Physicochemical properties and blood compatibility of acylated chitosan nanoparticles

Lee, Dongwon; Powers, Kevin; Baney, Ronald H.

doi:10.1016/j.carbpol.2004.06.033

Cited by 144 publications

(72 citation statements)

References 26 publications

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“…Similarly, other authors have also concluded that the charge of chitosan/pDNA complexes strongly depends on the pH and salt content of the suspension medium (Lee et al, 2004;Nimesh et al, 2010).…”

Section: Resultsmentioning

confidence: 61%

“…Due to these conflicting results, most of the research groups support the theory that the final size of the polyplex is not as important as the zeta potential on the stability of suspensions, on the adhesion of particle systems onto biological surfaces and therefore on the transfection efficiency. Consequently, we suggest that the investigation of the zeta potential is the main issue in the design of efficient nonviral vectors for gene delivery purposes, being necessary a positive zeta potential for an efficient in vitro transfection (Lee et al, 2004;Duceppe & Trabrizian, 2010).…”

Section: Resultsmentioning

confidence: 99%

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Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

et al. 2014

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Context: Ultrapure oligochitosans (UOCs) have recently been reported as efficient nonviral vectors for corneal and retinal gene delivery. However, the influence of some physicochemical factors on the transfection efficiency, such as the pH, remains unclear. Deeper in vitro research of these factors could provide valuable information for future clinical applications. Objective: The aim of this study is to determine the influence of the pH decrease on the transfection efficiency of UOC/pDNA polyplexes in HEK293 and ARPE19 cells. Materials and methods: We elaborated self-assembled UOC/pCMS-EGFP polyplexes. The influence of the most important factors on the particle size and the zeta potential was studied by an orthogonal experimental design. We evaluated, in vitro, the cellular uptake and the transfection efficiency by flow cytometry, and the cytotoxicity of the vectors by CCK-8 assay.Results and discussion: The pH of the medium strongly influences the physicochemical properties of the polyplexes, and by its modulation we are able to control their superficial charge. A significant increase on the cellular uptake and transfection efficiency of UOCs was obtained when the pH was acidified. Neither of our UOC/pCMS-EGFP polyplexes caused cytotoxicity; however, cells treated with Lipofectamine 2000ä showed decreased cell viability. Conclusion: This kind of UOC vectors could be useful to transfect cells that are in an acidic environment, such as tumor cells. However, additional in vivo studies may be required in order to obtain an effective and safe medicine for nonviral gene therapy purpose.

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Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

et al. 2014

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“…Then, 20 uL of 0.2 mol/L CaCl 2 solution was added. TEG curve was recorded using computer 9 , whose the main indicators were 1) reaction time (R): the time from the initiation of blood coagulation to the formation of fibrinogen, mainly reflecting the quality and quantity of coagulation factors; 2) coagulation time (K): the time to reach a 20 mm amplitude of thrombus from the beginning of blood coagulation; 3) Angle α: reflecting blood clotting speed; 4) maximum amplitude (MA): reflecting the maximum intensity of thrombosis 10,11 .…”

Section: ) Thromboelastography Testmentioning

confidence: 99%

Effect of Grafted Chain Length on Hemostatic Activity of N-alkylated Chitosan

Liu¹,

Yang²,

Guan³

et al. 2018

Proceedings of the 4th Annual International Conference on Material Engineering and Application (ICMEA 2017)

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Abstract-This study aimed to synthesize N-alkylated chitosan derivates with similar degree of substitution (DS) and alkyl groups of different carbon chain lengths via reductive alkylation, and then to compare their coagulation properties. Structural properties of the N-alkylated chitosan derivates were characterized by Fourier transform infrared spectroscopy, elemental analysis and 1 H nuclear magnetic resonance spectroscopy. Their pro-coagulant properties were evaluated by detecting whole blood clotting time and performing TEG test. Pro-coagulant mechanisms of the Nalkylated chitosan derivates were explored by detecting intracellular Ca 2+ concentration in platelets and P-selectin expression on platelets. We successfully prepared N-alkylated chitosan derivates with a DS of 32.88%-39.78%, including NACS6, NACS12 and NACS18. Our experimental findings showed that all N-alkylated chitosan derivates (NACS6, NACS12, NACS18) have outstanding procoagulant effects, and the longer alkyl chain, the better procoagulant ability. However, N-alkylated chitosan cannot activate platelets, and its procoagulant mechanism remains to be further studied in future. Based on the above results, this paper makes a conjecture about the coagulation mechanism of N-alkylation chitosan.

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“…Aqueous acetic acid is generally used as a solvent for chitosan and on dissolution, amino groups of the chitosan become protonated making it positively charged [67]. It is biocompatible, biodegradable, mucoadhesive, easily available, nontoxic, inexpensive and organic solvents are not required for its solubilization [67]. The excellent muoadhesive properties of chitosan are due to the electrostatic interaction between positively charged chitosan and negatively charged mucosal surfaces [68].…”

Section: Chitosan and Its Derivativesmentioning

confidence: 99%

“…It is usually characterized by an average degree of deacetylation. Aqueous acetic acid is generally used as a solvent for chitosan and on dissolution, amino groups of the chitosan become protonated making it positively charged [67]. It is biocompatible, biodegradable, mucoadhesive, easily available, nontoxic, inexpensive and organic solvents are not required for its solubilization [67].…”

Section: Chitosan and Its Derivativesmentioning

confidence: 99%

Newer Trends in In Situ Gelling Systems for Controlled Ocular Drug Delivery

Jain¹,

Kumar²,

Singh³

et al. 2016

JAPLR

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In situ -gelling systems has the great potential in the wide areas of drug delivery. The application areas of such systems are broad and these systems can be formulated not only as gels but also as in situ gelling nanospheres, microspheres and liposomes. To overcome the drawbacks associated with conventional drug delivery systems and take advantages of both solutions and gels, such as accurate dosing, ease of administration of the former and longer precorneal residence of the latter, a newer concept of in situ gelling drug delivery systems was came in light for the first time in the early 1980s. In situ gelling systems are the polymeric solutions which can be administered in solution form and immediately undergoes sol-gel phase transition in to a viscoelastic gel upon exposure to physiological conditions (e.g. pH, temperature and ionic concentration) or application of external triggers. In situ gelling systems seems to have wide applications in ocular therapy. A lot of research is going on in this area proves the fact that in situ gelling systems can be advantageous in the ocular drug delivery. In the present review we will discuss recent researches involving application of in situ gelling polymers, their applications in the various domains of drug delivery and marketed formulations based on in situ gelling systems.

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Physicochemical properties and blood compatibility of acylated chitosan nanoparticles

Cited by 144 publications

References 26 publications

Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

Improving transfection efficiency of ultrapure oligochitosan/DNA polyplexes by medium acidification

Effect of Grafted Chain Length on Hemostatic Activity of N-alkylated Chitosan

Newer Trends in In Situ Gelling Systems for Controlled Ocular Drug Delivery

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