“…The yield of polysaccharides no longer increased with time, possibly because the enzyme reacted entirely with a certain amount of substrate at the early stage of the reaction. The increase in reaction time at the later stage could not continue to improve the yield [21] . Fig.…”
“…The yield of polysaccharides no longer increased with time, possibly because the enzyme reacted entirely with a certain amount of substrate at the early stage of the reaction. The increase in reaction time at the later stage could not continue to improve the yield [21] . Fig.…”
“…The HB seeds were first pearled at a 20% pearling rate and then milled in a cyclone grinder to obtain highland barley flour (HBF), which was finally sieved through 100-mesh. The HBS was extracted from the abovementioned HBF by a double-enzyme method in the laboratory; the detailed method was referred to the scheme of Nie et al [ 46 ]. The HBF (100 g) was combined with 600 mL of distilled water.…”
Section: Methodsmentioning
confidence: 99%
“…The HBS and HBS/HBPs paste made by RVA were transferred to some 10 mL beakers and held at 4 °C for 6 and 12 h after cooling to room temperature. The experimental parameters were 5 g trigger force, 50% strain and the speed was 1.0 mm/s before, during and after the test [ 46 ].…”
This study evaluated the effects of four highland barley proteins (HBPs), namely, albumin, globulin, gliadin and glutenin, on the short-term retrogradation of highland barley starch (HBS). The findings reveal that HBPs could reduce the viscosity, storage modulus and hardness of HBS, with albumin and globulin showing more prominent effects. Furthermore, with the addition of HBPs, the loss tangent (tan δ) of HBS loss increased from 0.07 to 0.10, and the enthalpy of gelatinization decreased from 8.33 to 7.23. The degree of retrogradation (DR%) of HBS was 5.57%, and the DR% decreased by 26.65%, 38.78%, 11.67% and 20.29% with the addition of albumin, globulin, gliadin and glutenin, respectively. Moreover, the relative crystallinity (RC) and the double helix structures were inhibited with the HBPs’ incorporation. Meanwhile, the HBPs also could inhibit water migration and improve the structure of HBS gels. In summary, HBPs could inhibit the retrogradation behavior of HBS, which provides new theoretical insights for the production studies of highland barley foods.
“…At 150 minutes, the polysaccharide yield was the highest, reaching 8.295%. Due to the mechanical damage caused by ultrasound to large molecules, the molecular structures of polysaccharides will be destroyed with the extension of ultrasound time, resulting in a decrease in polysaccharide yield with the extension of time [41]. Therefore, the optimal process for ultrasound-assisted extraction of Tenebrio molitor polysaccharides was determined to be 150 minutes.…”
Section: Effect Of Time On the Yield Of Tenebrio Molitor Polysaccharidementioning
[Objective]The study aimed to extract polysaccharides from Tenebrio molitor using ultrasound-assisted extraction and assess their structural characteristics and antioxidant activity.
[Methods]Defatted Tenebrio molitor was utilized, with extraction temperature, time, ultrasonic power, and liquid-to-material ratio varied. Response surface experiments determined optimal extraction parameters, yielding 9.513% polysaccharides.
[Result] Infrared spectroscopy revealed pyranose structure with predominant C-O, C=O, and O-H functional groups. Antioxidant activity was evaluated by scavenging DPPH·, OH·, and ABTS+· radicals and Fe3+ reduction. Polysaccharide concentration positively correlated with radical scavenging abilities. Compared to Vitamin C, polysaccharides exhibited stronger scavenging of DPPH· and OH·, moderate scavenging of ABTS+·, and Fe3+ reduction, with IC50 values of 0.9625, 9.1909, and 235.69 mg/mL respectively. Notably, Fe3+ reduction peaked at 1.6 mg/mL with an absorbance of 0.38899.
[Conclusion] Tenebrio molitor polysaccharides demonstrated substantial antioxidant activity, suggesting their potential in various applications. This research provides valuable insights for leveraging Tenebrio molitor as a functional ingredient, contributing to its development and utilization.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
