Physicochemical profiling of drug candidates using capillary-based techniques

Jensen, Henrik; Larsen, Susan Weng; Larsen, Claus; Østergaard, Jesper

doi:10.1016/s1773-2247(13)50050-5

Cited by 8 publications

(2 citation statements)

References 145 publications

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“…This format of CE is called as capillary electrophoresis/frontal analysis (CE/FA); the height of a plateau signal is used for the quantification of the unbound species, and CE/FA has been utilized for the equilibrium or affinity analysis. 1−4 Binding equilibria of drug-protein complexes, 1,5,6 peptide complexes, 2 liposome-analyte interactions, 3 and Alkaloid-DNA complexes 7 have been investigated by CE/FA. Inhibitors of protein-protein interaction were also investigated by CE/FA.…”

Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis/Dynamic Frontal Analysis for the Enzyme Assay of 4-Nitrophenyl Phosphate with Alkaline Phosphatase

2020

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A substrate of 4-nitrophenyl phosphate was enzymatically hydrolyzed by alkaline phosphatase (ALP) in a capillary tube, while an injected zone of the substrate was electrophoretically migrating in the separation buffer containing the enzyme by capillary electrophoresis (CE). During the CE migration of the substrate from the start time of the electrophoresis to the detection time of the substrate, the substrate was continuously hydrolyzed by ALP to form a product of 4-nitrophenolate, and a plateau signal of 4-nitrophenolate was detected as a result of the zero-order kinetic reaction. The height of the plateau signal was directly related with the reaction rate, and it was used for the determination of a Michaelis-Menten constant through Lineweaver-Burk plots. Since the plateau signal is attributed to the dynamic formation of the product by the enzymatic reaction in CE, this analysis method is named as capillary electrophoresis/dynamic frontal analysis (CE/DFA). In CE/DFA, the CE separation is included on detecting the plateau signal, and the hydrolysis product before the sample injection is resolved from the dynamically and continuously formed product. The inhibition of the enzyme with the product is also eliminated in CE/DFA by the CE separation.

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Section: Introductionmentioning

confidence: 99%

Capillary Electrophoresis/Dynamic Frontal Analysis for the Enzyme Assay of 4-Nitrophenyl Phosphate with Alkaline Phosphatase

2020

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“…TDA can also be used to study substrate/ligand interactions at equilibrium, when the two entities in the interaction are in fast exchange at the time scale of the analysis. Due to fast kinetics of exchange, taylorgram is a simple Gaussian peak, which can be fitted to determine the binding constant by monitoring the change in diffusivity (or size) according to the ligand concentration introduced into the capillary. − …”

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confidence: 99%

Antigen-Adjuvant Interactions in Vaccines by Taylor Dispersion Analysis: Size Characterization and Binding Parameters

et al. 2021

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Vaccine adjuvants are immunostimulatory substances used to improve and modulate the immune response induced by antigens. A better understanding of the antigenadjuvant interactions is necessary to develop future effective vaccine. In this study, Taylor dispersion analysis (TDA) was successfully implemented to characterize the interactions between a polymeric adjuvant (poly(acrylic acid), SPA09) and a vaccine antigen in development for the treatment of Staphylococcus aureus. TDA allowed to rapidly determine both (i) the size of the antigen-adjuvant complexes under physiological conditions, and (ii) the percentage of free antigen in the adjuvant/antigen mixture at equilibrium, and finally get the interaction parameters (stoichiometry and binding constant). The complex sizes obtained by TDA were compared to the results obtained by transmission electron microscopy (TEM) and the binding parameters were compared to results previously obtained by frontal analysis continuous capillary electrophoresis (FACCE).

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Capillary-Based Techniques for Physical-Chemical Characterization of Drug Substances and Drug Delivery Systems

Østergaard

Larsen

Jensen

2016

Advances in Delivery Science and Technology

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Physicochemical profiling of drug candidates using capillary-based techniques

Cited by 8 publications

References 145 publications

Capillary Electrophoresis/Dynamic Frontal Analysis for the Enzyme Assay of 4-Nitrophenyl Phosphate with Alkaline Phosphatase

Capillary Electrophoresis/Dynamic Frontal Analysis for the Enzyme Assay of 4-Nitrophenyl Phosphate with Alkaline Phosphatase

Antigen-Adjuvant Interactions in Vaccines by Taylor Dispersion Analysis: Size Characterization and Binding Parameters

Capillary-Based Techniques for Physical-Chemical Characterization of Drug Substances and Drug Delivery Systems

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