1975
DOI: 10.1083/jcb.67.2.378
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Physicochemical properties of kinetoplast DNA from Crithidia acanthocephali. Crithidia luciliae, and Trypanosoma lewisi.

Abstract: The protozoa Crithidia and Trypanosoma contain within a mitochondrion a mass of DNA known as kinetoplast DNA (kDNA) which consists mainly of an association of thousands of small circular molecules of similar size held together by topological interlocking. Using kDNA from Crithidia acanthocephali, Crithidia luciliae, and Trypanosoma lewisi, physicochemical studies have been carried out with intact associations and with fractions of covalently closed single circular molecules, and of open single circular and uni… Show more

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Cited by 38 publications
(27 citation statements)
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“…Ovaries from approximately 300 flies of a single species were thawed and lysed by addition of sodium dodecyl sulfate to 2%, and the lysate was subjected to preparative CsCI equilibrium density gradient centrifugation (10). The buoyant densities of individual fractions of the resuiting gradient were determined from their refractive indices, and the configurations of the DNA molecules each fraction contained were determined by electron microscopy.…”
Section: Methodsmentioning
confidence: 99%
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“…Ovaries from approximately 300 flies of a single species were thawed and lysed by addition of sodium dodecyl sulfate to 2%, and the lysate was subjected to preparative CsCI equilibrium density gradient centrifugation (10). The buoyant densities of individual fractions of the resuiting gradient were determined from their refractive indices, and the configurations of the DNA molecules each fraction contained were determined by electron microscopy.…”
Section: Methodsmentioning
confidence: 99%
“…DNA samples comprising 50-80% open circular molecules (each containing at least one phosphodiester bond break) used for thermal denaturation studies were obtained as the upper fluorescent band in a CsCI/ethidium bromide equilibrium density gradient (10,11) of pooled circular-molecule-containing fractions obtained by CsCl centrifugation of ovary lysates. Wolstenholme et al (12).…”
Section: Methodsmentioning
confidence: 99%
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“…High molecular weight DNA was extracted from whole cells by the same procedures as used to prepare D. melanogaster DNA. Nuclear DNA was then purified from these preparations of total cellular DNA (nuclear and kinetoplast DNA) by repeated sedimentation through a 20% sucrose solution as described previously (6).…”
Section: Methodsmentioning
confidence: 99%