Physicochemical Properties of Complexes between Deoxyribonucleic Acid and Antibiotics Which Affect Ribonucleic Acid Synthesis (Actinomycin, Daunomycin, Cinerubin, Nogalamycin, Chromomycin, Mithramycin, and Olivomycin)<sup>*</sup>

Kersten, W.; Kersten, H.; Szybalski, Waclaw; Fiandt, M.

doi:10.1021/bi00865a031

Cited by 303 publications

(81 citation statements)

References 25 publications

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“…The effects on viscosity shown are qualitatively consistent with previous reports 15, 7,151. The intrinsic viscasities of the complexes increase with '7' up to "r" values (about 0.16-0.20) corresponding closely both to the apparent number of binding sites per nucleotide (r = 0.12), obtained by extrapolation of the curve for small values of "r", in the spectrophotometric titration of daunomycin with DNA 1141 and to the molar ratio drug/nucleotides (about 0.12-0.16) recovered by ureagradient, in DNA cellulose column (table 1).…”

Section: Resultssupporting

confidence: 92%

“…Daunomycin-induced increase in viscosity and decrease in sedimentation of native DNA are consistent with the intercalation hypothesis. Other authors reported an increase in the relative viscosity [S, 151 and reduced specific viscosity [7] high molecular weight. Although a provisional interpretation of this increase was attempted, it could not be securely based, because the points on the plot of the relative (or reduced} viscosity as a function of drug concentration correspond to DNA attached to different amounts (r) of antibiotic.…”

Section: Resultsmentioning

confidence: 96%

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Studies on the mode of interaction of daunomycin with DNA

Zunino¹

1971

FEBS Letters

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 96%

Studies on the mode of interaction of daunomycin with DNA

Zunino¹

1971

FEBS Letters

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“…Similar purification techniques work with various satellites of eukaryotic DNA (3). The efficacy of the actinomycin purification can be explained by the low affinity of the drug for high-AT content DNA (10). The 5S DNA behaves as a homogeneous DNA component when bulk X. laevis DNA is complexed with silver or mercury and banded in Cs2SO4, or when it is banded in neutral or alkaline CsCl, or when it is complexed with actinomycin D in neutral CsCl.…”

Section: Discussionmentioning

confidence: 99%

Purification and Some Characteristics of 5S DNA from Xenopus laevis

Brown

Wensink

Jordan

1971

Proc. Natl. Acad. Sci. U.S.A.

150

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DNA containing the multiple genes for 5S RNA The small RNA component of ribosomes, termed 5S RNA, comprises about 1-2% of total cellular RNA in prokaryotes and eukaryotes. In the South African toad, Xenopus laevis, 5S RNA genes are known to be in greater abundance than the genes for 18S and 28S ribosomal RNA and are not intermingled with them (1). This report documents the purification of the DNA containing the genes for 5S RNA from the genome of X. laevis and describes some characteristics of 5S DNA. MATERIALS AND METHODSCrude Cs2SO4 was purchased from Gallard Schlessinger (more than 99% pure, catalogue no. 27573) and was found to be as good for the experiments described here as the much more expensive optical-grade product. CsCl was purchased from Harshaw, and actinomycin D was a gift of Merck. The antibiotic was dissolved in a stock aqueous solution (1 mg/ml) and stored at 4°C in the dark.Purification of DNA. DNA was purified from isolated nuclei of X. laevis erythrocytes (1). To maintain the DNA at the highest possible molecular weight, it was never precipitated during purification. The purified nuclei, suspended in SSC (0.15 M NaCl-0.015 M sodium citrate), were lysed with 0.5% sodium dodecyl sulfate and digested with 1 mg/ml of Pronase overnight at 370C. The DNA was extracted gently with phenol three or four times. Fig. la).For saturation-hybridization experiments, about 1.5 mg of 5S RNA isolated from ovaries of X. laevis was methylated in vitro with 5 mCi of [3H]dimethyl sulfate (6). The 5S [3H]RNA was purified by four ethanol precipitations that removed all acid-soluble radioactivity; the specific activity of the RNA was 10,000 cpm/ug.Radioactive RNA was hybridized with DNA immobilized on Millipore HA filters. Aliquots of DNA complexed with silver were mixed with 10 volumes of 20 X SSC to complex the silver before the DNA was denatured and trapped on filters.The hydribization medium contained 50% formamide, 4 X SSC, and 0.05 M Tris (pH 7.8); incubation took place at 40'C overnight (7). The filters were washed, treated with RNase, and counted in a scintillation counter.Denaturation Map of 5S DNA. The 5S DNA was partially denatured with alkali for electron microscopy (8). The exact procedure that we used for 5S DNA has been described in detail for ribosomal DNA (9). RESULTSPurification of 5S DNA. Two characteristics of 5S DNA from X. laevis made its isolation possible. First, hybridization experiments have shown that thousands of genes for 5S RNA are present in a haploid complement of X. laevis DNA, so that 5S DNA is abundant in the genome (ref. 1, and see Table 2). Second, 5S DNA has a lower buoyant density in CsCl than does the bulk of X. laevis DNA (1) [however, this difference (about 7 mg/cm8) is not enough to allow its separa-

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“…This inhibition arises from an intercalative binding mode (5) and it is known that daunomycin and adriamycin intercalate into double-stranded DNA, although these drugs do not show any requirement for a particular base at the intercalation site (1)(2)(3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Antitumor activity at the molecular level: binding modes in anthracycline–dinucleotide complexes as determined by optical titration and magnetic circular dichroism

Bell¹,

Buchanan²,

Hollebone³

et al. 1982

Can. J. Chem.

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. Can. J. Chem. 60,291 (1982). The first application of magnetic circular dichroism (rncd) spectroscopy to drug-dinucleotide binding is reported. The mcd spectra have been recorded for complexes of the intercalating anthracyclines carrninomycin, marcellomycin, and aclacinomycin with the dinucleotide CpG. Analysis indicates that drugs having OH groups peri to each anthraquinone carbonyl (i.e. carminomycin and marcellomycin) bind with only one drug molecule in the central hydrophilic region of the nucleotide. By contrast, aclacinomycin, in which one of the anthraquinone carbonyls lacks a peri-OH, is found to cooperatively bind, and has two drug molecules present in the dinucleotide cavity. The cooperativity of binding has been elucidated via an optical titration procedure. Chem. 60, 291 (1982). On rapporte la premiere application de la spectroscopie du dichroisme circulaire magnetique (dcm) a la liaison medicarnent-dinucleotide. On a enregistre les spectres de dcm des complexes formes par les intercalants anthracycline, carminomycine, marcellomycine et aclacinomycine, avec le dinucleotide CpG. Les analyses revelent que les medicaments qui ont des groupements OH enpPri par rapport ?+ chaque carbonyle de l'anthraquinone (comme la carminomycine et la marcellomycine) sont lies a une seule molecule de medicament dans la region hydrophile centrale du nucleotide. On trouve au contraire que I'aclacinomycine, dans laquelle il manque un groupement OH peri pour I'un des carbonyles de l'anthraquinone, est liee cooperativement et que deux moltcules de medicament sont presentes dans la cavite du dinucleotide. On a identifie la cooperativite du lien par I'intermedaire d'une methode de titration optique.[Traduit par ie journal]

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Physicochemical Properties of Complexes between Deoxyribonucleic Acid and Antibiotics Which Affect Ribonucleic Acid Synthesis (Actinomycin, Daunomycin, Cinerubin, Nogalamycin, Chromomycin, Mithramycin, and Olivomycin)^*

Cited by 303 publications

References 25 publications

Studies on the mode of interaction of daunomycin with DNA

Studies on the mode of interaction of daunomycin with DNA

Purification and Some Characteristics of 5S DNA from Xenopus laevis

Antitumor activity at the molecular level: binding modes in anthracycline–dinucleotide complexes as determined by optical titration and magnetic circular dichroism

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Physicochemical Properties of Complexes between Deoxyribonucleic Acid and Antibiotics Which Affect Ribonucleic Acid Synthesis (Actinomycin, Daunomycin, Cinerubin, Nogalamycin, Chromomycin, Mithramycin, and Olivomycin)*

Cited by 303 publications

References 25 publications

Studies on the mode of interaction of daunomycin with DNA

Studies on the mode of interaction of daunomycin with DNA

Purification and Some Characteristics of 5S DNA from Xenopus laevis

Antitumor activity at the molecular level: binding modes in anthracycline–dinucleotide complexes as determined by optical titration and magnetic circular dichroism

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Product

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Physicochemical Properties of Complexes between Deoxyribonucleic Acid and Antibiotics Which Affect Ribonucleic Acid Synthesis (Actinomycin, Daunomycin, Cinerubin, Nogalamycin, Chromomycin, Mithramycin, and Olivomycin)^*