Physicochemical properties of chitosan-poly(ethylene oxide) hydrogel modified through linoleic acid

Abstract

A new chitosan-poly(ethylene oxide) (PEO) hydrogel was fabricated and its physicochemical properties were evaluated for its potential applications in medical devices. Chitosan-linolate was synthesized by grafting linoleic acid via N-(3-diethylpropyl)-N'-ethylcarbodiimide chemistry to the amine groups in chitosan. Spontaneous formation of the chitosan-PEO gel was achieved by mixing the chitosan-linolate solution with a PEO-thiol solution using a Michael type addition reaction. The chemical properties of both chitosan-linolate polymer and chitosan-PEO hydrogel were analyzed by FTIR, NMR, and XPS. The physical properties of the hydrogel were evaluated by testing the rheological properties of the precursor solutions with a rheometer by measuring the degree of swelling and degradation of the chitosan-PEO hydrogel and by observing the dehydrated morphology by SEM. Although the rheological tests demonstrated a process of spontaneous formation of the chitosan-PEO hydrogel, measurements of gel swelling in either acidic or basic media and an observation of its morphology by SEM demonstrated its physical and morphological stabilization in water for more than 3 months. On the other hand, the chitosan-PEO hydrogel degraded quicker in a basic phosphate buffer medium than in either acid or neutral media.

Effects of maintaining temperature and time on properties and structures of poly(dimethyldiallylammonium chloride)

“…Synthesis of chitosan–PEO hydrogel was previously described in detail in our research group 12, 13, 22. In brief, after grafting chitosan with CEA through EDC chemistry (chitosan‐acrylate), chitosan–PEO hydrogel was obtained via Michael type addition reaction by mixing separate 10% precursor solutions of chitosan‐acrylate and PEO‐thiols.…”

“…Among the carrier polymers, chitosan, a linear polysaccharide composed of randomly distributed β‐(1–4)‐linked D ‐glucosamine and N‐acetyl‐ D ‐glucosamine, has been attractive to these ends for its combined properties of biocompatibility, biodegradability, minimal toxicity, and promotion of tissue regeneration 9–14. It has been used in various forms such as carriers for cell delivery and therapeutics, as well as polymeric scaffolds in either porous or films for its applications in bone tissue engineering because of its promotion of growth and mineral rich matrix deposition by osteoblasts in culture 15–17.…”

Bioactive-Loaded Hydrogels Based on Bacterial Nanocellulose, Chitosan, and Poloxamer for Rebalancing Vaginal Microbiota

“…Besides poloxamer and cellulose derivates, chitosan (CS), which is a linear polysaccharide, is also frequently used in various formulations for temperature-and pH-sensitive hydrogels with biomedical applications [17,[35][36][37][38][39][40]. Several studies showed the potential of chitosan as a protective agent against pathogenic bacteria, but it also has anti-inflammatory properties, stimulating the wound-healing process [41][42][43][44][45].…”

Effects of recombinant human bone morphogenic protein‐2 and human bone marrow‐derived stromal cells on in vivo bone regeneration of chitosan–poly(ethylene oxide) hydrogel

“…Among the carrier polymers, chitosan, a linear polysaccharide composed of randomly distributed b-(1-4)linked D-glucosamine and N-acetyl-D-glucosamine, has been attractive to these ends for its combined properties of biocompatibility, biodegradability, minimal toxicity, and promotion of tissue regeneration. [9][10][11][12][13][14] It has been used in various forms such as carriers for cell delivery and therapeutics, as well as polymeric scaffolds in either porous or films for its applications in bone tissue engineering because of its promotion of growth and mineral rich matrix deposition by osteoblasts in culture. [15][16][17] Chitosan demonstrated its biodegradation and biocompatibility with minimal local inflammation and osteoconduction through porous structures.…”