Physics at SATURNE

Arvieux, J.

doi:10.1007/978-3-642-75467-8_19

“…During an immune response the complement classical pathway is activated, and the nascent C3b reactive acyl group locally contracts a covalent bond with an antigen or an antigen-antibody complex [ 101. Previous studies used chemically linked complexes [18] to study the role of antigen-C3b complexes in a specific immune response. The covalent C3b-IgG complexes we used illustrate the natural antigen-C3b complexes better.…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, C3b is endowed with non-covalent binding capacities towards complement receptors on antigen-presenting cells (APC) [16, 17l.This double reactivity of C3b is in favor of a pivotal role in the specific immune response [l]. Previous experiments using chemical cross-linking between C3b and antigen demonstrated an enhancement of antigen presentation by antigen-bound C3b [18].…”

Section: Introductionmentioning

confidence: 99%

Covalent binding of C3b to monoclonal antibodies selectively up‐regulates heavy chain epitope recognition by T cells

Santoro

¹

,

Drouet

²

,

Reboul

³

et al. 1994

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Protein C3 of the complement system is known for its role in the nonspecific immune response. Covalent binding of C3b to antigen upon complement activation also plays a significant role in specific T cell immune response. C3b-antigen complexes can bind to complement receptors on the antigen-presenting cell, and the C3b antigen link (most often an ester link) remains fairly stable inside the cells. In this study, IgG1,kappa and IgG2a,kappa murine monoclonal antibodies (mAb) were used as antigens; covalent complexes between mAb and C3b were produced and purified in vitro from purified proteins; human B cell lines and T cell clones were raised from tumor patients who received mAb injections for cancer therapy or diagnosis. Recognition of epitopes of these mAb by T cell clones when the mAb were processed alone or bound to C3b was compared. IgG or IgG-C3b complexes presented by B cell lines were able to stimulate proliferation of kappa light chain-specific T cell clones at similar concentrations. In contrast, IgG-C3b complex recognition by heavy chain-specific T cell clones required 100-fold less IgG-C3b than uncomplexed IgG. As C3b was shown to be covalently bound only to the IgG heavy chains in the complexes, C3b chaperoning is restricted to only the IgG heavy chain and selectively influences intracellular steps of IgG heavy chain processing. This differential modulation of C3b suggests an early dissociation of IgG heavy and light chains in antigen-presenting cells.

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“…The DISTO collaboration has constructed a largeacceptance magnetic spectrometer to provide broad kinematic coverage of multi-particle final states involving charged particles produced in pp scattering. Examples include the measurement of polarization observables in reactions such as pp → pK + Y , with Y representing a hyperon or a hyperon resonance (Λ, Σ 0 or Y * ), and the study of vector meson (φ, ω and ρ) production in pp interactions [1][2][3][4][5][6]. These measurements are now in progress at Laboratoire Nationale Saturne (LNS) in Saclay.…”

Section: Introductionmentioning

confidence: 99%

The DISTO data acquisition system at SATURNE

Balestra

¹

,

Bedfer

²

,

Bertini³

et al. 1998

IEEE Trans. Nucl. Sci.

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The DISTO collaboration has built a large-acceptance magnetic spectrometer designed to provide broad kinematic coverage of multi-particle final states produced in pp scattering. The spectrometer has been installed in the polarized proton beam of the Saturne accelerator in Saclay to study polarization observables in the pp → pK + Y (Y = Λ, Σ 0 or Y * ) reaction and vector meson production (φ, ω and ρ) in pp collisions. The data acquisition system is based on a VME 68030 CPU running the OS/9 operating system, housed in a single VME crate together with the CAMAC interface, the triple port ECL memories, and four RISC R3000 CPU. The digitization of signals from the detectors is made by PCOS III and FERA front-end electronics. Data of several events belonging to a single Saturne extraction are stored in VME triple-port ECL memories using a hardwired fast sequencer. The buffer, optionally filtered by the RISC R3000 CPU, is recorded on a DLT cassette by DAQ CPU using the on-board SCSI interface during the acceleration cycle. Two UNIX workstations are connected to the VME CPUs through a fast parallel bus and the Local Area Network. They analyze a subset of events for on-line monitoring. The data acquisition system is able to read and record 3500 ev/burst in the present configuration with a dead time of 15%.

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“…The DISTO collaboration has constructed a largeacceptance magnetic spectrometer to provide broad kinematic coverage of multi-particle final states involving charged particles produced in pp scattering. Examples include the measurement of polarization observables in reactions such as pp → pK + Y , with Y representing a hyperon or a hyperon resonance (Λ, Σ 0 or Y * ), and the study of vector meson (φ, ω and ρ) production in pp interactions [1][2][3][4][5][6]. These measurements are now in progress at Laboratoire Nationale Saturne (LNS) in Saclay.…”

Section: Introductionmentioning

confidence: 99%

The DISTO first level trigger at SATURNE

Balestra

¹

,

Bedfer²,

Bertini

³

et al. 1998

IEEE Trans. Nucl. Sci.

2

0

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The DISTO collaboration has built a large-acceptance magnetic spectrometer designed to provide broad kinematic coverage of multi-particle final states produced in pp scattering. The spectrometer has been installed in the polarized proton beam of the Saturne accelerator in Saclay to study polarization observables in the pp → pK + Y (Y = Λ, Σ 0 or Y * ) reaction and vector meson production (φ, ω and ρ) in pp collisions. The common signature of such events is the multiplicity of four charged particles in the final state. A flexible 1st level trigger which uses topological information from fast detectors (scintillating fibers and hodoscope) has been built. It is completely software programmable through a menu-driven user interface and allows switching between production and monitor triggers on successive beam spills.

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Physics at SATURNE

Cited by 3 publications

References 25 publications

Covalent binding of C3b to monoclonal antibodies selectively up‐regulates heavy chain epitope recognition by T cells

Covalent binding of C3b to monoclonal antibodies selectively up‐regulates heavy chain epitope recognition by T cells

The DISTO data acquisition system at SATURNE

The DISTO first level trigger at SATURNE

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