with respective spray reagents and dried at 100° in a hot air oven. The peak number with its height, area and R f values of fingerprint data were recorded by WIN CATS (1.3.4 version) software [9][10][11][12][13][14].…”

Section: Paper Chromatographymentioning

confidence: 99%

Isolation and High-Performance Thin-Layer Chromatography Analysis of Conditional Amino Acids From the Fresh Leaves of Alternanthera Sessilis

Swarnakumari

Mohan

et al. 2019

Asian J Pharm Clin Res

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Objective: The objective of the study was to isolate the conditional amino acids present in the fresh leaves extracts of Alternanthera sessilis and compare the isolated amino acids with amino acid standards. Method: The fresh plant material was used for extraction. For the extracted sample solution, paper chromatography and high-performance thin-layer chromatography (HPTLC) fingerprinting analysis were then carried out. Results: Since A. sessilis is rich in protein, various amino acids have been isolated in aggregates and paper chromatography has been performed. Amino acids were separated and identified using trial and error method. Cysteine, glycine, aspartic acid, and proline are the standard amino acids used for comparing with the isolated amino acids by HPTLC fingerprint analysis, which gives an idea for the authentication of the plant, its constituents and also provides a parameter for quality control. Conclusion: The results scientifically validate the use of A. sessilis in the traditional medicine and also as a food.

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Section: Paper Chromatographymentioning

confidence: 99%

Isolation and High-Performance Thin-Layer Chromatography Analysis of Conditional Amino Acids From the Fresh Leaves of Alternanthera Sessilis

Swarnakumari

Mohan

et al. 2019

Asian J Pharm Clin Res

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“…The results of accuracy and precision testing showed that the methods had met the accuracy and precision requirements (% UPK, 98-102%, and % KV, ≤2.0%) [10,12,13].…”

Section: Dyta Et Almentioning

confidence: 99%

Analysis of Disodium 5’-Guanylate and Disodium 5’-Inosinate as Flavor Enhancer in Food Spices by Thin-Layer Chromatography–densitometry

Dyta

Harmita

2018

Int J App Pharm

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Objective: The objective of this study was to obtain an optimum and valid method of analysis to determine the levels of disodium 5'-guanylate (DSG) and disodium 5'-inosinate (DSI) in six samples of spices. Methods:The optimum method was obtained using silica gel 60 F 254 as the stationary phase and isopropanol:water:25% ammonia at a ratio of 6:3:1 (v/v) as the mobile phase. The developed spots were scanned using a densitometer in absorbance mode at 260 nm. The methods were valid based on the accuracy criteria (DSG, 99.11-99.96%, and DSI, 98.56-101.05%), precision (DSG, 1.09%, and DSI, 0.49%), and linearity (DSG, r=0.9909, and DSI, r=0.9976). Results:The results showed that the levels of DSG in samples A, B, C, D, E, and F were 0.70%, 0.79%, 0.78%, 0.99%, 1.08%, and 1.08% and those of DSI were 0.66%, 0.74%, 0.71%, 0.66%, 0.54%, and 0.67%, respectively. Conclusion:The optimum conditions of DSG and DSI for thin-layer chromatography-densitometry were obtained with silica gel 60 F 254 as the stationary phase, isopropanol:water:25% ammonia (6:3:1) as the mobile phase, and a maximum wavelength of about 260 nm. Validation results indicated that the accuracy of the analytical method for DSG was about 99.11-99.96% with a coefficient variation (precision) of 0.70-1.41%, while that for DSI was 98.56-101.05% with a coefficient variation of 0.23-0.75%. The correlation coefficients for the analytical method for DSG and DSI were 0.9909 and 0.9976, respectively. The results determined that the levels of DSG and DSI in samples A, B, C, D, E, and F were 0.70%/0.60%, 0.79%/0.74%, 0.78%/0.71%, 0.99%/0.66%; 1.08%/0.54%, and 1.08%/0.67%, respectively.

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“…Mainly the root extracts of O. echioides are applied as antiseptic for skin injuries, contusion and eruptions. The two main active components namely shikonin and alkannin (enantiomeric natural dyes) along with their oligomers is present in O. echioides root bark (SHOAIB et al, 2017). Shikonin is the major component and possess wound healing, anti-cancer activity and has shown apoptosis and cell cycle arrest in MDA-MB-231 and HCT-116 cancer cell lines (Barreto et al, Kumar et 475 al., 2007, Kumar et al, 2013.…”

Section: Introductionmentioning

confidence: 99%

Phytosomes of Naphthoquinone enriched Extract of Root bark of Onosma echioides exhibit wound healing activity in Rats

Pananchery¹,

Gadgoli²

2021

Indonesian J Pharm

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The present study is aimed at evaluation of phytosomal gel of the petroleum ether extract of root bark of Onosma echiodes for wound healing activity in rats. Extract of root bark of O. echioides was standardized by isolated naphthoquinone dimer using HPTLC. Phytosomes (equivalent to 2% w/w of naphthoquinones) of the standardized extract were prepared by thin film hydration technique. The wound healing efficacy of the formulation was evaluated in rats by inflicting excision and incision wounds followed by treatment of the wounds topically. The parameters evaluated for healing included determination of breaking strength and tensile strength of healed skin for incision model and percentage wound contraction, hydroxyproline content, granulation tissue free radicals and catalase in excision wound model. The formulation treated group showed a significant healing (p<0.005) of both the excision and incision wounds with respect to wound contraction and tensile strength respectively, as compared to vehicle treated group. The oxidative stress of the granulation tissue was also found to be reduced as indicated by reduced lipid peroxidation and increase in catalase activity. The phytosomal gel of O. echioides effectively exhibited wound healing effect.

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Physicochemical, Phytochemical and High-Performance Thin Layer Chromatography Analysis of the Root Barks of Onosma Echioides

Cited by 4 publications

References 5 publications

Isolation and High-Performance Thin-Layer Chromatography Analysis of Conditional Amino Acids From the Fresh Leaves of Alternanthera Sessilis

Isolation and High-Performance Thin-Layer Chromatography Analysis of Conditional Amino Acids From the Fresh Leaves of Alternanthera Sessilis

Analysis of Disodium 5’-Guanylate and Disodium 5’-Inosinate as Flavor Enhancer in Food Spices by Thin-Layer Chromatography–densitometry

Phytosomes of Naphthoquinone enriched Extract of Root bark of Onosma echioides exhibit wound healing activity in Rats

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