Physicochemical properties and immunological activities of polysaccharides from both crude and wine-processed Polygonatum sibiricum

Sun, Tingting; Zhang, Hong; Li, Ye; Liu, Yang; Dai, Wei; Fang, Jie; Cao, Chenchen; Die, Yun; Liu, Qian; Wang, Chunliu; Zhao, Lintao; Gong, Guiping; Wang, Zhongfu; Huang, Linjuan

doi:10.1016/j.ijbiomac.2019.11.166

Cited by 66 publications

(38 citation statements)

References 26 publications

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“…The constituents of P. sibiricum include polysaccharides, saponins, flavonoids, alkaloids, lignin, vitamins, and a variety of trace elements, of which polysaccharides are the major pharmacologically active ingredients [ 20 ]. In the last three years, Polygonatum sibiricum polysaccharides (PSP) are demonstrated to exhibit a wide range of pharmacological activity [ 21 ], such as osteogenic activity [ 22 ], anti-diabetes [ 23 ], immunological activity [ 24 ], and especially antioxidant activity, which makes them suitable for application in functional foods and therapeutic agents. PSP demonstrated strong antioxidant properties, which could attenuate D-gal-induced heart aging [ 25 ] and protect the mice livers against ethanol-induced oxidative damage via inhibiting oxidative stress [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Construction of Polygonatum sibiricum Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity

2022

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Although selenium nanoparticles (SeNPs) have attracted great attention due to their potential antioxidant activity and low toxicity, the application of SeNPs is still restricted by their poor stability. A combination of polysaccharides and SeNPs is an effective strategy to overcome the limitations. In this study, Polygonatum sibiricum polysaccharide (PSP) was used as a stabilizer to fabricate SeNPs under a simple redox system. Dynamic light scattering, transmission electron microscopy, energy dispersive X-ray, ultraviolet-visible spectroscopy, Fourier transform infrared, and X-ray photoelectron spectrometer were applied to characterize the synthesized PSP-SeNPs. The stability and the antioxidant activity of PSP-SeNPs were also investigated. The results revealed that the zero-valent and well-dispersed spherical PSP-SeNPs with an average size of 105 nm and a negative ζ-potential of −34.9 mV were successfully synthesized using 0.1 mg/mL PSP as a stabilizer. The prepared PSP-SeNPs were stable for 30 days at 4 °C. The decoration of the nanoparticle surface with PSP significantly improved the free radical scavenging ability of SeNPs. Compared to the H2O2-induced oxidative stress model group, the viability of PC-12 cells pretreated with 20 μg/mL PSP-SeNPs increased from 56% to 98%. Moreover, PSP-SeNPs exhibited a higher protective effect on the H2O2-induced oxidative damage on PC-12 cells and lower cytotoxicity than sodium selenite and SeNPs. In summary, these results suggest the great potential of PSP-SeNPs as a novel antioxidant agent in the food or nutraceuticals area.

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Section: Introductionmentioning

confidence: 99%

Construction of Polygonatum sibiricum Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity

2022

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“…PS processed by different methods have different biological functions, and a structural analysis of SPSP is vital to understand the structure-function relationship (34,35). Several previous studies showed that processed PS could enhance the effects on benefiting qi, nourishing blood as well as invigorating the spleen and kidney after processing (20,21). In this study, a BDS mice model established by hypodermic injection of APH and intraperitoneal injection of CTX was used to explore the blood tonification effects of SPSP.…”

Section: Discussionmentioning

confidence: 99%

“…In clinics, PS are mainly used as processed pieces. According to related reports, the type and content of polysaccharide in PS will change after processing ( 18 , 20 ).…”

Section: Discussionmentioning

confidence: 99%

“…For safe and effective applications, PS must be processed prior to clinical use, which usually processed by steam or rice wine to eliminate the side effects of numbing ( 19 ). A study had reported that the types and molar ratios of monosaccharides were different between wine-processed PS polysaccharides (WPSP) and crude PS polysaccharides (CPSP), and the immunological activity in vitro and vivo of WPSP was significantly better than that of CPSP ( 20 ). Clinical studies have demonstrated that steam-processed PS (SPS) could enhance the original function of “invigorating qi and blood, and tonifying the spleen, kidney and liver” ( 21 ).…”

Section: Introductionmentioning

confidence: 99%

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Blood-Enriching Effects and Immune-Regulation Mechanism of Steam-Processed Polygonatum Sibiricum Polysaccharide in Blood Deficiency Syndrome Mice

Wang

Yuan

et al. 2022

Front. Immunol.

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Polygonatum sibiricum Red. has been used as a medicinal herb and nutritional food in traditional Chinese medicine for a long time. It must be processed prior to clinical use for safe and effective applications. However, the present studies mainly focused on crude Polygonatum sibiricum (PS). This study aimed to investigate the chemical properties, blood-enriching effects and mechanism of polysaccharide from the steam-processed Polygonatum sibiricum (SPS), which is a common form of PS in clinical applications. Instrumentation analyses and chemistry analyses revealed the structure of SPS polysaccharide (SPSP). A mice model of blood deficiency syndrome (BDS) was induced by acetylphenylhydrazine (APH) and cyclophosphamide (CTX). Blood routine test, spleen histopathological changes, serum cytokines, etc. were measured. The spleen transcriptome changes of BDS mice were detected by RNA sequencing (RNA-seq). The results showed that SPSP consists predominantly of Gal and GalA together with fewer amounts of Man, Glc, Ara, Rha and GlcN. It could significantly increase peripheral blood cells, restore the splenic trabecular structure, and reverse hematopoietic cytokines to normal levels. RNA-seq analysis showed that 122 differentially expressed genes (DEGs) were obtained after SPSP treatment. GO and KEGG analysis revealed that SPSP-regulated DEGs were mainly involved in hematopoiesis, immune regulation signaling pathways. The reliability of transcriptome profiling was validated by quantitative real-time PCR and Western blot, and the results indicated that the potential molecular mechanisms of the blood-enriching effects of SPSP might be associated with the regulating of JAK1-STAT1 pathway, and elevated the hematopoietic cytokines (EPO, G-CSF, TNF-α and IL-6). This work provides important information on the potential mechanisms of SPSP against BDS.

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“…Among them, polysaccharides and phytosterols are the most abundant medicinal components. P. sibiricum polysaccharide (PSP) is mainly composed of galactose, mannose, glucose and galacturonic acid, in molar ratios of 29.63:36.10:15.09:10.20 ( 22 ). PSP has been verified to exert a variety of medicinal functions, including anti-inflammatory ( 23 ) and anti-tumor effects ( 24 ), lowering blood glucose ( 25 ), enhancing immunity and promoting the differentiation of bone cells ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD‑induced mouse obesity via regulation of lipid metabolism and inflammatory response

et al. 2021

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The present study sought to elucidate the role of Polygonatum sibiricum F. Delaroche polysaccharide (PSP) in high-fat diet (HFD)-induced mouse obesity and investigated the primary molecular mechanism underlaying these effects. An obese mouse model was established by feeding HFD and three doses of PSP were administered intragastrically. Changes in body weight, serum lipids and parameters were recorded and the mechanism was explored by reverse transcription-quantitative PCR and western blotting. Body weight, blood lipids, blood glucose, insulin, resistin, adiponectin, liver weight and abdominal fat pads weight were reduced by PSP and abnormal expression levels of inflammatory factors such as TNF-α, IL-6, IL-1β and iNOS and lipid metabolism genes such as FAS, SREBP-1, PPARα and CPT-1were also reversed by PSP. The 5′ adenosine monophosphate-activated protein kinase (AMPK) signaling pathway was activated in PSP mouse liver, leading to lipid-lowering and anti-inflammatory effects. The results therefore suggested that PSP exhibited lipid-lowering and anti-inflammatory effects by activating the AMPK signaling pathway.

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Physicochemical properties and immunological activities of polysaccharides from both crude and wine-processed Polygonatum sibiricum

Cited by 66 publications

References 26 publications

Construction of Polygonatum sibiricum Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity

Construction of Polygonatum sibiricum Polysaccharide Functionalized Selenium Nanoparticles for the Enhancement of Stability and Antioxidant Activity

Blood-Enriching Effects and Immune-Regulation Mechanism of Steam-Processed Polygonatum Sibiricum Polysaccharide in Blood Deficiency Syndrome Mice

Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD‑induced mouse obesity via regulation of lipid metabolism and inflammatory response

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