Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids

Abstract

The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M. A completely randomized design and the least significant difference test were used in the experimental design, and all measurements were performed in triplicate. The highest yield (29.17%) was obtained from pretreatment with 0.9 M HCl. The gel strength did not differ significantly (p>0.05) according to acid type (280.26-259.62 g Bloom), and the highest viscosity was obtained from the 0.6 M citric acid pretreatment. All the gelatins contained α- and β-chain components and several degraded peptides (24-66 kDa). The color and Fourier-transform infrared spectrum of the gelatin extracted using 0.9 M HCl were similar to those of commercial bovine skin gelatin. In general, the physicochemical properties of the gelatin complied with the industry standard set by the Gelatin Manufacturers Institute of America, revealing that buffalo hide could serve as a potential alternative source of gelatin.

Physical and Chemical Characteristic of Halal Gelatin Extracted from Buffalo Hide with Addition of Pineapple Rind at Different Ratio

“…The similar phenomenon also reported by Mulyani et al (2017) that the yield percentage of gelatin did not significantly increased when buffalo hide was soaked in a different acid (hydrochloric citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M, respectively). The yield percentage ranged from 6.30-29.27%.…”

“…On the other hand, gelatin from bone and hide of cattle contain primarily calcium salts of those acids which are used in the neutralization after liming. The ash content of gelatin in this research was lower than those from Mulyani et al (2017). The ash content of gelatin from buffalo hide was soaked in a different acid (hydrochloric, citric, and acetic acids at concentrations of 0.9, 0.5, and 1.5 M) with values of 0.56%, 0.62% and 2.67%, respectively.…”

“…The moisture of gelatin in this research met the moisture standard in GMIA, ranged from 8-13%. The moisture of gelatin in this research was differ than those from Mulyani et al (2017). The moisture of gelatin from buffalo hide was soaked in a different acid (hydrochloric, citric, and acetic acids at concentrations of 0.9, 0.5, and 1.5 M) with values of 7.08%, 4.41% and 11.09%, respectively.…”

“…According to Remawati (2016), the gelatin derived bovine hide that extracted by acetic acid 0.2 M had value of viscosity 30 cP, yield was 6.29 %, pH value was 5.6, water content 63.51 %, and ash content was 0.3%. Mulyani et al, (2017) had studied on gelatin producing from buffalo hide by using different acid solution (hydrochloric acid, citric acid and acetic acid) at various concentrations. The produced yield percentages were 6.22%, 6.52% and 6.79%, respectively.…”

Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates

“…e obtained gelatin contained protein (96.58%), lipid (1.27%), and ash (1.90%). e gelatin yield reached 52.97%, which is much higher than the reported extraction rates of many other animal gelatins [28][29][30]. e reason might be that yak skin contained more proteins and less lipids since yaks live in high altitudes with extremely cold climates [31].…”