Physics of bioprinting

Shafiee, Ashkan; Ghadiri, Elham; Ramesh, Haripriya; Kengla, Carlos; Kassis, Jareer; Calvert, Paul; Williams, David; Khademhosseini, Ali; Narayan, Roger J.; Forgács, Gabor; Atala, Anthony

doi:10.1063/1.5087206

Cited by 41 publications

(24 citation statements)

References 107 publications

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“…For more than 15 years, various printing methods for the transfer of biomaterials have been studied[ 56 , 59 ]. Regarding laser printing methods, they have the best resolution as well as provide cell survival during the transfer at the level of 95% or more[ 10 , 56 ].…”

Section: Decision Tree For Controlled Hydrogel Transfermentioning

confidence: 99%

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Yusupov¹,

Churbanov²,

Churbanova³

et al. 2024

IJB

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Laser-induced forward transfer is a versatile, non-contact, and nozzle-free printing technique which has demonstrated high potential for different printing applications with high resolution. In this article, three most widely used hydrogels in bioprinting (2% hyaluronic acid sodium salt, 1% methylcellulose, and 1% sodium alginate) were used to study laser printing processes. For this purpose, the authors applied a laser system based on a pulsed infrared laser (1064 nm wavelength, 8 ns pulse duration, 1 – 5 J/cm2 laser fluence, and 30 μm laser spot size). A high-speed shooting showed that the increase in fluence caused a sequential change in the transfer regimes: No transfer regime, optimal jetting regime with a single droplet transfer, high speed regime, turbulent regime, and plume regime. It was demonstrated that in the optimal jetting regime, which led to printing with single droplets, the size and volume of droplets transferred to the acceptor slide increased almost linearly with the increase of laser fluence. It was also shown that the maintenance of a stable temperature (±2°C) allowed for neglecting the temperature-induced viscosity change of hydrogels. It was determined that under room conditions (20°C, humidity 50%), the hydrogel layer, due to drying processes, decreased with a speed of about 8 μm/min, which could lead to a temporal variation of the transfer process parameters. The authors developed a practical algorithm that allowed quick configuration of the laser printing process on an applied experimental setup. The configuration is provided by the change of the easily tunable parameters: Laser pulse energy, laser spot size, the distance between the donor ribbon and acceptor plate, as well as the thickness of the hydrogel layer on the donor ribbon slide.

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Section: Decision Tree For Controlled Hydrogel Transfermentioning

confidence: 99%

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Yusupov¹,

Churbanov²,

Churbanova³

et al. 2024

IJB

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“…Additive manufacturing, specifically printing technologies, have been employed in many different applications ranging from electronic device fabrication [ 17 , 18 ], including transistors [ 19 ], light emitting diodes [ 20 ], and solar cells [ 21 ] to biofabrication [ 22 , 23 , 24 , 25 ], tissue engineering [ 26 , 27 , 28 ], and optimizing the surgical planning [ 29 ]. For example, in drug delivery systems, inkjet printing can provide a platform with which to engineer the surface properties of the host material in order to obtain zero-order release kinetics [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Inkjet Printing of Synthesized Melanin Nanoparticles as a Biocompatible Matrix for Pharmacologic Agents

et al. 2020

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Melanin is a natural biopigment that is produced by melanocytes and can be found in most living organisms. The unique physical and chemical properties of melanin render it potentially useful for numerous applications, particularly those in which a biocompatible functional material is required. Herein, we introduce one important technology in which melanin can be utilized: a drug delivery system in terms of a biocompatible matrix. However, extracting melanin from different biological sources is costly and time-consuming and introduces variabilities in terms of chemical structure, properties, and functions. Hence, a functionally reproducible system is hard to achieve using biologically extracted melanin. Here we report the synthesis of melanin nanoparticles of controlled uniform sizes and chemical characteristics. The optical, chemical, and structural characteristics of synthesized nanoparticles were characterized by optical confocal photoluminescence (PL) imaging, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Zeta potentiometry. The melanin nanoparticles have 100 nm size and a narrow size distribution. The advantage of a nanoparticle structure is its enhanced surface-to-volume ratio compared to bulk pigments, which is important for applications in which controlling the microscopic surface area is essential. Using the inkjet printing technique, we developed melanin thin films with minimum ink waste and loaded them with methylene blue (our representative drug) to test the drug-loading ability of the melanin nanoparticles. Inkjet printing allowed us to create smooth uniform films with precise deposition and minimum ink-waste. The spectroscopic analysis confirmed the attachment of the “drug” onto the melanin nanoparticles as a matrix. Hence, our data identify melanin as a material system to integrate into drug release applications.

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“…The absorption spectra were obtained by sweeping wavelengths from 900 to 250 nm. The respective wavelengths in the cross point between the onset of the spectra and the baseline were used to calculate the E g using Equation (1). It is worth noting that an insulator that is colored can cause a false positive reading as a semiconductor.…”

Section: Resultsmentioning

confidence: 99%

“…Tissue engineering uses physical and biological sciences, as well as biomedical technology to enhance human life by providing human tissues and eventually organs [1,2]. Advancements in tissue engineering, based on its underlying science [3] and technology [4], have opened new avenues for clinical applications [5].…”

Section: Introductionmentioning

confidence: 99%

Energy Band Gap Investigation of Biomaterials: A Comprehensive Material Approach for Biocompatibility of Medical Electronic Devices

et al. 2020

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Over the past ten years, tissue engineering has witnessed significant technological and scientific advancements. Progress in both stem cell science and additive manufacturing have established new horizons in research and are poised to bring improvements in healthcare closer to reality. However, more sophisticated indications such as the scale-up fabrication of biological structures (e.g., human tissues and organs) still require standardization. To that end, biocompatible electronics may be helpful in the biofabrication process. Here, we report the results of our systematic exploration to seek biocompatible/degradable functional electronic materials that could be used for electronic device fabrications. We investigated the electronic properties of various biomaterials in terms of energy diagrams, and the energy band gaps of such materials were obtained using optical absorption spectroscopy. The main component of an electronic device is manufactured with semiconductor materials (i.e., Eg between 1 to 2.5 eV). Most biomaterials showed an optical absorption edge greater than 2.5 eV. For example, fibrinogen, glycerol, and gelatin showed values of 3.54, 3.02, and 3.0 eV, respectively. Meanwhile, a few materials used in the tissue engineering field were found to be semiconductors, such as the phenol red in cell culture media (1.96 eV energy band gap). The data from this research may be used to fabricate biocompatible/degradable electronic devices for medical applications.

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Physics of bioprinting

Cited by 41 publications

References 107 publications

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Inkjet Printing of Synthesized Melanin Nanoparticles as a Biocompatible Matrix for Pharmacologic Agents

Energy Band Gap Investigation of Biomaterials: A Comprehensive Material Approach for Biocompatibility of Medical Electronic Devices

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