Physicochemical Pretreatment of Vietnamosasa pusilla for Bioethanol and Xylitol Production

Abstract

The consumption of fossil fuels has resulted in severe environmental consequences, including greenhouse gas emissions and climate change. Therefore, transitioning to alternative energy sources, such as cellulosic ethanol, is a promising strategy for reducing environmental impacts and promoting sustainable low-carbon energy. Vietnamosasa pusilla, an invasive weed, has been recognized as a high potential feedstock for sugar-based biorefineries due to its high total carbohydrate content, including glucan (48.1 ± 0.3%) and xylan (19.2 ± 0.4%). This study aimed to examine the impact of NaOH pretreatment-assisted autoclaving on V. pusilla feedstock. The V. pusilla enzymatic hydrolysate was used as a substrate for bioethanol and xylitol synthesis. After treating the feedstock with varying concentrations of NaOH at different temperatures, the glucose and xylose recovery yields were substantially higher than those of the untreated material. The hydrolysate generated by enzymatic hydrolysis was fermented into bioethanol using Saccharomyces cerevisiae TISTR 5339. The liquid byproduct of ethanol production was utilized by Candida tropicalis TISTR 5171 to generate xylitol. The results of this study indicate that the six- and five-carbon sugars of V. pusilla biomass have great potential for the production of two value-added products (bioethanol and xylitol).

Phosphoric Acid Pre-treatment Improves Enzymatic Hydrolysis of Weedy Biomass (<em>Thysanolaena latifolia</em>) for Bioethanol Production

“…"Yeast cell growth was monitored using a UV spectrophotometer (SP-830 Plus; Metertech, Taipei, Taiwan), and the optical density was measured at 600 nm. An optical density of 1.0 (at 600 nm) corresponds to approximately 1.5 × 10 7 cells/mL" [37].…”